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Early changes and pathogenesis of primitive senile plaque

原始老年斑的早期变化及发病机制

基本信息

批准号：
62570484
负责人：
TAKEDA Shigeki
金额：
$ 1.09万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570484/
关键词：
Senile plaque Primitive senile plaque Amyloid Periodic acid-methenamine silver stain Senile dementia of Alzheimer type 老年期痴呆電顕

项目摘要

1) We have studied the early change of the primitive senile plaque using the serial ultrathin sections stained by periodic acid-methenamine silver (PAM) method. the epoxy-embedded thin section for light-microscopy revealed more delicate details of the amyloid component in the senile plaque. The next ultrathin section for electron-microscopy showed positive silver gains on the amyloid filaments and amyloid-like substance among the neuronal and glial processes. On the other hand, it was difficult to identify primitive senile plaque-like structure only by conventional electron-microscopy without pam stained electron-microscopy. Primitive senile plaque-like structure was composed of neurites, glial processes and pam positive amyloid-like filaments. The last was almost 10nm in diameter and ran sparsely and irregularly between neurites. We consider that they are important ans suggestive findings for the pathogenesis of the primitive senile plaque.2) We have studied the significance of senile plaque in the autopsied brain using by pam stain. One of many examined cases have characteristic histopathological pictures; (1) Numerous senile plaques and moderate neuronal cell loss in the cerebral cortex, (2) a few neurofibrillary tangles in the hippocampus, and (3) well preserved neuron in the subcortical gray matter.Recently, terry et al. proposed a new disease concept, "SDAT without neocortical NFT_s". The histopathology of the cerebral cortex in our patient was very similar to those observed in their patients. However, the above authors did not mention any subcortical changes. Tentatively, we classified the present case as denile dementia with numerous neocortical senile plaques and preserved subcortical nuclei. Although we feel that further clinicopathological studies are necessary, the present case appears to be somewhat different from the well known picture of sdat from a neuropathological viewpoint.

1)本文用过碘酸-六次甲基四胺银（PAM）法连续切片，研究老年斑的早期变化。用于光学显微镜的环氧树脂包埋的薄切片揭示了老年斑中淀粉样成分的更精细的细节。下一张电镜切片显示神经元和神经胶质突起中的淀粉样纤维和淀粉样物质上有阳性银增加。另一方面，不经pam染色，仅用常规电镜难以识别原始老年斑样结构。原始老年斑样结构由神经突起、胶质突起和pam阳性淀粉样纤维组成。第三种直径约10 nm，在突起之间稀疏不规则地分布。我们认为这是原始老年斑发病机制的重要提示性发现。2）我们用pam染色研究了老年斑在尸检脑组织中的意义。其中1例具有特征性的病理组织学表现：（1）大脑皮质内大量老年斑和中度神经元细胞丢失，（2）海马内少量神经元缠结，（3）皮质下灰质神经元保存完好。我们患者的大脑皮层组织病理学与他们的患者非常相似。然而，上述作者未提及任何皮质下变化。因此，我们将本病例归类为老年痴呆症，伴有大量的新皮质老年斑和保留的皮质下核。虽然我们认为进一步的临床病理学研究是必要的，目前的情况下似乎有点不同，从神经病理学的观点众所周知的图片sdat。