Studies on age-related changes in senescence accelerated (SAM-P) mice.

衰老加速小鼠(SAM-P)是由京都大学武田教授建立的。本研究探讨了衰老加速小鼠造血系统的增龄性变化。结果表明，老龄小鼠的造血干细胞(CFU-S)和粒细胞-巨噬细胞集落形成细胞(GM-CFCs)数量明显低于青年小鼠。然而，据报道，相同品系的非加速小鼠(SAM-R)也显示出与年龄相关的造血活性下降。这迫使我们使用其他品系的正常小鼠。在BDFI和Ddy品系的正常小鼠中，造血干细胞和GM-CFC的数量没有明显变化。肥大细胞前体细胞数量随年龄的减少的可能性也在研究中，因为肥大细胞前体细胞似乎与CFU-S密切相关，而且我们已经建立了一套种子效率高的肥大细胞前体细胞体外检测系统，便于对肥大细胞前体细胞的定量评估。

Senescence accelerated mice (SAM-P) were established by Prof. T. Takeda at Kyoto University.This study concerns age-related changes in hemopoietic system of SAM-P.Numbers of hemopoietic stem cells (CFU-S) and granulocyte-macrophage colony-forming cells (GM-CFC) showed a significant decrease in old mice compared to those in young mice. Reportedly non-accelerated mice of the same strain SAM-R), however, also showed an age-related decrease in hemopoietic activity. This forced us to use normal mice of other strains.No significant changes were observed in normal mice of BDFI and ddy strains in the numbers of hemopoietic stem cells and GM-CFC.A possibility of age-related decrease in the number of mast cell-progenitors is also under study, since mast cell-progenitors appear to be closely related to CFU-S and since we have establishe an in vitro assay system of the progenitors with a high seeding efficiency which facilitates quantitative evaluation of mast cell progenitor.