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A study on PP cells in the chicken pancreas

鸡胰腺PP细胞的研究

基本信息

批准号：
62560265
负责人：
WATANABE Tohru
金额：
$ 1.15万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

A peptide, referred to as avian pancreatic polypeptide(APP), has been isolated from the pancreas of chicken. By STERNBERGER's peroxidaseantiperoxidase method using a specific antiserum against app chicken pancreas cxamined. APP cells were also studied by the immunohistochemistry after fluorescence microscopy by FALCK-HILLARP's method.The immunoreactivity was localized to a population of endocrine cells, distinct from the A, B and D cells. Pancreatic polypeptide(PP) cells were numerous and disseminated in the exocrine parenchyma. It was found that the head region contained the greatest number of PP cells with the body next and the tail having the smallest number. PP cells of the head and body regions were concentrated in the closest to the proximal duodenum. Only a few PP cells were localized in the dorsal part of ventral lobe and in the splenic lobe where the glucagon-containing a cells occurred preferentially. PP cells were polygonal or pyramid-shaped and had elongated processes among the exocrine cells, and processes were associated with capillaries.No PP cells were found in the A and B islets. D cells were distributed peripherally in A and B islets and were dispersed in the exocrine tissue. PP cells were roughly as numerous in the exocrine parenchyma as D cells. In no case did the two peptide-immunoreactivity localized in the same cells. No formaldehyde-induced fluorescence was detected in the PP cells. But PP cells exhibited immunohistochemical reactions with anti-serotonin and aromatic laminoacid decarboxylase(AADC) sera. No PP cells showed immunoreactivity for antisera against catecholamine synthesizing enzymes and some kinds of neuropeptides.AChE-positive nerve fibers innervated the exocrine parenchyma, the intercalated ductular cells and the smooth muscles of blood vessels. There is no evidence to show that PP cells were richly innervated with AChE-positive nerve fibers.

从鸡的胰腺中分离出一种多肽，称为禽胰多肽(APP)。用抗APP鸡胰腺的特异性抗血清，用Sternberger‘s过氧化物酶-抗过氧化物酶方法检测APP鸡胰腺的抗氧化物酶活性。用Falck-Hillarp方法对APP细胞进行荧光显微镜下的免疫组织化学染色，免疫反应定位于一组不同于A、B、D细胞的内分泌细胞。胰腺多肽(PP)细胞较多，散在分布于外分泌实质。结果发现，头部含有最多的PP细胞，体部次之，尾部最少。头、体部PP细胞主要集中在十二指肠近端。仅少数PP细胞定位于腹叶背侧和脾叶，其中以含胰高血糖素的a细胞为主。PP细胞呈多边形或金字塔状，外分泌细胞间有细长突起，突起与毛细血管相连，A、B两个胰岛未见PP细胞。D细胞分布于A、B胰岛周缘，散在分布于外分泌组织。外分泌实质中PP细胞与D细胞大致相同。在任何情况下，这两种多肽免疫反应都没有定位在同一细胞中。在PP细胞中未检测到甲醛诱导的荧光。PP细胞与抗5-羟色胺和芳香族氨基酸脱羧酶(AADC)血清呈免疫组织化学反应。未见PP细胞对儿茶酚胺合成酶和某些神经肽的抗血清呈免疫反应。AChE阳性神经纤维分布于外分泌实质、间质导管细胞和血管平滑肌。没有证据表明PP细胞内有丰富的AChE阳性神经纤维支配。