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Effects of culture temperatures on radio- and thermosensitivity of human cancer cells.

培养温度对人类癌细胞的放射和热敏感性的影响。

基本信息

批准号：
62570470
负责人：
NAKATSUGAWA Shigekazu
金额：
$ 1.47万
依托单位：
福井医科大学
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570470/
关键词：
culture temperatures human lung cancer xenograft nude mouse Feeder Layer spontaneous multiple metastasis hematogeous metastasis lymphatogenic metastasis hyperthermia culture temperatures human lung cancer xenograft nude mouse Feeder

项目摘要

So far, most of the characteristics of cancer cells have been investigated through culturing at 37.0ﾟC. However, 37.0ﾟC is not always optimal temperature for the growth of cells or repairing damage induced by radiation or hyperthermia. In the treatment for cancer by radiation and other modalities, on the other hand, local control is one of the main purpose, in relevance to cell death. Another purpose pf the therapy may be the prevention and treatment of metastasis. So far, very few metastasis. models derived from human malignancies have been available. As for human lung cancer xenograft, both hematogenous and lymphatogenic route of metastasis has been observed in only one report. In this study, various radio- biological parameters of AOI cells, a cell line derived from human lung cancer, were examined at various culture temperatures, that is 35.0, 36.0, 37.0, 38.0 39.0ﾟC. Culture temperatures showed apparent influences on growth rate, cell density in the plateau phase, thermosensitivit … More ies and Elkind-Sutton type recovery in AOI cells. At 37.0ﾟC, X-ray survival curve in a semilogarithmic graph increased its slope as the doses increased. Moreover, feeder layer AOI cells (1.0- 20x 10^4/6cm petri dish) which were irradiated with 25Gy of X-rays showed a potent cytotoxicities on intact AOI cells at 37.0ﾟC. At 35.0ﾟC, the cytotoxicity of feeder layer was ten times as much as at 37.0ﾟC, while at 39.0ﾟC it was one tenth as much as at 37.0ﾟC. Conditioned media from the feeder layer did show a potent cytotoxic effects on intact AOI cells, irrespective of culture temperatures. So far, feeder layer has been regarded as a growth stimulant. In AOI cells, some toxic substances may be excreted from heavily X-irradiated cells. This cytotoxicity may not be attributed to chromosome aberrations induced by X-rays.When AOI tumors intradermally inoculated into hind legs of KSN nude mice reached 2.5cm in diameter, spontaneous multiple metastases were observed in 100% of the mice. The site of metastases included spleen, lung, kidney, liver and adrenal gland other than lymphnodes of various areas. Each metastatic foci were investigated both macroscopically and microscopically. ^<31>P spectra of AOI tumors were also investigated with MRS (BEM, 2T) after local X-irradiation and or hyperthermia. This system may be very useful for the study on both local control and metastasis of cancer. Less

到目前为止，癌细胞的大多数特征都是通过在37.0 ℃下培养来研究的。然而，37.0 ° C并不总是细胞生长或修复辐射或高温引起的损伤的最佳温度。另一方面，在通过辐射和其他方式治疗癌症时，局部控制是与细胞死亡相关的主要目的之一。治疗的另一个目的可以是预防和治疗转移。到目前为止，很少有转移。已经有了来源于人类恶性肿瘤的模型。至于人肺癌异种移植物，仅在一份报告中观察到血源性和淋巴源性转移途径。本研究测定了人肺癌AOI细胞在35.0、36.0、37.0、38.0、39.0 ℃不同培养温度下的各种放射生物学参数。培养温度对细胞生长速率、平台期细胞密度、温度敏感性、细胞生长速率、细胞生长 ...更多信息 AOI细胞中的ies和Elkind-Sutton型恢复。在37.0 ℃时，X射线存活曲线呈半对数曲线，其斜率随剂量的增加而增大。此外，饲养层AOI细胞（1.0- 20 × 10^4/6cm培养皿）经25 Gy X射线照射后，在37.0 ℃下对完整AOI细胞显示出强烈的细胞毒性。35.0 ℃时饲养层细胞毒性为37.0 ℃时的10倍，39.0 ℃时为37.0 ℃时的1/10。无论培养温度如何，来自饲养层的条件培养基确实对完整AOI细胞显示出强的细胞毒性作用。饲养层一直被认为是一种生长刺激剂。在AOI细胞中，一些毒性物质可从重度X射线照射的细胞中排出。将AOI瘤接种于KSN裸小鼠后肢，瘤体直径达2.5cm时，100%的小鼠出现自发性多发性转移。转移部位除各部位淋巴结外，还包括脾、肺、肾、肝和肾上腺。对每个转移灶进行了肉眼和显微镜检查。用<31>MRS（BEM，2 T）研究了AOI肿瘤局部X线照射和/或热疗后的13 P谱。该系统对肿瘤的局部控制和转移的研究具有重要意义。少