Studies on the complement activation by human serum lectin.
人血清凝集素激活补体的研究。
基本信息
- 批准号:62570983
- 负责人:
- 金额:$ 1.28万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1987
- 资助国家:日本
- 起止时间:1987 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Human serum mannan-binding protein (MBP), which is a lectin specific for mannose and N-acetylglucosamine, was shown to lyse mannan-coated SRBC with the help of human complement. The activation by erum MBP was inhibited effectively by the presence of haptenic sugars and dependent absolutely upon the presence of C4, indicating that the actaivation is initiated by the sugar binding activity of MBP and proceeds through the classical pathway. ^<125>I-labeled C1r^^-_2s^^-_2 was shown to bind to MBP-mannan-SRBC complex regardless of the presence of C1q-depleted human complement had an ability to support to lyse SRBC sensitized with MBP, suggesting that MBP, once fixed on cell surfaces, functions as C1q does to initiate the activation of the classical pathway.The bacteria, rugh strains of Escherichia coli, K-12 and B, which had been sensitized with purified human serum MBP in the presence of CA^<2+>, followed by incubation with guinea pig complement, showed a marked decrease of colony forming … More ability compared with those not sensitized with the lectin. The bactericidal effect depended on the concentrations of the lectin and complement. The C4-dependency of the reaction indicated that the complement-dependent bactericidal action by MBP is expressed through the classical pathway. The bacteria were aggregated by MBP. Scatchard polt analysis of ^<125>I-labeled BMP binding to the bacteria showed that the dissociation constant (K_d) and the maximum binding capacity was 6x10<@1-9<@D1M and 30,000 molecules of MBP per a cell, respectively. The binding was inhibited by mannose, N-acetylglucosamine, suggesting that MBP recognized mannoheptose and N-acetylgucosamine constituting the rough core oligosaccharides of the bacterial cell wall.Thus, MBP-ligand (the bacteria) complex activates complement via the classical pathway possibly through the binding directly to C1r^^-_2s^^-_2 without the involvement of C1q and eventually the bacteria are killed. These findings demonstrate the physiological significance of the serum lectin in host defense, being consistent with the avirulence of E.coli rough strains in mammals. Less
人血清甘露聚糖结合蛋白 (MBP) 是一种甘露糖和 N-乙酰氨基葡萄糖特异性凝集素,已被证明可以在人补体的帮助下裂解甘露聚糖包被的 SRBC。血清 MBP 的激活被半抗原糖的存在有效抑制,并且完全依赖于 C4 的存在,表明激活是由 MBP 的糖结合活性引发的,并通过经典途径进行。 ^<125>I-标记的 C1r^^-_2s^^-_2 显示与 MBP-甘露聚糖-SRBC 复合物结合,无论是否存在 C1q 耗尽的人类补体,都能够支持裂解用 MBP 致敏的 SRBC,这表明 MBP 一旦固定在细胞表面上,就会像 C1q 一样发挥作用,启动经典途径的激活。细菌, 大肠杆菌K-12和B的rugh菌株在CA^<2+>存在下用纯化的人血清MBP致敏,随后与豚鼠补体一起孵育,与未用凝集素致敏的菌株相比,显示出集落形成能力显着降低。杀菌效果取决于凝集素和补体的浓度。反应的 C4 依赖性表明 MBP 的补体依赖性杀菌作用是通过经典途径表达的。细菌通过 MBP 聚集。 125 I标记的BMP与细菌结合的Scatchard polt分析表明,解离常数(K_d)和最大结合容量分别为6x10<@1-9<@D1M和每个细胞30,000个MBP分子。该结合被甘露糖、N-乙酰氨基葡萄糖抑制,表明MBP识别构成细菌细胞壁的粗糙核心寡糖的甘露庚糖和N-乙酰氨基葡萄糖。因此,MBP-配体(细菌)复合物可能通过直接与C1r^^-_2s^^-_2结合而通过经典途径激活补体,而无需 C1q 的参与,最终细菌被杀死。这些发现证明了血清凝集素在宿主防御中的生理意义,与哺乳动物中大肠杆菌粗菌株的无毒力一致。较少的
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toshisuke Kawasaki: "Isolation of mannose/N-acetylglucosamine binding proteins from mammalian sara." Methods in Enzymology. in press. (1989)
Toshisuke Kawasaki:“从哺乳动物 sara 中分离甘露糖/N-乙酰氨基葡萄糖结合蛋白。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshisuke Kawasaki: "Serum mannan-binding protein (s-MBP) activates complement through the classical pathway." Proceedings of the IXth International Symposium on Glycoconjugates.62 (1987)
Toshisuke Kawasaki:“血清甘露聚糖结合蛋白(s-MBP)通过经典途径激活补体。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ken Ikeda: "Serum lectin with known structure activates complement through the classical pathway." The Journal of Biological Chemistry. 262. 7451-7454 (1987)
Ken Ikeda:“结构已知的血清凝集素通过经典途径激活补体。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshisuke Kawasaki.: Proceedings of the IXth International Symposium on Glicoconjugates.G-62 (1987)
Toshisuke Kawasaki.:第九届糖复合物国际研讨会论文集.G-62 (1987)
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- 影响因子:0
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KAWASAKI Nobuko其他文献
KAWASAKI Nobuko的其他文献
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{{ truncateString('KAWASAKI Nobuko', 18)}}的其他基金
Characterization and physiological significance of the interaction between mannan-binding protein and matrix metalloproteases.
甘露聚糖结合蛋白与基质金属蛋白酶之间相互作用的表征和生理意义。
- 批准号:
20590074 - 财政年份:2008
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Novel Carbohydrate Ligands for a Serum Lectin Expressed on Colon Cancer Cells and Associated with Anti-tumor Activity
在结肠癌细胞上表达并与抗肿瘤活性相关的血清凝集素的新型碳水化合物配体
- 批准号:
18590053 - 财政年份:2006
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Characterization of Novel Carbohydrate Ligands for Serum Lectin Associated with Anti-tumor Activity
与抗肿瘤活性相关的血清凝集素新型碳水化合物配体的表征
- 批准号:
16590046 - 财政年份:2004
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Structural analysis of oligosaccharides ligands to a serum lectin inducing an anti-tumor activity
诱导抗肿瘤活性的血清凝集素寡糖配体的结构分析
- 批准号:
14572054 - 财政年份:2002
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Regulation of the Serum Level of the Collectins Associated with Host Defense
与宿主防御相关的集合素血清水平的调节
- 批准号:
09672223 - 财政年份:1997
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Gene expression control of animal lectins containing a collagen-like domain.
含有胶原蛋白样结构域的动物凝集素的基因表达控制。
- 批准号:
07672354 - 财政年份:1995
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Gene structure of serum lectins containing a collagen-like domain.
含有胶原蛋白样结构域的血清凝集素的基因结构。
- 批准号:
05671817 - 财政年份:1993
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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NMR BASED SCREENING FOR NEW CARBOHYDRATE BINDING PROTEIN LIGANDS
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