Antigenic Analysis of Treponema Pallidum : Utilization of the TP Antigen(s) in Disease Staging as well as in Evaluating the Result of Treatment.

梅毒螺旋体的抗原分析：利用 TP 抗原进行疾病分期以及评估治疗结果。

• 批准号: 01570581
• 负责人: TANAKA Shinobu
• 金额: $ 1.34万
• 依托单位: Tokyo Metropolitan Institute of Medical Science.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570581/
• 关键词: Syphilis; Westernーblotting; IgG subclass

Changes in reactivities of Ig class and IgG subclass of anti-Treponema pallidum antibodies after treatment in sera from patients with clinical syphilis were assessed by sodium dodecyl sulfateーpolyacrylamide gel electrophoresis immunoblotting. A marked decrease in reactivity of IgM antibodies was noted after treatment and IgM antibody proved to be effective as a marker for evaluating the result of treatment. In occasional cases, however, IgM reactivity remained below the lower limit of sensitivity of the assay or failed to decline despite treatment. IgA antibodies decreased after treatment in limited cases of primary and secondary syphilis. No significant fall of IgG antibody was observed after treatment in the majority of syphilitic patients. Assessment made at the levels of IgG subclass antibodies demonstrated a decline in reactivity of one or more IgG subclass antibodies after treatment. The IgG subclasses and types of T. pallidum antigen(s) reacting with them that responded in conjunction with treatment, nevertheless, varied among patients. However, 56Kd antigen proved to be the most reliable marker antigen for the assessment of IgM reactivity. These results indicated that it is useful in evaluating the effect of treatment to monitor IgA and IgG subclass antibodies in those cases where IgM antibody fails to serve as a marker for the evaluation.

通过十二烷基硫酸钠 - 丙烯酸酰胺凝胶凝胶电泳免疫印迹，评估了临床梅毒患者的血清中Ig类和IgG抗肿瘤抗体的IgG亚类的反应性。治疗后注意到IgM抗体的反应性明显降低，IgM抗体被证明是评估治疗结果的标志物。但是，在偶尔的情况下，IgM反应性保持低于评估的敏感性下限或未能下降目的地治疗。在有限的原发性和继发型病例中，治疗后IgA抗体减少。在大多数交响乐患者中，在治疗后未观察到IgG抗体的显着降低。在IgG亚类抗体水平上进行的评估表明，治疗后一种或多种IgG亚类抗体的反应性下降。 IgG亚类和类型的pl抗抗原抗原（S）与它们反应，并在患者中反应，但在患者中却有所不同。但是，事实证明，56KD抗原是评估IgM反应性的最可靠的标记抗原。这些结果表明，在IGM抗体无法作为评估标记的情况下，在评估监测IgA和IgG亚类抗体的治疗作用方面非常有用。

项目成果

S.Tanaka and T.Suzuki: "AntiーTreponema pallidum lgM,lgA,and lgG subclass antibody responses after treatment in patients with syphilis at various stages.1.Assesment by enzymeーlinked immunosorbent assay." Genitourinary Medicine. 66. 171-177 (1990)

S.Tanaka 和 T.Suzuki：“不同阶段梅毒患者治疗后的抗梅毒螺旋体 lgM、lgA 和 lgG 亚类抗体反应。1. 通过酶联免疫吸附测定进行评估。” 66. 171-。 177 (1990)

T.Suzuki and S.Tanaka: "AntiーTreponema pallidum IgM,IgA,and IgG subclass antibody responses after treatment in patients with syphilis at various stages.2.Assessments by Westernーblot analysis." Sex.Transmitt.Dis.

T.Suzuki 和 S.Tanaka：“不同阶段梅毒患者治疗后的抗梅毒螺旋体 IgM、IgA 和 IgG 亚类抗体反应。2. 通过蛋白质印迹分析进行评估。”

T.Suzuki and S.Tanaka: "AntiーTreponema pallidum lgM,lga and lgg subclass antibody responses after treatment in patients with syphilis at various stages.2.Assessments by Westernーblot analysis." Sex.Transmitt.Dis.

T.Suzuki 和 S.Tanaka：“不同阶段梅毒患者治疗后的抗梅毒螺旋体 lgM、lga 和 lgg 亚类抗体反应。2.通过蛋白质印迹分析进行评估。”

S. Tanaka and T. Suzuki: "AntiーTreponema pallidum IgM, IgA, and IgG subclass antibody responses after treatment in patients with syphilis at various stages. 1. Assessments by enzymーlinked immunosorbent assay." Genitourin. Med. 66. 171-177 (1990)

S. Tanaka 和 T. Suzuki：“不同阶段梅毒患者治疗后的抗梅毒螺旋体 IgM、IgA 和 IgG 亚类抗体反应。1. 通过酶联免疫吸附测定进行评估。66。171 Med。” -177 (1990)

T. Suzuki and S. Tanaka: "AntiーTreponema pallidum IgM, IgA, and IgG subclass antibody responses after treatment in patients with syphilis at various stages. 2. Assessments by Westernーblot analysis." Sex. Transmit. Dis.

T. Suzuki 和 S. Tanaka：“不同阶段梅毒患者治疗后的抗梅毒螺旋体 IgM、IgA 和 IgG 亚类抗体反应。2. 通过蛋白质印迹分析进行评估。”