Histological Study of the Granulation Tissue around The Loosened Prosthesis

对全关节置换术翻修手术时获得的18例标本进行组织学研究。在骨水泥界面处采集肉芽组织标本。在肉芽组织中观察到的大多数细胞似乎是巨噬细胞样细胞，细胞质相对丰富。利用抗巨噬细胞单克隆抗体（63D3）进一步研究表明，这些细胞来源于巨噬细胞。在偏振光显微镜下，在一些细胞中发现了大量高密度聚乙烯碎片。电镜观察显示，这些细胞有大量的空泡，胞浆中含有溶酶体，提示这些细胞产生溶酶因子如PGE1和胶原蛋白，对骨水泥界面的假体松动起重要作用。

Eighteen specimens obtained at the time of revision sungery of total joint replacement were studied histologically. Specimens of the granulation tissue were collected from the bone-cement interface. Most of the cells observed in this granulation tissue seemed to be macrophage-like cells which had relatively abundant cytoplasm with H2 E staining. Further study utilized anti-macrophage monoclonal antibody (63D3) showed that these cells were macrophage origin. With polarized light microscopy, abundant fragments of high density polyethylene were found in some of these cells. Finally, electronmicroscopic observation revealed that these cells had numerous vacuoles which included lysosomes in their cytoplasm suggesting that these cells produced orteolytic cytokine such as PGE1 and collagenese and played important role for the loosening of the prosthesis at bone-cement interface.