Hematinic acid produced by the reaction of phenylhemoglobin with hydrogen peroxide

有人认为，苯肼沉淀了红细胞中称为亨氏小体的改变的血红蛋白，这些沉淀物的存在缩短了红细胞在循环中的寿命。然而，海因茨·博利的形成机制还没有完全弄清楚。苯基血红蛋白是由血红蛋白与苯肼反应生成的一种不稳定分子，在血红蛋白与氧的反应中伴随着氧化剂的生成，是一种易氧化的不稳定分子。化合物的甲酯转化后，用气相色谱分析检测到血红蛋白血红素的氧化产物血酸。随着过氧化氢浓度的增加，该化合物的生成速度加快。结果表明，苯基血红蛋白中的血红素基团对过氧化氢等氧化剂敏感。这与海因茨小体引起的溶血有关。

It is proposed that phenylhydrazine precipitates altered hemoglobin known as Heinz bodies in erythroxytes and that the life span of erythrocytes in the circulation is shortened by the presence of these precipitates. However, the formation mechanism of Heinz bories is not fully understand. Phenylhemoglobin, formed by the reaction of hemoglobin with phenylhydrazine, is expected to be a destabilized molecule susceptive to oxidation with oxidants concomitantly formed in the reaction between hemoglobin and oxygen.Oxidation of phenylhemoglobin with hydrogen peroxide was studied in this paper. Hematinic acid as an oxidative product of the hemoglobin heme was detected by gas chromatographic analysis after the conversion of methyl ester of the compound. Accelerated production of the compound was observed with increasing concentration of hydrogen peroxide. The results showed that heme group of phenylhemoglobin was susceptive to oxidant suchas hydrogen peroxide. which is associated with hemolysis induced by Heinzbodies.