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A study on intracellular energy status of the preserved liver by 31P-NMR spectroscopy

31P-NMR光谱研究保存肝脏细胞内能量状态

基本信息

批准号：
62570580
负责人：
TERAOKA Satoshi
金额：
$ 0.96万
依托单位：
The third department of surgery,Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570580/
关键词：
Liver transplantation 31P-NMR spectrometer 31P-NMR spectroscopy 肝保存温阻血

项目摘要

Intracellular high energy phosphate of the preserved mouse liver was measured by 31P-MMR spectrometer The measurements were done either under warm or cold ischemic condition. After ischimea, the liver was reperfused with modified Krebs-Henseleit buffer solution and reevaluted.ATP decreased rapidly and became undetectable within 10 min. under both warm and cold ischemia.Synthesis of ATP was observed again when reperfusion was started on these ischemically preserved liver. ATP was resynthesised in the liver preserved shorter than 30 min. in case of warm ischemia and 10 hr. in case of cold ischemia. Infusion of the Ca channel blocker(verapamil) in to the portal vein prior to hepatectomy was protective against a decrease in the synthesis of ATP at the time of reperfusion. The ratio of MP/Pi was maintained at high level in the liver which showed good recovery of ATP at the time of reperfusion.OUR results demonstrated the fact ATP has limited value on judgement of the viability of the preserved organ particulary under non-perfused state. While the MP/Pi ratio may be useful as an indicator available to the same condition.

用~（31）P-MMR谱仪测定了保存小鼠肝细胞内高能磷酸盐的含量。缺血后，用改良Krebs-Henseleit缓冲液再灌注，再测ATP含量，发现热缺血和冷缺血10 min内ATP含量均迅速下降至检测不到，再灌注时，ATP的合成再次出现。在热缺血情况下保存短于30分钟，在冷缺血情况下保存短于10小时的肝脏中再合成ATP。肝切除术前门静脉注射钙通道阻滞剂（维拉帕米）对再灌注时ATP合成减少具有保护作用。结果表明，在再灌注时，肝脏MP/Pi比值维持在较高水平，ATP的恢复较好，提示ATP在判断保存器官的存活性，特别是在非再灌注状态下，其价值有限。而MP/Pi比率可用作可用于相同条件的指标。