A study on PP cells in the chicken pancreas

从鸡胰腺中分离到禽胰多肽（APP）。采用STERNBERGER过氧化物抗过氧化物酶法对鸡胰腺进行特异性抗血清检测。荧光显微镜下应用FALCK-HILLARP法对APP细胞进行免疫组化研究。免疫反应性定位于内分泌细胞群，不同于a、B和D细胞。胰腺多肽（PP）细胞大量分布于外分泌实质。结果表明，PP细胞数量以头部最多，其次为体，尾部最少。头、体PP细胞集中于最靠近十二指肠近端。只有少量PP细胞定位于腹叶背侧和脾叶，脾叶中含有胰高血糖素的细胞优先出现。PP细胞呈多角形或锥体状，外分泌细胞间突起较长，突起与毛细血管相关。A、B胰岛未见PP细胞。D细胞外周分布于A、B胰岛，分散于外分泌组织。外分泌薄壁组织中PP细胞的数量与D细胞大致相同。在任何情况下，这两种肽免疫反应性都不局限于同一细胞。PP细胞未检测到甲醛诱导的荧光。但PP细胞对抗血清素和芳香胺酸脱羧酶（AADC）血清表现出免疫组化反应。未发现PP细胞对儿茶酚胺合成酶和某些神经肽的抗血清具有免疫反应性。痛觉阳性神经纤维支配外分泌薄壁组织、间插导管细胞和血管平滑肌。没有证据表明PP细胞被丰富的疼痛阳性神经纤维支配。

A peptide, referred to as avian pancreatic polypeptide(APP), has been isolated from the pancreas of chicken. By STERNBERGER's peroxidaseantiperoxidase method using a specific antiserum against app chicken pancreas cxamined. APP cells were also studied by the immunohistochemistry after fluorescence microscopy by FALCK-HILLARP's method.The immunoreactivity was localized to a population of endocrine cells, distinct from the A, B and D cells. Pancreatic polypeptide(PP) cells were numerous and disseminated in the exocrine parenchyma. It was found that the head region contained the greatest number of PP cells with the body next and the tail having the smallest number. PP cells of the head and body regions were concentrated in the closest to the proximal duodenum. Only a few PP cells were localized in the dorsal part of ventral lobe and in the splenic lobe where the glucagon-containing a cells occurred preferentially. PP cells were polygonal or pyramid-shaped and had elongated processes among the exocrine cells, and processes were associated with capillaries.No PP cells were found in the A and B islets. D cells were distributed peripherally in A and B islets and were dispersed in the exocrine tissue. PP cells were roughly as numerous in the exocrine parenchyma as D cells. In no case did the two peptide-immunoreactivity localized in the same cells. No formaldehyde-induced fluorescence was detected in the PP cells. But PP cells exhibited immunohistochemical reactions with anti-serotonin and aromatic laminoacid decarboxylase(AADC) sera. No PP cells showed immunoreactivity for antisera against catecholamine synthesizing enzymes and some kinds of neuropeptides.AChE-positive nerve fibers innervated the exocrine parenchyma, the intercalated ductular cells and the smooth muscles of blood vessels. There is no evidence to show that PP cells were richly innervated with AChE-positive nerve fibers.