Platelet Epidermal Growth Factor and Liver Regeneration

血小板表皮生长因子与肝脏再生

• 批准号: 62570313
• 负责人: IKEDA Hitoshi
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570313/
• 关键词: Liver Regeneration; Epidermal Growth Factor; Growth Factor; Platelet; イムノアッセイ

1. Establishing of assay methds of rat epidermal growth factor (rEGF): A radioimmunoassay was developed for rEGF, which was purified from rat male submaxillary glands. The iodinated peptide required further purification with a immunoaffinity column for the sensitive assay. So, two sensitive and simple two-site enzyme immunoassays were developed using anti-rEGF Fab'antibody-linked peroxidase or -D-galactosidase, whose activities were stable at 4゜C for 6 months. No interference was observed by addition of human plasma. TherEGF was detectable at a concentration of 0.5 pg/tube.2. Tissue distribution of rEGF: Submaxillary gland, seminal vesicle, prostate, pacreas, and kidney were rich in rEGF. Duodenum showed higher content compared to other segments of digestive tract. Portal or systemic blood specimens of several conditions (treated with several secretagogues or at several time points of liver regeneration) revealed no detectable levels of rERF. Higher radioactivities of iodinated rEGF injected to portal vein were accumulated in the nuclei of the hepatocytes at the early phase of liver regeneration compared to those of controls. Direct effect of rEGF to liver regeneration was suggested. Further study was needed to elucidate the role(s) of platelet EGF on liver regeneration.

1.大鼠表皮生长因子（rEGF）测定方法的建立：从雄性大鼠颌下腺中分离纯化rEGF，建立rEGF的放射免疫分析法。碘化肽需要用免疫亲和柱进一步纯化以用于灵敏测定。因此，我们利用抗rEGF Fab'抗体连接的过氧化物酶或-D-半乳糖苷酶建立了两种灵敏、简便的双位点酶免疫分析法，其活性在4 ℃下稳定6个月。加入人血浆后未观察到干扰。TherEGF在0.5pg/管的浓度下是可检测的。rEGF的组织分布：颌下腺、精囊、前列腺、胰腺和肾脏富含rEGF。十二指肠的含量高于消化道其他部分。几种情况下的门静脉或全身血液标本（用几种促分泌剂或在几个肝再生时间点处理）显示没有检测到rERF水平。与对照组相比，门静脉注射碘化rEGF在肝再生早期肝细胞核中积聚了更高的放射性。提示rEGF对肝再生有直接作用。血小板EGF在肝再生中的作用有待进一步研究。

项目成果

Kenji Sakamoto: Endocrinologia Japanica.

坂本健二：日本内分泌学。

Sakamoto,Kenji.: Endocrinologia Japonica.

坂本健二：日本内分泌学。

Oka,Yuji: Biochemistry International. 16. 941-948 (1988)

冈裕二：生物化学国际。

Yuji Oka: Biochemistry International.

冈雄二：生物化学国际。

Oka,Yuji.: Biochemistry International. 16. 941-948 (1988)

冈裕二：生物化学国际。