ゼノパス胚胸腺原基への胸腺リンパ球幹細胞流入に関する実験発生学的研究

胸腺淋巴细胞干细胞流入非洲爪蟾胚胎胸腺原基的实验胚胎学研究

• 批准号: 62570014
• 负责人: 栗原 一茂
• 金额: $ 0.77万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570014/
• 关键词: 胸腺; Tリンパ球幹細胞; aging; キメラ; パラバイオシス; ゼノパス

Xenopus laevisとX.borealisの2種のカエル胚を用い種々のキメラ胚作製もしくはパラバイオシスを行う事により, Tリンパ球幹細胞の胸腺原基への流入時期, 流入について幹細胞と胸腺原基との関連について調べた. (1)22時間胚で前部がX.1後部がX.bとなるキメラ胚を作り一定時間後心臓原基を除去して5日後の胸腺原基を調べ, 受精後3日9〜12時間に幹細胞が流入する事を確認した. (2)発生段階(stage)が12時間異なる2者の胚を用い, (1)と同様のキメラ胚を作製し5日後胸腺原基を調べてみると, 大半のキメラ胚で, 後部由来の細胞の胸腺原基への流入は認められなかった. このキメラ胚においては幹細胞と胸腺原基との間に12時間のagingの差があり, 幹細胞の流入にはそれ自身, もしくは胸腺原基のagingが関与している事が強く示唆された. この事を更に調査するために(3)stageの異なる(0〜24時間差)2者のカエル胚(young embryo,YE=X.laevis,old,OE=X.borealis)を用いパサバイオシスを行った. これによって流入現象と, 幹細胞と胸腺原基のagingとの関連が調査しうる. 同じstageの胚を用いたパラバイオシスでは受精後2日の時期に両者に血球の混在が認められ, 互いの血管系の連絡が確認された. 5日後の両者の胸腺原基には互いの細胞が存在し, この系において2者の細胞間の親和性の違いは無視しうると考えられた. この相互の流入はstageの差が3〜4時間のseriesでも認められた. しかし差が6時間のseriesになると, OE側胸腺原基にYE側の細胞は認められるが, YE側の胸腺原基へOE側の細胞は流入しない. この関係はstageの差が10, 13, 16, 24時間のseriesにおいても同様であった. この事は正常発生において受精後3日9〜12時間で胸腺原基へ幹細胞が流入しはじめるが, 6時間以内に流入が停止する可能性が示唆される. それは胸腺原基ではなく, 流入する幹細胞自身のagingに関与していると考えられる.

Xenopus laevis X.borealis two kinds of mouse embryos were used to conduct business operations, and the thymic primordia of T cells were inflowed during the period of inflow, and then flowed into the thymic primordia of the mouse cells. (1) after 22 hours, the anterior part of the embryo X.1, the back part of the embryo X.b., after the removal of the primordial embryo for a certain period of time, the primordial embryo of the thymus was removed for a certain period of time, and at 9: 12 on the 3rd day after fertilization, the cell was transferred into the cell for confirmation. (2) when stage was 12 hours old, 2 embryos were used. (1) after 5 days, the primordial embryo of the thymus was used, and most of the embryos were aborted. The latter part of the embryo flowed into the embryo of the mother. The aging of the thymic primordia was not detected during the 12-hour period of time, and the thymic primordium cells flowed into the cell itself. The thymic primordia aging cells and the thymic primordia were forced to indicate that they were instigated. If you need to change your schedule, please check (3) if you have a stage error (024 hours), if you have an error (young embryo,YE=X.laevis,old,OE=X.borealis), you will need to make sure that you do not need to do so. The cells flow into the elephant, and the thymus primordium aging cells inflow the thymus. With the same stage embryo, the blood cells were mixed with those who were fertilized at some time after 2 days of fertilization, and the vascular system connection was confirmed by each other. After 5 days, the thymic primordia were isolated from each other, and the cells of the thymic primordia were isolated from each other. Each other flows into the stage after a difference of 3: 4 per cent of the time, and the series flows into each other in a short time. After 6 minutes, the series primordial OE cells of OE thymus and YE thymic progenitor cells were incubated, and the cells of primordial thymus cells of YE thymus cells flowed into the trophoblast. Make sure that the stage difference is 10, 13, 16, 24 and the series is the same as the other. After fertilisation, the thymic primordium cells of normal pregnant women flowed into the pregnant women within 9: 12 hours on the 3rd day, and the possibility of the inflow of thymic primordial cells into the pregnant women within 6 days indicated that they were instigated. The primordia of the thymus flow into the cells of the thymus, which inflow the cells of the thymus, aging cells and the cells themselves.