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Study of the expression of oncogene in human thyroid cells

人甲状腺细胞癌基因表达的研究

基本信息

批准号：
62570517
负责人：
IZUMI Motomori
金额：
$ 1.22万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570517/
关键词：
human thyroid cell c-myc c-erbA thyroglobulin thyroid peroxidase e-myc c-EebA 甲状腺ペルオキシダーゼガン遺伝子 c-myc c-erb A

项目摘要

Since oncogenes were discovered in carcinoma virus, these oncogenes have been found to exist in not only carcinoma cells but also normal cells in which they seem to be involved in the proliferation and development of cells. Although many studies of oncogenes have been done in rat and pig thyroid cells, there has been no report on oncogenes in human thyroid cells. These studies were, therefore, undertaken to investigate oncogenes in human thyroid cells.Thyroid cells obtained from Graves' thyroid and normal thyroid at neck surgery were cultured and stimulated by TSH or interleukin 1. Total mRNAs were extracted and Northern blot analysis was performed using ^<32>P-v-myc cDNA. The results revealed that c-myc mRNA transcripts were observed and increased by TSH or interleukin 1 in Graves' thyroid cells while c-myc mRNA were not detected in normal thyroid cells, suggesting that Graves' thyroid cells may express cmyc oncogene in response to stimulation of thyroid stimulating antibodies.Rats we … More re fed low iodine diet and and thyroids were removed and total mRNA was extracted. Northern blot analysis was performed similarly, the results revealed that thyroids obtained from rat fed low iodine diet were enlarged and c-myc mRNA transcript clearly increased compared with control, suggesting that c-myc oncogene may involved in the enlargement of thyroid and that endemic goiter in the area back of iodine may be due to this oncogene expression.ErbA, one of oncogene, was found to be T_3 nuclear receptor gene. Peripheral blood mononuclear cells were studied for erbA mRNA in hyperthyroid patients, hypothyroid patients and normal subjects. The results revealed that erbA mRNA transcript clearly increased in hypothyroidism compared with that of normal and hyperthyroidism, suggesting that T_3 nuclear receptors may increase compensating for deficiency of circulating thyroid hormones.Cytokines have been considered to be involved in autoimmune reaction. Interferon gamma, interferon 1 and 6 were found to inhibit thyroid peroxidase (TPO) and thyroglobulin (Tg) gene expression in human thyroid cell in culture system, suggesting that possible cytokines in autoimmune thyroids may expert negative action on thyroid fundion. Less

自从癌基因在肿瘤病毒中被发现以来，这些癌基因不仅存在于癌细胞中，而且在正常细胞中也存在，它们似乎参与了细胞的增殖和发育。虽然在大鼠和猪的甲状腺细胞中已经进行了许多癌基因的研究，但在人类甲状腺细胞中的癌基因还没有报道。从Graves‘s甲状腺和颈部手术的正常甲状腺中获得甲状腺细胞，用TSH或IL-1刺激甲状腺细胞，提取总mRNAs，用~(32)&lt；32&gt；P-v-myc基因进行Northern印迹分析。结果表明，促甲状腺激素或白介素1可诱导甲状腺细胞表达c-myc基因，而在正常甲状腺细胞中未检测到c-myc基因的表达，提示甲状腺细胞可能表达原癌基因cmyc作为对甲状腺刺激性抗体刺激的反应。…再饲喂低碘饲料，摘除甲状腺，提取总mRNA。Northern印迹分析结果显示，与对照组相比，低碘组大鼠甲状腺体积增大，c-myc基因转录明显增加，提示c-myc癌基因可能参与了甲状腺肿大，碘背侧地方性甲状腺肿可能与该癌基因的表达有关。对甲亢患者、甲减患者和正常人外周血单个核细胞中ERBA基因的表达进行了研究。结果表明，甲状腺功能减退组Erba基因转录水平明显高于正常组和甲亢组，提示T_3核受体可能增强对循环甲状腺激素缺乏的补偿作用，细胞因子可能参与自身免疫反应。干扰素γ、干扰素1和干扰素6可抑制人甲状腺细胞培养体系中甲状腺过氧化物酶(TPO)和甲状腺球蛋白(TG)基因的表达，提示自身免疫性甲状腺中可能存在的细胞因子对甲状腺功能具有负性作用。较少