Platelet Epidermal Growth Factor and Liver Regeneration

1.建立大鼠表皮生长因子的测定方法（rEGF）： A放射性免疫测定是为rEGF开发的，它是从大鼠亚maxillary地纯化的。“碘化肽需要进一步的纯化与一个免疫亲和性列进行敏感的测定。”因此，两个敏感性和简单的两个位点酶免疫测定已经开发出使用Fab'抗体链接过氧化物酶或-D-半乳糖苷酶的抗rEGF活性，任何活性在6个月内稳定在4 ° C。人类等离子体的附加物没有受到干扰。TherEGF可在0.5 pg/tube.2中检测到。rEGF的组织分布：亚maxillary gland， seminal vesicle， prostate， pacreas和kidney在rEGF中非常丰富。二进制数字显示了更高的内容，并将其与其他部分的数据进行了比较。Portal or systemic blood specimens of several conditions （treated with several secretagogues or at several time point of liver regeneration） revealed no detectable levels of rRF。更高的放射活性因子被注射到体外受精的门户血管被累积到细胞核中，在肝脏再生早期阶段比较了那些控制。REGF对肝脏再生的直接影响已被建议。进一步的研究需要在肝脏再生中发挥平台EGF的作用（s）。

1. Establishing of assay methds of rat epidermal growth factor (rEGF): A radioimmunoassay was developed for rEGF, which was purified from rat male submaxillary glands. The iodinated peptide required further purification with a immunoaffinity column for the sensitive assay. So, two sensitive and simple two-site enzyme immunoassays were developed using anti-rEGF Fab'antibody-linked peroxidase or -D-galactosidase, whose activities were stable at 4゜C for 6 months. No interference was observed by addition of human plasma. TherEGF was detectable at a concentration of 0.5 pg/tube.2. Tissue distribution of rEGF: Submaxillary gland, seminal vesicle, prostate, pacreas, and kidney were rich in rEGF. Duodenum showed higher content compared to other segments of digestive tract. Portal or systemic blood specimens of several conditions (treated with several secretagogues or at several time points of liver regeneration) revealed no detectable levels of rERF. Higher radioactivities of iodinated rEGF injected to portal vein were accumulated in the nuclei of the hepatocytes at the early phase of liver regeneration compared to those of controls. Direct effect of rEGF to liver regeneration was suggested. Further study was needed to elucidate the role(s) of platelet EGF on liver regeneration.