Studies on age-related changes in senescence accelerated (SAM-P) mice.

加速衰老（SAM-P）小鼠的年龄相关变化的研究。

• 批准号: 62570160
• 负责人: MORI Kazuhiro
• 金额: $ 0.96万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570160/
• 关键词: Ageing; Senescence accelerated mice; Hemopoiesis; Hemopoietic stem cell; 白血球前駆細胞; SAM-Pマウス(Senescence Accelerated Mouse)

Senescence accelerated mice (SAM-P) were established by Prof. T. Takeda at Kyoto University.This study concerns age-related changes in hemopoietic system of SAM-P.Numbers of hemopoietic stem cells (CFU-S) and granulocyte-macrophage colony-forming cells (GM-CFC) showed a significant decrease in old mice compared to those in young mice. Reportedly non-accelerated mice of the same strain SAM-R), however, also showed an age-related decrease in hemopoietic activity. This forced us to use normal mice of other strains.No significant changes were observed in normal mice of BDFI and ddy strains in the numbers of hemopoietic stem cells and GM-CFC.A possibility of age-related decrease in the number of mast cell-progenitors is also under study, since mast cell-progenitors appear to be closely related to CFU-S and since we have establishe an in vitro assay system of the progenitors with a high seeding efficiency which facilitates quantitative evaluation of mast cell progenitor.

衰老加速小鼠（SAM-P）是由京都大学 T. Takeda 教授建立的。这项研究关注 SAM-P 造血系统与年龄相关的变化。与年轻小鼠相比，老年小鼠的造血干细胞（CFU-S）和粒细胞-巨噬细胞集落形成细胞（GM-CFC）数量显着减少。然而，据报道，同一品系 SAM-R 的非加速小鼠也表现出与年龄相关的造血活性下降。这迫使我们使用其他品系的正常小鼠。在 BDFI 和 ddy 品系的正常小鼠中，没有观察到造血干细胞和 GM-CFC 数量的显着变化。肥大细胞祖细胞数量与年龄相关的减少的可能性也在研究中，因为肥大细胞祖细胞似乎与 CFU-S 密切相关，而且我们已经建立了祖细胞的体外测定系统 高接种效率，有利于肥大细胞祖细胞的定量评估。

项目成果

Itoh,Katuhiko: Exp.Hematol.17. 145-153 (1989)

伊藤，克彦：Exp.Hematol.17。

Tsuruwawa, Masahito: Assay of colony promoting activity (CPA).Tokyo Kagaku Dojin-Sha, (Tokyo Association of Chemists)., 31-35 (1987)

Tsuruwawa, Masahito：集落促进活性 (CPA) 的测定。Tokyo Kagaku Dojin-Sha，（东京化学家协会）。，31-35 (1987)

Sawada,Hitoshi: Leuk.Res.12. 763-771 (1988)

泽田仁：Leuk.Res.12。

Kirikae, Teruo: Acta Haemotol.JPX. 50. 784-790 (1987)

Kirikae，Teruo：Acta Haemotol.JPX。

Sakoda,Hiroto: Exp.Hematol.,. (1989)

迫田弘人：Exp.Hematol.，。