Genetic and Biochemical Studies on H, Se, and X genes encoded fucosyltransferases responsible for biosynthesis of blood group antigens.

对血型物质中负责合成岩藻糖缀合物的-岩藻糖基转移酶进行检测和表征，以阐明-岩藻糖基转移酶与岩藻糖基血型物质之间的关系，岩藻糖基血型物质是基因如H、Se、Le和X.1）利用新的化学合成的寡糖来测定糖基转移酶的特异性可以提供特异性的测定系统用于（12）-、（13）-、（14）-L-岩藻糖基转移酶、N-乙酰基-D-葡糖胺基转移酶和唾液酸转移酶。2)本文用抗Le ^a和抗Le ^B单克隆抗体建立了一种简便的放射免疫分析法，用于测定人血清和唾液中的刘易斯抗原。使用这种免疫分析方法，在新生儿、妊娠样本和癌症患者样本中证明了不相容的刘易斯血型抗原的表达。负责Le^a抗原的（14）-L-岩藻糖基转移酶

Detection and characterization of -fucosyltransferases responsible for the synthesis of fucoglycoconjugates in blood group substances were undertaken to elucidate the relationship between -fucosyltransferases and fucosyl blood group substances which were primary and secondary products of genes such as H, Se, Le and X.1) The availability of new chemically synthesized oligosaccharides for determining the specificities of glycosyltransferases could provide specific assay systems for (1 2)-, (1 3)-, (1 4)-L-fucosyltransferases, N-acetyl-D-glucosaminyltransferase and sialyltransferases. 2) A simple radioimmunoassy method was developed for the measurement of Lewis antigens in human sera and saliva with monoclonal anti-Le^a and anti-Le^b antibodies. Using this immunoassay method, the expression of incompatible lewis blood group antigens was demonstrated in new-born, pregnant samples and samples from cancer patients. (1 4)-L-Fucosyltransferase responsible for Le^a antigen