Histological observations of the muscle tissue reaction to sintered hydroxyapatite.

将烧结型羟基磷灰石块植入10只Wistar大鼠的胫前肌和胫骨骨膜之间，20只跟腱和胫骨之间，40只跟腱内。光镜检查均见羟基磷灰石与肌肉或肌腱交界处有结缔组织形成，并长入羟基磷灰石块的微孔内。结缔组织在51~150微米孔内最大植入深度为350微米。肌骨膜包埋组最大抗拉强度为0.9 kg/cm2，肌腱内包埋组术后8周最大抗拉强度为1.3 kg/cm2。光镜下，肌肉骨膜包埋组羟基磷灰石块与骨膜之间有膜性骨化，而肌腱内包埋组羟基磷灰石块周围无钙化或无新骨形成，因此认为羟基磷灰石是一种具有膜性成骨能力并与肌肉或肌腱机械结合的材料。在未来，羟基磷灰石在骨科手术中的新应用有待于研究。

The sintered porous hydroxyapatite block was embedded between the tibial anterior muscle and the periosteum of tibia in 10 Wister rats, between the calcaneal tendon and the tibia in 20, into the calcaneal tendon in 40. Microscopical examination revealed newly formed connective tissue at the interface between the hydroxyapatite and the muscle or the tendon in all cases and its growth into the micropores of hydroxyapatite block. The maximal ingrowth depth of connective tissue was 350 mum in 51-150 mum pore. In muscle-periosteum embedded group, the maximal mechanical strength against tension was 0.9 kg/cm^2, in intra-tendon embedded group, it was 1.3 kg/cm^2 8 weeks after implantation. Microscopicaly, membranous ossification was noted between the hydroxyapatite block and the periosteum in muscle-periosteum embedded group, but no calcification or no new bone formation around the hydroxyapatite blocks in intra-tendon embedded group.Therefore, hydroxyapatite was considered to be one of the materials which had ability of membranous ossification and mechanically bonded to muscle or tendon. In future, new applications of hydroxyapatite for orthopedic surgery are to be investigated.