Studies on Oncotrophoblast Antigens by Monoclonal Antibodies

为了研究肿瘤滋养层细胞抗原，已制备了8种与正常和/或肿瘤性人滋养层细胞反应的单克隆抗体（MoAb），并检测了它们与其他非滋养层起源肿瘤的反应性。此外，已经研究了几种MoAb对体内肿瘤的亲和力。将6株单克隆抗体（TM 7 -3、TM 3 -8、TM 5 -1、TM 8 -13、TM 4 -5和TM 3 -3）的免疫原性基因转染培养的绒毛膜癌细胞系，根据单克隆抗体所检测的抗原分布，TM 7 -3和TM 3 -8、TM 4 -5和TM 3 -3的反应模式相似。其中，TM 7 -3和TM 3 -8均表现出独特的反应模式。尽管在正常绒毛膜绒毛中的反应非常弱，但它们与绒毛膜癌，特别是细胞滋养层样肿瘤细胞的反应非常强。卵巢浆液性囊腺癌（3/4）和高分化子宫体癌（2/2）呈阳性反应。对<131>绒毛膜癌（BeWo）移植裸鼠注射13 I标记的TM 3 -8后6天进行的闪烁照相术显示肿瘤的良好可视化。肿瘤和其他正常组织的放射性在注射后第6天显示TM 3 -8与肿瘤的选择性结合。在<125>对患绒毛膜癌的小鼠注射13 I标记的TM 3 -8后4天，进行小鼠全身放射自显影。放射自显影显示肿瘤内有明显的阳性反应，目前所研究的单克隆抗体基本上都与滋养层细胞或绒毛膜癌有关。然而，它们分别以不同的模式对某些种类的非滋养层起源的肿瘤反应。这些发现与早期报道的癌细胞表型改变为滋养层细胞一致。此外，对于一些单克隆抗体，获得了令人鼓舞的数据，以考虑实际应用的可能性，例如在临床领域中的肿瘤成像。

In order to investigate oncotrophoblast antigens, 8 monoclonal antibodies (MoAbs) which react with normal and/or neoplastic human trophoblasts have been produced, and examined their reactivities with other non-trophoblast origin tumors. In addition, several MoAbs have been studied for their affinities to the tumor in vivo. Immunogene of the 6 MoAbs (TM7-3,TM3-8,TM5-1, TM8-13,TM4-5 and TM3-3) were cultured choriocarcinoma cell lines.According to the distribution of the antigens detected by the MoAbs, TM7-3 and TM3-8, TM4-5 and TM3-3 showed a similar reactive pattern respectively. Among there MoAbs, both TM7-3 and TM3-8 showed an unique reactive pattern. They demonstrated a very strong reaction with choriocarcinoma, particularly cytotrophoblast-like tumor cells, in spite of a very weak reaction in normal chorionic villi. In addition, they showed a positive reaction with ovarian serous cyst adenocarcinomas(3/4) and well differentiated uterine corpus carcinoma(2/2). Scintigraphy performed 6 days after injection of ^<131>I-labeled TM3-8 to choriocarcinoma(BeWo) transplanted nude mice, demonstrated excellent visualization of the tumor. Radioactivities of the tumor and other normal tissues at 6th days from the injection, showed a selective binding of TM3-8 to the tumor. Whole body autoradiography of mouse was performed 4 days after injection ^<125>I-labelled TM3-8 to the mice with a choriocarcinoma. The autoradiogram showed a clear positive gains restricted to the tumor.All MoAbs in the present studies are related to trophoblast or chorio- carcinoma basically. they react, however, to some kinds of non-trophoblast origin tumors in various patterns respectively. These findings, are consistent with earlier reports of cancer cells phenotypic change to trophoblast. In addition, with some of the MoAbs, encouraging data was obtained for thinking of a possibility of the practical application such as imaging of tumors in clinical field.