Establishment of animal model for gastro-enteritis induced by Campylobacter pylori using germfree animals

本课题旨在建立幽门弯曲杆菌（c. pylori）诱发的胃肠炎动物模型，并观察其免疫应答。无菌动物胃肠道粘膜淋巴组织发育不全，绒毛上皮几乎不表达ia抗原。经微生物处理后，高凝血酶迅速发展，IgA浆细胞和T淋巴细胞数量显著增加。绒毛上皮中出现ia抗原。男性胃黏膜幽门螺旋杆菌阳性，小窝上皮增生，IgA、IgG浆细胞及白细胞明显增多。幽门螺旋杆菌感染的粘膜T淋巴细胞数量没有增加。提示幽门螺旋杆菌感染可引起胃粘膜的体液免疫反应和炎症反应，并引起胃粘膜中央凹腺的增生性改变。这些结果可能为阐明胃炎和溃疡性疾病的机制提供重要线索。

The aim of this research project was to establish an animal model for gastroenteritis induced by Campylobacter pyloridis (c. pylori), and to observe the immune responses to these organisms.In germfree animals, the lymphoid tissue in gastro-intestinal mucosa (GALT) was poorly developed, and the expression of Ia-antigens by villous epithelium was almost absent. By the administration of the microorganisms, galt was rapidly developed, and numbers of IgA plasma cells and T lymphocytes were much increased. Ia-antigens appeared in the villous epithelium.In man, gastric mucosa positive for C. pylori, hyperplasia of the foveolar epithelium and remarkable increase of IgA and IgG plasma cells and leucocytes were observed. The number of T lymphocytes in the C. pylori-infected mucosa did not grow larger. This suggests that the C. pylori-infection induce humoral immune responses and inflammation, and proliferative changes of the foveolar gland in the gastric mucosa.These results may give an important clue to elucidate the mechanism of inflammatory and ulcerative diseases in the stomach.