Nuclear DNA analysis of genetics of cancer and dysplasia complicating ulcerative colitis

癌症和溃疡性结肠炎并发不典型增生的遗传学核 DNA 分析

• 批准号: 62570593
• 负责人: NAGAI Hideo
• 金额: $ 0.9万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570593/
• 关键词: Microspectrophotometry; DNA; Ulcerative colitis; Dysplasia; Aneuploid; Polyploid; Surueillance colonoscopy; 細胞核DNA量; 癌化

1)Assessment of optimum thickness of the sections : This study assessed the optimum thickness of the paraffin embedded sections to determine the true DNA content with a cytophofometric technique in 35 colonic samples from 23 patients with ulcerative colitis and 10 non-colitic patients with colorectal cancer. The 4 ,7 ,10 sections were cut from each samples. The cellular DNA values of these sections were compared with smears from cells detached from the same paraffin embedded tissue. The modal DNA values of the four methods were similar. On the contrary, the frequency of the cells with DNA content higher than 6C value (polyploid cells) did not coincide. The frequency of false negative cases which had polyploid cells in smears but hadn't in sections was 8.6, 0, 5.7 in 4, 7, 10 respective. The frequency of false positive cases was 14.3, 20, 25.7, but 6.3, 6.7, 18.8 in the inactive specimens. Therefore 7 section showed higher sensitivity and specificity. We suqqest that micro spectrophotom … More etric DNA content in ulcerative colitis is better analized with 7 section.2)Application for cancer and dysplasia diagnpsis : The DNA content was measured by microspectrophotometry from 100 specimens in 60 patients with ulcerative colitis including six complicating carcinoma. In dysplastic 23 (77%) of 30 samples showed aneuploidy or polyploidy, whereas in non-dysplastic tissues 50 (94%) of 53 samples were diploid, the difference being statistically significant. Polyploidy was often observed in the non-dysplastic mucosa of the patients complicating cancer or dysplasia. In non-dysplastic patients all sample with inflammation showed diploidy, however 10% of samples without inflammation showed polyploidy. A good correlation was found between the incidence of polyploid cells and the grade of dyspasia. From this study it was assumed that microspectrophotometric measurement of DNA content is useful in the assessment and siagnosis of dysplasia in ulcerative colitis and can be available in the screening of high-risk patients. Less

1)评估最佳厚度的切片：本研究评估了石蜡包埋切片的最佳厚度，以确定真正的DNA含量与cytophofometric技术在35个结肠样本从23例溃疡性结肠炎患者和10例非结肠炎患者结直肠癌。从每个样品上切下4、7、10个切片。将这些切片的细胞DNA值与来自从相同石蜡包埋组织分离的细胞的涂片进行比较。四种方法的DNA众数相近。而DNA含量高于6C值的细胞（多倍体细胞）出现的频率则不一致。涂片中有多倍体细胞而切片中无多倍体细胞的假阴性率分别为8.6/4、0/7、5.7/10。假阳性率分别为14.3、20、25.7，而非活性标本假阳性率分别为6.3、6.7、18.8。因此7个切片具有较高的敏感性和特异性。我们建议显微分光照相机 ...更多信息 用显微分光光度法测定了60例溃疡性结肠炎（包括6例并发癌）100例标本的DNA含量。在异型增生中，30个样本中有23个（77%）表现为非整倍体或多倍体，而在非异型增生组织中，53个样本中有50个（94%）为二倍体，差异具有统计学意义。在非异型增生的癌或异型增生患者的粘膜中常观察到多倍体。在非发育异常患者中，所有具有炎症的样品均显示二倍体，然而10%的无炎症的样品显示多倍体。多倍体细胞的发生率和消化不良的程度之间有很好的相关性。从这项研究中，它被认为是DNA含量的显微分光光度测量是有用的评估和诊断的溃疡性结肠炎的异型增生，并可以在筛选高危患者。少

项目成果

正木忠彦 他: 胃と腸. 24. (1989)

Tadahiko Masaki 等人：胃和肠 24。（1989）

Tadahiko M.; et al:"A microspectrophotometric study of colorectal adenomas" Stomach and Intestine. 24. 1989

忠彦 M.；

小西文雄: Medicina. 24. 208-209 (1987)

小西文雄：医学。24。208-209（1987）

Kimitaka S.; et al:"Microspectrophotanetric DNA analysis in ulcerative colitis with special referrence to its application for cancer and dysplasia diaganosis." Japanese Journal of Gastroenterology.

基米塔卡·S.；

Tadahiko M.; et al:"A microspectrophotometric study of the nuclear DNA content of coloretal adnoma with reference to its grade of atypia" Journal of Japan Surgical Society. 89. 1315 (1988)

忠彦 M.；