Serodiagnosis of syphilis by an enzyme-linked immunosorbent assay for IgG subclass antibodies against Treponema pallidum.

通过针对梅毒螺旋体 IgG 亚类抗体的酶联免疫吸附测定进行梅毒血清诊断。

• 批准号: 62570466
• 负责人: TANAKA Shinobu
• 金额: $ 1.09万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570466/
• 关键词: syphilis; IgG-subclass; 酵素抗体法(ELISA); ELISA(酵素抗体法); IgGアイソタイプ

An enzyme-linked immunosorbent assay (ELISA) system has been developed for detection and quantification of IG class and IgG subclasses of antibodies to Treponema pallidum (TP) in serum form patients with syphilis. Patients with various stages of infection were followed by periodic teets with ELISA to determine whether these antibodies might be of any use as diabnostic parameters in evaluating clinical responses of patients of treatment. Assays carried out on 301 patient sera, with 60 negative sera as controles, revealed occurrence of all anti-TP antibodies of IgM, IgA and IgG classes and IgG subclasses 1 to 4 in the patient sera.Serum leveles of anti-TP antibodies in 54 patients with primary syphilis, 30 with secondary syphilis and 18 with early latent syphilis, each stage-group classified into two subgroups : patients who had been treated for less than 3 months and those treated longer, were comparatively assessed to examine their trends in respective stages of infection as well as th … More eir responses to treatment. Levels of IgM and IgA antibodies in serum fell significantly following tratment at primary and early latent stages, and IgG subclasses of antibody also decreased in these patients. However. there were no IgG subclasses peculiar to any disease stage that predominated among the antibodies. The results, together with assay data from 99 patients with tertiary or late syphilis, practically all of whom had previously been treated, suggested that IgG subclass 1 antibody might account for the most part of antibodies long persisting after treatment.Fourteen primary, 8 secondary, 6 early latent and 4 tertiary stage patients were followed by periodic tests from pre-treatment period onwards to examine trends of their antibody responses to treatment. Of 32 patients, pre-treatment serum IgM antibody levels were within the normal limits in 8 patients. IgM antibody decreased following treatment in 62.5% of the remaining 24 cases. All patients exhibited positive serum levels of IgG subclass antibodyes prior to treatment. Of whom 71.9% responded with their decline as treatment progressed. The anti-TP IgM positivity rate was as low as 28.3% for previously treated tertiary syphilis patients, and that 3 out 4 previously untreated late syphilis patients showed decrease of IgG and IgG subclass antibodies after therapy. It is thus concluded that determination of these anti-TP antibodies of IgG subclasses is of value as a means of diagnosing for cure those syphilis patients showing normal IgM antibody level. Particularly in those with late syphilis. Less

已经开发了一种酶联免疫吸附测定（ELISA）系统，用于检测和定量针对梅毒的血清中毛毛虫（TP）抗体的Ig类和IgG亚类。具有各种感染阶段的患者之后是具有ELISA的周期性曲折，以确定这些抗体是否可以用作评估治疗患者临床反应的糖尿病参数。对301名患者血清进行的测定，具有60个负血清作为对照，显示出所有抗TP抗体的发生，IgM，IgA和IgG类别的所有抗TP抗体以及患者血清中的1至4个抗IgG子类别1至4，在54例抗二次梅毒和二级甲状腺中，抗TP抗体的甲状腺疗法水平为54个抗体，并在54个抗体中，均具有二次Syphilis，并以二级序列为18亚组：接受治疗少于3个月并且治疗更长的患者接受了比较评估，以检查其在感染的相对阶段以及对治疗的更多EIR反应中的趋势。在原发性和早期潜在阶段，tratement后血清中的IgM和IgA抗体水平显着下降，这些患者的IgG抗体亚类也有所改善。然而。在抗体中占主导地位的任何疾病阶段都没有IgG亚类。结果以及来自99例三级或晚期辅助患者的测定数据，几乎所有这些患者以前都接受过治疗，这表明IgG亚类1抗体可能是抗体的大部分抗体，抗体长期持续持续。小学后，8个次要，6个中期和4个潜在和4个潜在和4个第三阶段的患者在经过治疗的情况下进行了经过治疗，以进行预锻炼。在32例患者中，治疗前血清IgM抗体水平在8例患者的正常限制之内。在其余24例中62.5％的治疗后，IgM抗体在治疗后开发。所有患者在治疗前暴露了IgG亚类抗体的阳性血清水平。随着治疗的进展，其中有71.9％的人对他们的下降做出了反应。先前治疗的第三次梅毒患者的抗TP IgM阳性率高达28.3％，而治疗后的3个先前未经治疗的晚期梅毒患者的3岁抗体显示IgG和IgG亚类抗体的降低。因此，得出的结论是，确定IgG亚类的这些抗TP抗体是有价值的，作为治愈那些表现出正常IgM抗体水平的梅毒患者的诊断手段。特别是在晚期梅毒的人中。较少的

项目成果

S.Tanaka.;T.Suzuki: Sex.Transmitt.Dis.

S.Tanaka.；T.Suzuki：性传播。Dis。

T.Suzuki.;S.Tanaka: Sex.Transmitt.Dis.

T.Suzuki.；S.Tanaka：性.传输.Dis。

T.Suzuki.;S.Tanaka.: Sex.Transmitt.Dis.

T.Suzuki.；S.Tanaka.：性传播Dis。

T.Suzuki; S.Tanaka.: "Ig class- and IgG subclass antibody reactivity to antigens of Treponema pallidum after treatment of syphilis." Sex.Transmitt.Dis.

铃木T.

S.Tanaka,;T.Suzuki.: Sex.Transmitt.Dis.

S.Tanaka,;T.Suzuki.: 性别.传输.Dis.