Biochemical significance of the non-common amino acid sequences specifying the common functions of enzymes, polysaccharide lyases

指定酶、多糖裂解酶的常见功能的非常见氨基酸序列的生化意义

基本信息

  • 批准号:
    14360052
  • 负责人:
  • 金额:
    $ 8.51万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2004
  • 项目状态:
    已结题

项目摘要

Polysaccharide lyase is an enzyme catalyzing the depolymerization of polysaccharides such as alginate, xanthan, gellan, and hyarunonate, all of which are produced by bacteria, and gives rise oligosaccharides or monosaccharides as reaction products. The enzyme strictly recognizes uronic acids scattered on the polysaccharide molecules and splits the polysaccharides at the acid site through β-elimination reaction. However, these enzymes have no similarities in their primary structures, thus suggesting that the function of these enzymes are written by different amino acid sequences. To elucidate the reason why the enzymes with the different primary structures could show the same substrate recognition and β-elimination reaction, the X-ray crystal structures of Sphingomonas sp. A1 alginate lyase (A1-III), Pseudomonas aeruginosa PAO1 alginate lyase (PA1167), and Bacillus sp.GL1 xanthan lyase were determined and compared with those of porcine kidney N-acyl-D-glucoamine 2-epimerase and Bacillus … More sp.GL1 unsaturated glucuronylhydrolase. The structural comparison of these enzymes indicated that (1)A1-III expresses β-eliination activity by using a lid-loop module. Such the structure is also found in XL, although PA1167 has not. (2) A1-III has α/α -barell structure. PA1167 shows anti-parallel β-sheet structure. XL shows a combined structure of α/α -barrell structure and anti-paralell β-sheet structure. (3)In all the polysaccharide lyases examined, Tyr is an essential catalytic amino acid residue, and His and Arg are involved in the recognition and stabilization of substrate. From these results, it was concluded that, even though the primary structure is different, similar structure for catalytic site and substrate recognition are created through the movement of protein modules. This notion indicates that the estimation of protein function on the basis of primary structure is not always reliable and definitive function of proteins should be determined in combination with the three-dimensional structures of them. Less
多糖裂解酶是催化多糖如藻酸盐、黄原胶、结冷胶和透明质酸盐解聚的酶,所有这些多糖都由细菌产生,并产生寡糖或单糖作为反应产物。该酶严格识别分散在多糖分子上的糖醛酸,并通过β-消除反应在酸性位点裂解多糖。然而,这些酶在它们的一级结构中没有相似性,因此表明这些酶的功能由不同的氨基酸序列编写。为了阐明不同一级结构的酶具有相同底物识别和β-消除反应的原因,测定了鞘氨醇单胞菌A1藻酸盐裂解酶(A1-III)、铜绿假单胞菌PAO 1藻酸盐裂解酶(PA 1167)和芽孢杆菌GL 1黄原胶裂解酶的X-射线晶体结构,并与猪肾N-酰基-D-葡糖胺2-差向异构酶和芽孢杆菌GL 1黄原胶裂解酶的晶体结构进行了比较 ...更多信息 sp.GL1不饱和葡萄糖醛酸水解酶。这些酶的结构比较表明:(1)A1-III通过使用盖环模块表达β-消除活性。这种结构也在XL中发现,尽管PA 1167没有。(2)A1-III为α/α -barell结构。PA 1167显示反平行β折叠结构。XL为α/α -barrell结构和反胶束β-折叠结构的复合结构。(3)In在所研究的所有多糖裂解酶中,Tyr是必需的催化氨基酸残基,His和Arg参与底物的识别和稳定。从这些结果可以得出结论,即使一级结构不同,通过蛋白质模块的移动,也会产生催化位点和底物识别的相似结构。这一概念表明,蛋白质功能的一级结构的基础上估计并不总是可靠的,并确定蛋白质的功能,应结合它们的三维结构来确定。少

项目成果

期刊论文数量(56)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Osamu Miyake: "An exotype alginate lyase in Sphingomonas sp. A1 : overexpression in Escherichiacoli, purification, and characterization of alginate lyase IV(A1-IV)"Protein Expression and Purification. 29(1). 33-41 (2003)
Osamu Miyake:“鞘氨醇单胞菌 A1 中的外型藻酸盐裂合酶:在大肠杆菌中过度表达、藻酸盐裂合酶 IV(A1-IV) 的纯化和表征”蛋白质表达和纯化。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Masayuki Yamasaki: "Crystallization and preliminary X-ray analysis of alginate lyase, a member of polysaccharide lyase family PL-7, from Pseudomonas aeruginosa"Acta Crystallographica setion D. 59. 1499-1501 (2003)
Masayuki Yamasaki:“来自铜绿假单胞菌的藻酸盐裂解酶(多糖裂解酶家族 PL-7 的成员)的结晶和初步 X 射线分析”Acta Crystallographa 第 D. 59. 1499-1501 (2003)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
橋本 渉: "細菌『超チャネル』の構造と高分子輸送機能"生化学. 74(7). 563-567 (2002)
Wataru Hashimoto:“细菌‘超级通道’的结构和大分子转运功能”,生物化学 74(7) (2002)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Structure and Function of a Hypothetical Pseudomonas aeruginosa Protein PA1167 Classified into Family PL-7
  • DOI:
    10.1074/jbc.m402466200
  • 发表时间:
    2004-07
  • 期刊:
  • 影响因子:
    4.8
  • 作者:
    M. Yamasaki;Satoko Moriwaki;O. Miyake;W. Hashimoto;K. Murata;B. Mikami
  • 通讯作者:
    M. Yamasaki;Satoko Moriwaki;O. Miyake;W. Hashimoto;K. Murata;B. Mikami
Keiko Momma: "Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0Å resolution"Journal of Molecular Biology. 316(5). 1051-1059 (2002)
Keiko Momma:“AlgQ2 的晶体结构,一种鞘氨醇单胞菌 A1 的大分子(藻酸盐)结合蛋白,分辨率为 2.0Å”《分子生物学杂志》316(5) (2002)。
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  • 影响因子:
    0
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MURATA Kousaku其他文献

MURATA Kousaku的其他文献

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{{ truncateString('MURATA Kousaku', 18)}}的其他基金

Infection mechanism of nitrogen-fixing bacteria to nonlegume and its application to construction of agriculture independent on chemical nitrogenous fertilizers
固氮菌对非豆科植物的侵染机制及其在建设非化学氮肥农业中的应用
  • 批准号:
    24658076
  • 财政年份:
    2012
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Structure and function of complex of substrate-binding protein and macromolecule-specific ABC transpoter
底物结合蛋白与大分子特异性ABC转座子复合物的结构与功能
  • 批准号:
    23380049
  • 财政年份:
    2011
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular identification of nitrogen-transporter and analysis of nitrogen-fixing reaction in bacteria
细菌氮转运蛋白的分子鉴定及固氮反应分析
  • 批准号:
    22658026
  • 财政年份:
    2010
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Structure/function relationship and cell surface localization mechanism of bacterial flagelin
细菌鞭毛蛋白的结构/功能关系及细胞表面定位机制
  • 批准号:
    20380049
  • 财政年份:
    2008
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular basis for cell surface architecture and evolution of bacterial flagellin by structure and unction analysis of the protein
通过蛋白质的结构和功能分析,了解细胞表面结构和细菌鞭毛蛋白进化的分子基础
  • 批准号:
    17380053
  • 财政年份:
    2005
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Structure/function analysis of abundance and transmission mechanism of genetic information invlolved in the biosynthesis of multi-catalytic site enzyme
多催化位点酶生物合成涉及的遗传信息丰度及传递机制的结构/功能分析
  • 批准号:
    11460039
  • 财政年份:
    1999
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function and safety of bacterial alginate lyase for the therapy of biofilm-dependent infection of Pseudomonas aeruginosa
细菌藻酸盐裂解酶治疗铜绿假单胞菌生物膜依赖性感染的功能和安全性
  • 批准号:
    10556017
  • 财政年份:
    1998
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
X-ray diffraction study and reaction mechanism of bacterial alginate lyase
细菌海藻酸裂解酶的X射线衍射研究及反应机制
  • 批准号:
    07660111
  • 财政年份:
    1995
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Safety assessment of genetically engineered yeast and breeding of non-toxigenic industrially used yeasts
基因工程酵母的安全性评价及无毒工业用酵母的选育
  • 批准号:
    07556091
  • 财政年份:
    1995
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Genetic and enzymatic analyses of C-P bond cleavage enzyme in bacteria
细菌 C-P 键裂解酶的遗传和酶学分析
  • 批准号:
    03660113
  • 财政年份:
    1991
  • 资助金额:
    $ 8.51万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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