Analysis and assessment of relationship between the expression levels of ras oncogane product p21 in carcinomas of oral mucosa and their clinical tuma status

1.应用单克隆抗体和免疫组化方法检测了121例头颈鳞癌组织中rasp 21的表达。在121例患者中，59例ras p21阳性。44例口腔粘膜白斑和58例正常口腔粘膜均未见阳性染色。本文分析了43例原发性肝癌患者（其中27例经治疗，无瘤，随访5年以上，16例死亡）原发灶ras p21表达与临床病理结果的关系。43例ras p21阳性表达者预后差，P<0.001。当分析121例患者与组织学分级的关系时，rasp 21表达在I+II级和III级中分别是显著不良预后，p<0.05和p <0.0005。此外，rasp 21表达与预后不良之间的相关性与regar 关于我们 d与临床分期的关系在Ⅲ期（p<0.05）和Ⅲ + Ⅳ期（p<0.005）无显著性差异。原发灶ras p21表达与淋巴结转移的关系有统计学意义（p<0.06）。ras p21蛋白表达与激素使用密切相关。提示rasp 21在头颈部鳞状细胞癌组织中的表达与肿瘤的临床病理状态及临床预后有关.应用免疫过氧化物酶染色和免疫印迹法检测了1例鼻咽癌新鲜组织中Ras癌基因产物p21的表达。结果，在预处理时间取的组织切片中ras p21的表达显示强阳性染色。然而，当与打嗝材料相比时，在完成癌症治疗后获得的肿瘤组织中存在的ras p21的含量显著降低。这些结果提示，rasp 21在肿瘤组织中的表达可以作为某些肿瘤治疗效果的评价指标.人涎腺腺癌细胞系HSG高表达ras癌基因p21。当HSG细胞在存在二丁基环腺苷酸（dB-cAMP）的情况下培养时，显示HSG细胞的集落形成能力根据所添加的dB-cAMP的浓度而受到抑制。另外，与未经处理的HSG细胞相比，经处理的HSG细胞中ras p21表达降低。此外，还发现HSG细胞内的dB-cAMP浓度与细胞内cAMP浓度之间有显著性差异。少

1. We examined the expression of ras p21 in tissue sections from 121 patients with squamous cell head and neck cancer using monoclonal antibody and immunohisto chemical nethod. Among the 121 patients examined, 59 specimens showed positive staining ras p21. On the hand, no 44 oral leukoplakies and 58 normal mucosae were seen to be positivaly stained. A total of 43 patients including 27 treated, tumor-free cases, who have been follored for at least 5 years and 16 dead cases, were analyzed for the relationship hetween ras p21 expression in the primary tumor sites and clinical outcome in relation to the clinico pathological tumor status. The expression of ras p21 in 43 patients was significantly poor prognesis at p<0.001. When 121 patients were analyzed in relation to the histological graling, ras p21 expression in grade I+II and grade III was significantly poor prognosis at p<0.05 and p.0.0005, respectively. In addition, correlation hetween ras p21 expression and poor prognosis with regar … More d to the clinical staging was signifiasnt in stage III (p<0.05) and stage III+IV (p<0.005). The examination of relationship hstween ras p21 expression in the primary tumors and regional lyngsh node netostasis resulted in statistical significance (p<0.06). Moreover, expression of ras p21 in the 43 pateints showed significant relation with tohacco use. These findings suggest that ras p21 expression in squamous cell head and neck cancer tissue is conelated with the clinico pathological tumor status and clinical outcome.2. Ras oncogene product p21 expression in fresh tumor tissue from a squamous cell careinoma of the naeillary sincos was examined by the use of immunoparoxidase staining and immunoflotting. As a consequence, expression of ras p21 in tissue sections taken at pretreatment time showed strongly positive stain. However, the content of ras p21 present in the tumor tissues obtained after completion of cancer treatment was markedly reduced, when compared with the hicpsy material. These findiags indicate that effect of some cancer therapy could he estimated from ras p21 expression in tumor tissues.3. A human salivary qland sdenecarinoma cell line, HSG, expresses ras oncogend p21 at a high level. When HSG cells were cultured in the presence of dibutyigl cyclic AMP (dB-cAMP), it was shown that colonyforming ability of HSG cells was suppressed in accordance with the concentration of added dB-CAMP. In additon, ras p21 expression in the treated HSG cells was reduced, when compared with the untreated HSG cells. Moreover, it was found that conelation hetween the concentralions of dB-CAMP and intracellular cAMP in the HSG cells was statistically significant. Less