Induction of Activated Macrophages by Intraperitoneal Injection of MMC-CH, Mitomycin C Adsorbed on Activated Carbon Particles

腹腔注射MMC-CH、活性炭颗粒吸附丝裂霉素C诱导活化巨噬细胞

• 批准号: 62570575
• 负责人: SAKITA Masakazu
• 金额: $ 1.34万
• 依托单位: Meiji College of Oriental Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570575/
• 关键词: peritonitis carcimatosa; Mitomycin C adsorbed on activated carbon particles; activate macrophage; 腹腔マクロファージの活性化; 殺細胞活性; マイトマイシンC; OK-432; 癌性腹膜炎治療; 活性炭吸着マイトマイシンの作用機序; 宿主免疫能への影響

In this study, we examined the effect on the immunological status of hosts and on the cytolytic activity induced in the peritoneal macrophage(PM) by intraperitoneal(IP) injection of MMC-CH, Mitomycin C adsorbed on activated carbon particles and compared it with that of the MMC solution(MMC-AQ) itself. The weight of spleen and thymus of the mice given MMC-CH was decreased lesser than those of the mice given MMC-AQ. Reactivity of spleen cells by Con A was inhibited in the spleen cells from the mice given MMC-AQ more than that from the mice given MMC-CH. Fifty per cent lethal dose was 8 mg/kg in the mice given MMC-AQ, and 16 mg/kg in the mice given MMC-CH. Single IP injection of either MMC-AQ or MMC-CH induced tumoricidal macrophage in the peritoneal cavity. The tumoricidal activity was dependent on the dose of MMC injected and on the effector-to-target ratio and was suppressed by the presence of carrageenan. A significant difference was found in the chronological change of pec between MMC-AQ and MMC-CH administration. One day after MMC-AQ IP injection, the number of PEC wasdecreased markedly, however, it was increased after MMC-CH injection and the number was ten times more than that of MMC-AQ injection. A marked difference in the chronological change of the cytolytic activity of the PM was also observed. In the case of MMC-CH injection, cytolytic activity of PM remained high longer time than that of MMC-AQ. MMC was detected in the soluble fraction of ultrasonicated PM from either MMC-AQ or MMC-CH injected mice and these soluble fraction had a tumoricidal effect on the target cells. Based on these results, it was concluded that there is some participation of intra-cellular drug within the MMC-induced PM in tumoriciadal effects, especially after MMC-CH injection.

本研究观察了腹腔注射活性炭吸附丝裂霉素C（MMC-CH）和丝裂霉素C（MMC-AQ）对宿主免疫状态和腹腔巨噬细胞（PM）杀伤活性的影响，并与MMC溶液（MMC-AQ）进行了比较。MMC-CH组小鼠脾脏和胸腺重量的下降幅度小于MMC-AQ组。MMC-AQ组小鼠脾细胞对Con A反应性的抑制作用大于MMC-CH组，MMC-AQ组小鼠的半数致死量为8 mg/kg，MMC-CH组小鼠的半数致死量为16 mg/kg。杀肿瘤活性依赖于MMC注射的剂量和效应物与靶标的比率，并且通过角叉菜胶的存在而被抑制。MMC-AQ和MMC-CH给药组pec的时间变化有显著性差异。腹腔注射MMC-AQ后1d，PEC数量明显减少，而注射MMC-CH后PEC数量明显增加，是注射MMC-AQ后的10倍。还观察到PM的细胞溶解活性的时间变化的显著差异。在注射MMC-CH的情况下，PM的溶细胞活性比MMC-AQ保持高的时间更长。在MMC-AQ或MMC-CH注射小鼠的超声处理PM的可溶性部分中检测到MMC，并且这些可溶性部分对靶细胞具有杀肿瘤作用。基于这些结果，可以得出结论，在MMC诱导的PM中，细胞内药物在一定程度上参与了杀肿瘤作用，特别是在MMC-CH注射后。

项目成果

岩本昭彦,〓田雅一,大田幸樹,高橋俊雄: 癌と化学療法.

Akihiko Iwamoto、Masakazu Ota、Koki Ota、Toshio Takahashi：癌症与化疗。

岩本昭彦、咲田雅一、高橋俊雄 他: 日本外科学会雑誌. 90(11). (1989)

Akihiko Iwamoto、Masakazu Sakuta、Toshio Takahashi 等：日本外科学会杂志 90(11) (1989)。

A. Iwamoto; M. Sakita; T. Takahashi et al.: "Effect of Intraperitoneal Administration of MMC-CH, Mitomycin C Adsorbed on Activated Carbon Particles on Immunological status of hosts." J. Jpn. Surg. Soc.90(11). (1989)

A.岩本；

咲田雅一、岩本昭彦、高橋俊雄 他: 日本外科学会雑誌.

Masakazu Sakuta、Akihiko Iwamoto、Toshio Takahashi 等人：日本外科学会杂志。

岩本昭彦、咲田雅一、高橋俊雄 他: 日本外科学会雑誌. 90 (11). (1989)

Akihiko Iwamoto、Masakazu Sakuta、Toshio Takahashi 等：日本外科学会杂志 90 (11) (1989)。