Mechanisms of the storage of metalic elements in amine granules during granulation studied by rapid freezing

快速冷冻造粒过程中胺颗粒中金属元素的储存机制研究

• 批准号: 04670027
• 负责人: DAIMON Tateo
• 金额: $ 1.28万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670027/
• 关键词: Rapid freezing; X-ray analysis; Electron microscope; Blood platelet; Adrenal medulla; Amine granules; Granulation; Metalic element; 巨核球; アミン貯蔵元素; 骨髄巨核球; 急速凍結法; 分析電子顕微鏡; 副腎髓質

The mechanisms and the processes of the storage of metalic elements in the amine graules of megakaryocytes and chromaffin cells in the adrenal medulla were studied by cryoultramicroscopy and X-ray microprobe analysis.1. Blood platelets and megakaryocytes of miceX-ray microprobe analysis (EDX) of freeze-substituted platelets demonstrated that amine storage granules contained Mg, Ca and P.However, the precursors of the amine storage granules in megakaryocytes, the mother cells of platelets, contained Mg, Ca and P.Since quantitative differences in Mg and Ca of the granules were not observed between megakaryocytes and platelets, megakaryocytes readily accumulate cations and P (ATP) in the precursors of the amine storage organelles in which serotonin is not stored yet.2. Chromaffin cells in the adrenal medulla of ratsX-ray microanalysis of freeze-substituted adrenal medulla revealed large amounts of Ca, K,P in noradrenaline granules, whereas adrenaline granules contained K and P.Ca in the adrenaline granules was barely detectable.

用冰冻显微镜和X射线微探针分析方法研究了大鼠肾上腺髓质巨核细胞和嗜铬细胞的胺颗粒中金属元素的储存机制和过程。结果：1.冷冻替代血小板的血液和巨核细胞的显微探针分析表明，胺储存颗粒中含有镁、钙和磷，而巨核细胞中的胺储存颗粒的前体含有镁、钙和磷。由于巨核细胞和血小板中的镁和钙的数量没有差异，巨核细胞很容易在未储存5-羟色胺的胺储存细胞器的前体中积聚阳离子和P(ATP)。大鼠肾上腺髓质嗜铬细胞冷冻替代肾上腺髓质的X射线显微分析显示去甲肾上腺素颗粒中含有大量的钙、钾、磷，而肾上腺素颗粒中含有钾和磷，几乎检测不到钙。

项目成果

Daimon,T.: "Cranulation in amine-storage organelles of mouse megakaryocytes:X-ray microprobe analysis and radioautography" Cellular and Molecular Biology. 41. 87-95 (1995)

Daimon,T.：“小鼠巨核细胞胺储存细胞器的造血：X 射线微探针分析和放射自显影”细胞和分子生物学。

T.Daimoin,K.Kawai,K.Uchida: "Granulation in amine-storage organelles of mouse megakaryocytes:X-raymicroprobe analysis and radioautography" 40. 未定 (1994)

T.Daimoin、K.Kawai、K.Uchida：“小鼠巨核细胞胺储存细胞器中的颗粒化：X 射线微探针分析和放射自显影”40。待定（1994 年）

T.Daimoin,K.Kawai,K.Uchida: "Granulation in amine-storage organelles of mouse megakaryocytes:X-raymicroprobe analysis and radioautography" 40. 未定 (1994)ellular and Molecular Bioloogy.

T.Daimoin、K.Kawai、K.Uchida：“小鼠巨核细胞的胺储存细胞器中的颗粒化：X 射线微探针分析和放射自显影”40。待确定（1994）细胞和分子生物学。

Daimon,T.: "Granulation in amine-storage organelles of mouse megakaryocytes:X-ray microprobe analysis and radioautography" Cellular and Molecular Biology. 41. 87-95 (1995)

Daimon,T.：“小鼠巨核细胞胺储存细胞器的颗粒化：X 射线微探针分析和放射自显影”细胞和分子生物学。

Daimon, Tateo: "Granulation in amine-storage organelles of mouse megakaryocytes : X-ray microprobe analysis and radioautography" Cellular and Molecular Biology. 41-1. 87-95 (1995)

Daimon，Tateo：“小鼠巨核细胞胺储存细胞器的颗粒化：X 射线微探针分析和放射自显影”细胞和分子生物学。