Analysis and assessment of relationship between the expression levels of ras oncogane product p21 in carcinomas of oral mucosa and their clinical tuma status

1. 我们采用单克隆抗体和免疫组化方法检测了121例鳞状细胞头颈部癌组织切片中ras p21的表达。在121例患者中，59例标本显示ras p21阳性染色。另一方面，44例口腔白斑和58例正常粘膜未见阳性染色。我们分析了43例患者，其中27例已治疗，无肿瘤，随访至少5年，16例死亡，分析了ras p21在原发肿瘤部位的表达与临床转归的关系，以及与临床病理肿瘤状态的关系。ras p21在43例患者中表达显著不良，p<0.001。在121例患者的组织学分级分析中，ras p21在I+II级和III级的表达均显著预后差（p<0.05和p.0.0005）。ras p21表达与预后不良的相关性与临床分期有关，在ⅲ期（p<0.05）和ⅲ+ⅳ期（p<0.005）。检测ras p21在原发肿瘤中的表达与局部淋巴结网络停滞的关系，差异有统计学意义（p<0.06）。此外，43例患者中ras p21的表达与烟草使用有显著相关性。提示ras p21在头颈部鳞状细胞癌组织中的表达与肿瘤的临床病理状态及临床预后相关。应用免疫par氧化酶染色和免疫浮法检测了Ras癌基因产物p21在乳头状癌鳞癌新鲜肿瘤组织中的表达。因此，在预处理时的组织切片中，ras p21的表达呈强阳性染色。然而，与hicpsy材料相比，完成癌症治疗后获得的肿瘤组织中ras p21的含量明显降低。这些发现表明，一些癌症治疗的效果可以通过肿瘤组织中ras p21的表达来估计。人涎腺腺腺癌细胞系HSG高水平表达ras癌基因p21。当HSG细胞在二丁基环AMP （dB-cAMP）存在下培养时，结果表明，随着添加dB-cAMP的浓度不同，HSG细胞的集落形成能力受到抑制。此外，与未处理的HSG细胞相比，处理后的HSG细胞中ras p21的表达降低。此外，我们发现在HSG细胞中dB-CAMP浓度与细胞内cAMP浓度之间有统计学意义。少

1. We examined the expression of ras p21 in tissue sections from 121 patients with squamous cell head and neck cancer using monoclonal antibody and immunohisto chemical nethod. Among the 121 patients examined, 59 specimens showed positive staining ras p21. On the hand, no 44 oral leukoplakies and 58 normal mucosae were seen to be positivaly stained. A total of 43 patients including 27 treated, tumor-free cases, who have been follored for at least 5 years and 16 dead cases, were analyzed for the relationship hetween ras p21 expression in the primary tumor sites and clinical outcome in relation to the clinico pathological tumor status. The expression of ras p21 in 43 patients was significantly poor prognesis at p<0.001. When 121 patients were analyzed in relation to the histological graling, ras p21 expression in grade I+II and grade III was significantly poor prognosis at p<0.05 and p.0.0005, respectively. In addition, correlation hetween ras p21 expression and poor prognosis with regar … More d to the clinical staging was signifiasnt in stage III (p<0.05) and stage III+IV (p<0.005). The examination of relationship hstween ras p21 expression in the primary tumors and regional lyngsh node netostasis resulted in statistical significance (p<0.06). Moreover, expression of ras p21 in the 43 pateints showed significant relation with tohacco use. These findings suggest that ras p21 expression in squamous cell head and neck cancer tissue is conelated with the clinico pathological tumor status and clinical outcome.2. Ras oncogene product p21 expression in fresh tumor tissue from a squamous cell careinoma of the naeillary sincos was examined by the use of immunoparoxidase staining and immunoflotting. As a consequence, expression of ras p21 in tissue sections taken at pretreatment time showed strongly positive stain. However, the content of ras p21 present in the tumor tissues obtained after completion of cancer treatment was markedly reduced, when compared with the hicpsy material. These findiags indicate that effect of some cancer therapy could he estimated from ras p21 expression in tumor tissues.3. A human salivary qland sdenecarinoma cell line, HSG, expresses ras oncogend p21 at a high level. When HSG cells were cultured in the presence of dibutyigl cyclic AMP (dB-cAMP), it was shown that colonyforming ability of HSG cells was suppressed in accordance with the concentration of added dB-CAMP. In additon, ras p21 expression in the treated HSG cells was reduced, when compared with the untreated HSG cells. Moreover, it was found that conelation hetween the concentralions of dB-CAMP and intracellular cAMP in the HSG cells was statistically significant. Less