An Experimental Study on the Biological Property of Periodontal Pocket-epithelium.

采用免疫组化、电镜及示踪等方法研究牙周袋上皮的生物学特性。在大鼠上颌第一磨牙和第二磨牙之间置入弹性齿龈制成实验袋。抗角蛋白抗体免疫组化标本中，袋上皮呈阳性染色，正常结膜上皮呈阴性染色。袋上皮和结上皮外基底层抗层粘连蛋白抗体均呈阳性反应。电镜观察袋上皮呈多角形，胞质中央有一个大核。细胞器发育不全，内质网和高尔基体较少，张力丝较少。可以发现牙周袋形成的初始变化，即细胞间连接的破坏，而不是牙齿表面上皮细胞的脱落。本研究利用过氧化物酶和镧，检测到示踪剂通过袋上皮细胞间隙进入牙周袋。也就是说，口袋上皮具有允许某些物质通过这些细胞间隙的结构。上皮下方的结缔组织中可见丰富的毛细血管。与此相反，在长连接上皮对应的结缔组织中，很少检测到毛细血管。根据这些结果，我们认为；(1)袋上皮可能是低分化的角化细胞；(2)袋上皮具有允许某些物质通过其细胞间隙的结构，因此，袋上皮像正常的结上皮一样，由宫颈液体提供保护功能；(3)检查牙侧层粘连蛋白的存在可有效地确定是结上皮还是袋上皮。

To elucidate the biological property of periodontal pocket epithelium, immunohistochemistry, electron microscopy and tracer methods were employed. Experimental pocket was produced by insertion of elastic gums between the first and second molar of the rat maxilla. In specimens of immunohistochemistry using anti-keratin antibody, pocket epithelium showed positive staining, while normal junctional epithelium revealed negative. Outer basal lamina of both pocket and junctional epithelia showed positive reaction to anti-laminin antibody. In electron microscopic observation the pocket epithelium was polygonal in shape, and had a large nucleus located in the center of the cytoplasm. Poor-developed organelle, few rough endoplasmic reticulum and Golgi apparatus but a small amount of tonofilaments, was detected. Initial changes of periodontal pocket formation could be found, i.e., as a destruction of cell-to cell connection but not an exfoliation of epithelial cells from the tooth surface. It could be detected that tracers passed through intercellular spaces of the pocket epithelium into periodontal pocket in the present stuey using peroxidase and lanthanum. Namely, the pocket epithelium was provided structures allowing to pass some materiasl through tese intercellular spaces. Abundant capillaries were observed in the connective tissues just below the epithelium. On the contrary, few capillaries could be detected in the connective tissue corresponding to the long junctional epithelium. From these results, it is suggested that ; (1) pocket epithelium may be poorly differentiated keratinizing cell, (2) pocket epithelium has structures to allow passing some materials through their intercellular spaces, consequently, pocket epithelium is provided a protective function by cervical fluids in similar manner of normal junctional epithelium, (3) to examine the presence of laminin at the tooth side is effective to determine junctional epithelium or pocket epithelium.