Clinical Application of Bone Morphogenic Protein (BMP) using Collagen Biomaterials

胶原生物材料骨形态发生蛋白（BMP）的临床应用

• 批准号: 62570696
• 负责人: FUJII Katsuyuki
• 金额: $ 1.02万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570696/
• 关键词: Bone morphogenic protein (BMP); Collagen biomaterial; 骨誘導; 骨形成因子(BMp); 骨形成因子(BMP); 移植

Recent studies have demonstrated that bone and osteosarcoma tissur contains a highly insoluble protein which induces differentiation of mesenchymal-type cells into cartilage and bone. In order to apply this protein, BMP, in the clinical field, we have investigated bone inductive ability of BMP combined with several types of collagen biomaterials as described below. 1. Collagen biomaterials 1) Collagen sponge 2) Collagen solution 3) Collagen membrane 2. Bmp-collagen complex was transplanted into thigh muscle or bone defect region of mouse, and roentgenic and histological observations were performed. Results Although bone inductive ability of BMP with each type of collagen biomaterial was observed, BMP-2%collagen solution mixture was found to show most obvious bone formation either in muscle tissue or bone defect area. BMP used in the present study were isolated from calf bone and human papova virus induced osteosarcoma in hamster designayed as Os-515. Ectopic bone formation in muscle tissues was detected around 3-4 weeks after implantaion, and collagen sponge and collagen membrane were found to absorved around 6-7 week. Conclusion It was found that collagen biomaterials were suitable carriers to keep BMP at the same area until the differentiation of mesenchymal cells into cartilage and bone. These collagen biomaterials were auto-digestable and shown to have no significant tissue reactions. Thus, there may be a good possibility of clinical use of MBP-collagen biomaterial complex.

最近的研究表明，骨和骨肉瘤组织含有高度不溶性蛋白质，可诱导间充质型细胞分化为软骨和骨。为了将这种蛋白质BMP应用于临床领域，我们研究了BMP与几种类型的胶原生物材料结合的骨诱导能力，如下所述。 1.胶原生物材料 1）胶原海绵 2）胶原溶液 3）胶原膜 2.将Bmp-胶原复合物移植到小鼠大腿肌肉或骨缺损区域，并进行射线和组织学观察。结果虽然观察了BMP与每种类型胶原生物材料的骨诱导能力，但发现BMP-2%胶原蛋白溶液混合物在肌肉组织或骨缺损区域显示出最明显的骨形成。本研究中使用的BMP是从小腿骨和人乳头瘤病毒诱导的仓鼠骨肉瘤中分离出来的，命名为Os-515。植入后3-4周左右发现肌肉组织异位骨形成，6-7周左右发现胶原海绵和胶原膜吸收。结论发现胶原生物材料是使BMP保持在同一区域直至间充质细胞分化为软骨和骨的合适载体。这些胶原蛋白生物材料可自动消化，并且没有明显的组织反应。因此，MBP-胶原蛋白生物材料复合物具有良好的临床应用可能性。

项目成果

Takeuchi,H. Fujii,K.Tajiri,K.Sai,S. Marumo,K.Ohta,M,Ohhasi,T. and Murat,K.: Transactions of the 34th Annual Meeting of Orthopaedic Research Society(U.S.A). VOL.13. 323 (1988)

竹内，H.

Takeuchi,H.;Fujii K.;Tajiri,K.;Sai,S.;Marumo K.;Ohta M.;Ohhasi,T.;Murota,K.: Transaction of the 34th Annual Meeting of Orthopaedic Reserch Society(USA). 13. 323 (1988)

Takeuchi,H.;Fujii K.;Tajiri,K.;Sai,S.;Marumo K.;Ohta M.;Ohhasi,T.;Murota,K.：第 34 届骨科研究学会年会论文集（美国）

Takeuchi H.;Fujii K.;Tajiri K.;Sai S.;Marumo K.;Ohta M.;Ohhasi T.;Murota K.: Transaction of the 34the Annual Meeting of Onthopaedic Research Society (USA). 13. 323 (1988)

Takeuchi H.；Fujii K.；Tajiri K.；Sai S.；Marumo K.；Ohta M.；Ohhasi T.；Murota K.：第 34 届骨科研究学会年会（美国）交易。

藤井克之,太田充宣,辻美智子,大橋俊子,室田景久,宮田暉夫: 骨軟骨移植研究会抄録. 5. 40 (1987)

Katsuyuki Fujii、Mitsunori Ota、Michiko Tsuji、Toshiko Ohashi、Kagehisa Murota、Akio Miyata：骨软骨移植研究组摘要 5. 40 (1987)。

藤井克之、太田光宣、辻美智子、大橋俊子、室田景久、宮田暉夫: 骨軟骨移植研究会抄録. 5. 40 (1987)

Katsuyuki Fujii、Mitsunobu Ota、Michiko Tsuji、Toshiko Ohashi、Kagehisa Murota、Akio Miyata：骨软骨移植研究组摘要 5. 40 (1987)。