Mechanisms of the storage of metalic elements in amine granules during granulation studied by rapid freezing

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The mechanisms and the processes of the storage of metalic elements in the amine graules of megakaryocytes and chromaffin cells in the adrenal medulla were studied by cryoultramicroscopy and X-ray microprobe analysis.1. Blood platelets and megakaryocytes of miceX-ray microprobe analysis (EDX) of freeze-substituted platelets demonstrated that amine storage granules contained Mg, Ca and P.However, the precursors of the amine storage granules in megakaryocytes, the mother cells of platelets, contained Mg, Ca and P.Since quantitative differences in Mg and Ca of the granules were not observed between megakaryocytes and platelets, megakaryocytes readily accumulate cations and P (ATP) in the precursors of the amine storage organelles in which serotonin is not stored yet.2. Chromaffin cells in the adrenal medulla of ratsX-ray microanalysis of freeze-substituted adrenal medulla revealed large amounts of Ca, K,P in noradrenaline granules, whereas adrenaline granules contained K and P.Ca in the adrenaline granules was barely detectable.