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Antigenic Analysis of Treponema Pallidum : Utilization of the TP Antigen(s) in Disease Staging as well as in Evaluating the Result of Treatment.

梅毒螺旋体的抗原分析：利用 TP 抗原进行疾病分期以及评估治疗结果。

基本信息

批准号：
01570581
负责人：
TANAKA Shinobu
金额：
$ 1.34万
依托单位：
Tokyo Metropolitan Institute of Medical Science.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570581/
关键词：
Syphilis Westernーblotting IgG subclass

项目摘要

Changes in reactivities of Ig class and IgG subclass of anti-Treponema pallidum antibodies after treatment in sera from patients with clinical syphilis were assessed by sodium dodecyl sulfateーpolyacrylamide gel electrophoresis immunoblotting. A marked decrease in reactivity of IgM antibodies was noted after treatment and IgM antibody proved to be effective as a marker for evaluating the result of treatment. In occasional cases, however, IgM reactivity remained below the lower limit of sensitivity of the assay or failed to decline despite treatment. IgA antibodies decreased after treatment in limited cases of primary and secondary syphilis. No significant fall of IgG antibody was observed after treatment in the majority of syphilitic patients. Assessment made at the levels of IgG subclass antibodies demonstrated a decline in reactivity of one or more IgG subclass antibodies after treatment. The IgG subclasses and types of T. pallidum antigen(s) reacting with them that responded in conjunction with treatment, nevertheless, varied among patients. However, 56Kd antigen proved to be the most reliable marker antigen for the assessment of IgM reactivity. These results indicated that it is useful in evaluating the effect of treatment to monitor IgA and IgG subclass antibodies in those cases where IgM antibody fails to serve as a marker for the evaluation.

应用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳免疫印迹法检测临床梅毒患者血清中抗梅毒螺旋体抗体IG类和IgG亚类反应性的变化。治疗后IgM抗体反应性明显下降，IgM抗体被证明是有效的评价治疗结果的标志物。然而，在偶尔的情况下，IgM反应性仍然低于测定的灵敏度下限或尽管治疗仍未能下降。治疗后伊加抗体下降，在有限的情况下，一期和二期梅毒。多数患者治疗后IgG抗体无明显下降。在IgG亚类抗体水平上进行的评估表明治疗后一种或多种IgG亚类抗体的反应性下降。T.然而，与它们反应的苍白球抗原在患者中是不同的。然而，56 Kd抗原被证明是用于评估IgM反应性的最可靠的标记抗原。这些结果表明，在IgM抗体不能作为评价指标的情况下，监测伊加和IgG亚类抗体对评价治疗效果是有用的。