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Early changes and pathogenesis of primitive senile plaque

原始老年斑的早期变化及发病机制

基本信息

批准号：
62570484
负责人：
TAKEDA Shigeki
金额：
$ 1.09万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570484/
关键词：
Senile plaque Primitive senile plaque Amyloid Periodic acid-methenamine silver stain Senile dementia of Alzheimer type 老年期痴呆電顕

项目摘要

1) We have studied the early change of the primitive senile plaque using the serial ultrathin sections stained by periodic acid-methenamine silver (PAM) method. the epoxy-embedded thin section for light-microscopy revealed more delicate details of the amyloid component in the senile plaque. The next ultrathin section for electron-microscopy showed positive silver gains on the amyloid filaments and amyloid-like substance among the neuronal and glial processes. On the other hand, it was difficult to identify primitive senile plaque-like structure only by conventional electron-microscopy without pam stained electron-microscopy. Primitive senile plaque-like structure was composed of neurites, glial processes and pam positive amyloid-like filaments. The last was almost 10nm in diameter and ran sparsely and irregularly between neurites. We consider that they are important ans suggestive findings for the pathogenesis of the primitive senile plaque.2) We have studied the significance of senile plaque in the autopsied brain using by pam stain. One of many examined cases have characteristic histopathological pictures; (1) Numerous senile plaques and moderate neuronal cell loss in the cerebral cortex, (2) a few neurofibrillary tangles in the hippocampus, and (3) well preserved neuron in the subcortical gray matter.Recently, terry et al. proposed a new disease concept, "SDAT without neocortical NFT_s". The histopathology of the cerebral cortex in our patient was very similar to those observed in their patients. However, the above authors did not mention any subcortical changes. Tentatively, we classified the present case as denile dementia with numerous neocortical senile plaques and preserved subcortical nuclei. Although we feel that further clinicopathological studies are necessary, the present case appears to be somewhat different from the well known picture of sdat from a neuropathological viewpoint.

1）我们利用高碘酸-六胺银（PAM）法染色的连续超薄切片研究了原始老年斑的早期变化。用于光学显微镜的环氧树脂嵌入薄片显示了老年斑中淀粉样蛋白成分的更微妙的细节。下一个电子显微镜超薄切片显示，神经元和神经胶质过程中淀粉样蛋白丝和淀粉样蛋白样物质上的银含量呈阳性。另一方面，如果没有pam染色电镜，仅通过常规电镜很难识别原始老年斑样结构。原始老年斑样结构由神经突、神经胶质突起和pam阳性淀粉样蛋白丝组成。最后一个直径接近10纳米，在神经突之间稀疏且不规则地延伸。我们认为它们对于原始老年斑的发病机制具有重要意义和提示性发现。2）我们通过pam染色研究了尸检大脑中老年斑的意义。许多检查的病例之一具有特征性的组织病理学图片； (1) 大脑皮质中存在大量老年斑和中度神经元细胞丢失，(2) 海马中存在一些神经原纤维缠结，(3) 皮质下灰质中神经元保存完好。提出了一个新的疾病概念“SDAT without neocortical NFT_s”。我们患者的大脑皮层组织病理学与在他们的患者中观察到的非常相似。然而，上述作者并没有提到任何皮层下的变化。我们暂时将本病例归类为丹尼尔性痴呆，伴有大量新皮质老年斑和保留的皮质下核。尽管我们认为进一步的临床病理学研究是必要的，但从神经病理学的角度来看，本病例似乎与众所周知的 sdat 图像有些不同。