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Research for Cell Behavior of Osteoclasts Receiving Mechanical Pressure

机械压力下破骨细胞细胞行为的研究

基本信息

批准号：
62570911
负责人：
KURIHARA Saburo
金额：
$ 1.22万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570911/
关键词：
osteoclast pressure direct mechanical stimulus activation PMA cell fusion of osteoclast 変性型破骨細胞歯科矯正学 16mm顕微鏡映画

项目摘要

The research projects in this grant of cell behavior of osteoclasts during mechanical pressure in vitro were divided into four groups: 1)Pressure into closed circuit of osteoclast culture system, 2)Direct mechanical pressure onto osteoclasts, 3)Behavior of activated osteoclasts for bone resorption and 4)Serial cinematograpic observations on degenarated osteoclast.The results were summarized as follows: 1)The increase of numbers of preosteoclasts as well as multinucleated osteoclasts and the activated bone resorption were observed by means of 16mm cinematography after indirect pressure of about 50mmHg was given into closed circuit of Rose chamber culture system. 2)Direct pressure of culture medium from a jet nozzle of microcapillary to osteoclasts showing bone resorption. These cells showed inhibition of bone resorption just after reception of the direct mechanical pressure. The cells showed the same pattern of the cell behavior after reception of direct mechanical pressure by microcapillary itself. 3)Osteoclastic cell fusion which represents one process of active bone resorption by osteoclasts, were recorded by cinematography. The other process of the activity was demonstrated by using Phorbol Myristate acetate (PMA) onto an osteoclast by direct administration through microcapillary. 4)It was also recorded by cinematography that an osteoclast showed degenerative pattern after long term culture and that it was finally phagocytized by macrophases around the cell.

本课题对体外机械压力下破骨细胞的细胞行为研究分为四组：1)破骨细胞培养系统闭合压力组；2)破骨细胞直接机械压力组；3)活化破骨细胞骨吸收行为组；4)退化破骨细胞连续摄影观察组。结果如下：1)在玫瑰花室培养系统闭路中间接施加约50mmHg压力后，采用16mm摄影观察破骨前细胞和多核破骨细胞数量的增加和骨吸收的活化。2)将培养基从微毛细血管喷嘴直接压向破骨细胞，显示骨吸收。这些细胞在接受直接机械压力后表现出骨吸收抑制。细胞在受到微毛细血管自身直接机械压力后表现出相同的细胞行为模式。3)破骨细胞融合是破骨细胞主动吸收骨的一个过程，通过摄影记录。将肉豆蔻酸酯佛博尔（Phorbol Myristate acetate， PMA）通过微毛细管直接给药到破骨细胞上，证明了其活性的另一个过程。4)长期培养后，破骨细胞呈现退行性形态，最终被细胞周围的大相吞噬。