权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Electron Microscope Autoradiographic Study on Brain Gut Peptide Uptake by Platelets

血小板摄取脑肠肽的电子显微镜放射自显影研究

基本信息

批准号：
62570018
负责人：
DAIMON Tateo
金额：
$ 0.38万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Multiple Morphological, Biochemical and Pharmacological Similarities Exist Between Blood Platelets and Paraneurons Which are Cells Generally Have not Been Counted as Neurons and Yet can be Regarded as Related to Neurons. It Should be Expected That Platelets Would be Able to Store Neuropeptides, Since The Coexistence of Multiple Amines and Brain Gut Peptides in The Paraneuron has Been Demonstrated. To Verify This Hypothesis, we Examined the Uptake and Storage of ^3H-Labeled Brain Gut Peptides by Rabbit Platelets and Megakaryocytes in Vitro Using Autoradiographic Techniques. The Following Radiochemicals Were Used : ^3H-Angiotensin II, ^3H-Neuropeptide Y, ^3H-Vasopressin and ^3H-Substance P. Suspensions of Platelets in Plasma and Bone Marrow Tissues in Eagle's Medium Were Incubated With 5 Micro CI of Each Isotope for 60 Min at 37ﾟ C. A Light Microscopic Autoradiography has Revealed The Active Uptake of ^3H-Angiotensin II by Megakaryocytes. ^3H-Angiotensin II Uptake by Platelets Has Also Confirmed by Electron Microscopic Autoradiography. Silver Grains Are Preferentially Associated With Dense Bodies of Platelets Which are Also the Storage Sites of Catecholamines. Neither Megakaryocytes Nor Platelets Show any Active Uptake of ^3H-Neuropeptide Y and ^3H-Substance P. Megakaryocytes are Slightly Labeled After Incubation with ^3H-Vasopressin. Autoradiographic Analysis Has Also Performed to Clarify Whether or not Submammalian Thrombocytes can Take up in Vitro ^3H-Agniotensin II. The Uptake of the Isotope is Confirmed in Chicken, but not in Frog.

血小板和副神经元之间存在着多种形态学、生物化学和药理学上的相似性，它们是通常不被算作神经元的细胞，但可以被认为与神经元有关。由于已经证实了多种胺和脑肠肽在血小板中的共存，因此应该预期血小板能够储存神经肽。为了验证这一假设，我们在体外用放射自显影技术检测了兔血小板和巨核细胞对^3H标记的脑肠肽的摄取和储存。使用了以下放射性化学物质：^3 H-血管紧张素II、^3 H-神经肽Y、^3 H-加压素和^3 H-P物质。将血浆和骨髓组织中的血小板悬浮液置于Eagle培养基中，与每种同位素的5微CI一起在37 ° C下孵育60分钟。光镜放射自显影显示巨核细胞对^3H-血管紧张素II的主动摄取。电子显微镜放射自显影也证实了血小板对^3 H-血管紧张素II的摄取。银颗粒与血小板的致密体有关，血小板也是儿茶酚胺的储存场所。巨核细胞和血小板都没有表现出对^3 H-神经肽Y和^3 H-P物质的任何主动吸收。巨核细胞在与^3 H-加压素孵育后有轻微标记。还进行了放射自显影分析，以阐明亚哺乳动物血小板是否能在体外摄取^3 H-腺苷降压素II。同位素的摄取在鸡中得到证实，但在青蛙中没有。