Hepatocyte Growth Inhibitory Factor (HGI) Derived from HTLV-I (+) T cell Lines ; Effect on the Epidermal Growth Factor-dependent Proliferation of Rat Hepatocyte

源自 HTLV-I ( ) T 细胞系的肝细胞生长抑制因子 (HGI)；

• 批准号: 62570565
• 负责人: INAMOTO Takashi
• 金额: $ 1.34万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570565/
• 关键词: Adult T cell leukemia; Hepatocyte proliferation; Growth in hibitory factor; 上皮増殖因子(EGF)

In the conditioned medium (CM) of an HTLV-I(+) T cell line, ATL-2, we have detected a cytokine that dose-dependently inhibits the epidermal growth factor (EGF)-dependent proliferation of primary cultured rat hepatocytes. Both the increase of cell number and @[^3H]-thymidine incorporation of the hepatocytes were similary inhibited by the addition of this CM. This hepatocyte growth inhibitory factor (HGI) was fractionated at the molecular size of 15,000 to 40,000 using a Sephacryl S-200 column. While the activity of an interleukin-2 (IL-2) receptor inducing factor derived from the same CM, ADF, was overlapping with HGI, further fractionation with ion-exchange column indicated that HGI was different from ADF. However, other HTLV-I transformed T cell lines also co-produced HGI and ADF, suggesting a positive correlation between HGI and ADF. Lymphokines such as IL-2, interleukin-1 (IL-1 ) and interferon- (IFN- ) did not affect hepatocyte proliferation. Because transforming growth factor- (TGF- ) showed a significant inhibitory activity to hepatocyte proliferation, we discuss the relationship between HGI and TGF- . The results suggested that HGI may be involved in the pathophysiology of liver dysfunction of ATL patients and/or in the ragulation of liver rageneration.

在HTLV-I（+）T细胞系ATL-2的条件培养基（CM）中，我们检测到一种细胞因子，其剂量依赖性地抑制原代培养的大鼠肝细胞的表皮生长因子（EGF）依赖性增殖。加入CM后，细胞数的增加和肝细胞的[^3H]-胸苷掺入均受到抑制。使用Sephacryl S-200柱将该肝细胞生长抑制因子（HGI）分级为15，000至40，000的分子大小。虽然来自同一CM的白细胞介素-2（IL-2）受体诱导因子ADF的活性与HGI重叠，但用离子交换柱进一步分级表明HGI与ADF不同。然而，其他HTLV-I转化的T细胞系也共同产生HGI和ADF，表明HGI和ADF之间呈正相关。IL-2、IL-1、IFN-γ等细胞因子对肝细胞增殖无明显影响。由于转化生长因子-（TGF-β）对肝细胞增殖有明显的抑制作用，我们讨论了HGI与TGF-β的关系。提示HGI可能参与ATL患者肝功能损害的病理生理过程和/或参与肝再生的调控。