Generation of fatty liver with volatile chloro-compounds in adult rat hepatocytes in primary monolayer culture.

用苯巴比妥预处理大鼠肝细胞后，在美替拉酮存在下，细胞色素P-450在体内的含量保持在与肝脏相当的水平。研究了四氯化碳对细胞内甘油三酯周转率和酸性甘油三酯脂肪酶活性的影响。结果发现，培养基中前体的浓度对三酰甘油的合成、分泌和水解的时间过程没有影响，而四氯化碳显著抑制细胞内三酰甘油的水解和酸性脂肪酶的活性，同时三酰甘油也随之积累。提示四氯化碳在体外培养的大鼠肝细胞中引起的三酰甘油积累，除抑制其分泌外，还可能通过抑制溶酶体酸性脂肪酶来介导。

Pretreatment of rats with phenobarbital and plating the hepatocytes in the presence of metyrapone retained the content of cytochrome P-450 at the level equivalent to that in the liver in vivo.The effect of carbon tetrachloride on the turnover rate of the intracellular triacylglycerol and on the activity of acid triacylglycerol lipase was investigated in this experimental system of primary monolayer culture. It was found that the concentration of the precursors in the medium did not affect the time course of the synthesis, secretion, and hydrolysis of triacylglycerol, and that carbon tetrachloride significantly suppressed the hydrolysis of intracellular triacylglycerol and the activity of acid lipase with a concomitant accumulation of triacylglycerol.The result indicate a possibility that thetriacylglycerol accumulation in the cultured rat hepatocytes caused by carbon tetrachloride might be mediated by the inhibition of lysosomal acid lipase in addition to the suppression of the secretion.