Hematinic acid produced by the reaction of phenylhemoglobin with hydrogen peroxide

苯血红蛋白与过氧化氢反应生成苏木酸

• 批准号: 62570988
• 负责人: HIROTA Kazuhiro
• 金额: $ 1.34万
• 依托单位: Health Sciences of Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570988/
• 关键词: phenylhydrazine; hematinic acid; hemoglobin heme; ヘモグロビン; ガスクロマトグラフィー; 赤血球; ピロール

It is proposed that phenylhydrazine precipitates altered hemoglobin known as Heinz bodies in erythroxytes and that the life span of erythrocytes in the circulation is shortened by the presence of these precipitates. However, the formation mechanism of Heinz bories is not fully understand. Phenylhemoglobin, formed by the reaction of hemoglobin with phenylhydrazine, is expected to be a destabilized molecule susceptive to oxidation with oxidants concomitantly formed in the reaction between hemoglobin and oxygen.Oxidation of phenylhemoglobin with hydrogen peroxide was studied in this paper. Hematinic acid as an oxidative product of the hemoglobin heme was detected by gas chromatographic analysis after the conversion of methyl ester of the compound. Accelerated production of the compound was observed with increasing concentration of hydrogen peroxide. The results showed that heme group of phenylhemoglobin was susceptive to oxidant suchas hydrogen peroxide. which is associated with hemolysis induced by Heinzbodies.

有人提出，苯肼沉淀改变血红蛋白称为海因茨小体在红细胞和红细胞在循环中的寿命缩短这些沉淀物的存在。然而，海因茨硼化物的形成机理还不完全清楚。由血红蛋白与苯肼反应生成的苯基血红蛋白是一种不稳定的分子，它对氧与血红蛋白反应时生成的氧化剂具有抵抗氧化的能力，本文研究了过氧化氢氧化苯基血红蛋白的反应。血红素酸作为血红蛋白血红素的氧化产物，在该化合物的甲酯转化后通过气相色谱分析检测。随着过氧化氢浓度的增加，观察到该化合物的加速产生。结果表明，苯基血红蛋白中的血红素基团对过氧化氢等氧化剂有一定的抵抗性。其与亨氏体诱导的溶血有关。