Aging of Erythrocytes Induced by the Oxidation of Hemoglobin Heme

血红蛋白血红素氧化引起的红细胞衰老

• 批准号: 01571209
• 负责人: HIROTA Kazuhiro
• 金额: $ 0.32万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571209/
• 关键词: hemoglobin; heme; protoporphyrin; hematinic acid; oxidation; ヘモグロビン; 赤血球

Phenylhydrazine is an especially effective chemical inducer of hemolysis. It is well established that phenylhydrazine results in precipitates of altered hemoglobin known as Heinz bodies in erythrocytes and the life span of erythrocytes in the circulation is shortened by the presence of these precipitates. The formation of precipitation, which has been studied on the base of the oxidative alternation and following denaturation of hemoglobin, is not fully understood. Among the reactions postulated to have important role in the oxidative denaturation of hemoglobin by phenylhydrazine are oxidation of porphyrin, oxidation of thiols on globins, formation of ferrihemoglobin, formation of endogeneously liganded globin ferrihemochrome, and formation of phenylheme in hemoglobin.In this paper, we revealed that phenylhydrazine was reacted with human hemoglobin and produced hematinic acid (monopyrrole) which is a products of oxidative cleavage of porphyrin. Mechanism of hemolysis induced by phenylhydrazine was considered on the basis of the cleavage ability of porphyrin in hemoglobin by phenylhydrazine.

苯肼是一种特别有效的溶血化学诱导剂。众所周知，苯肼会在红细胞中产生称为亨氏小体的改变的血红蛋白沉淀物，这些沉淀物的存在会缩短红细胞在循环中的寿命。沉淀的形成一直是基于血红蛋白的氧化交替和随后的变性来研究的，但还不完全清楚。在苯肼对血红蛋白的氧化变性中起重要作用的反应有：卟啉的氧化、硫醇在珠蛋白上的氧化、铁血红蛋白的形成、内源性连接的珠蛋白铁色素的形成以及血红蛋白中苯基血红素的形成。根据苯肼对血红蛋白中的卟啉的裂解能力，探讨了苯肼诱导溶血的机理。

项目成果

廣田 和弘: "Formation of hematinic acid as a degradative product of heme by the reaction of hemoglobin with phenylhydrazine or hydrogen peroxide" Anchives of Biochemistry and Biophysics. (1991)

Kazuhiro Hirota：“血红蛋白与苯肼或过氧化氢反应形成血红素降解产物”Achives of Biochemistry and Biophysicals (1991)。

廣田 和弘: "赤血球中のヘモグロビンの酸化開裂物質ヘマチン酸の定量" 岡山大学医療技術短期大学部紀要. 1. (1991)

Kazuhiro Hirota：“红细胞中血红蛋白氧化裂解物质血酸的测定”冈山大学医学技术学院通报1。（1991）。

廣田 和弘,佐々木 健二,廣田 喬: "赤血球中のヘモグロビンの酸化開裂物質へマチン酸の定量" 岡山大学医療技術短期大学部紀要. 1. 77-82 (1991)

Kazuhiro Hirota、Kenji Sasaki、Takashi Hirota：“红细胞中血红蛋白氧化裂解物质血酸的测定”冈山大学医学技术学院通报1. 77-82（1991）。

K. Hirota, T. Hatanaka and T. Hirota: "Isolation of N-phenylprotoporphyrin IX from the red cells and spleen of the phenylhydrazine- treated rat" Arch. of Bio chem. and Bio phy.255. 42-47 (1987)

K. Hirota、T. Hatanaka 和 T. Hirota：“从苯肼处理的大鼠的红细胞和脾脏中分离 N-苯基原卟啉 IX”Arch。

廣田 和弘: "Fomation of hematinic acid as a degradative product of heme by the reaction of hemoglobin with phenylhydrazine or hydrogen peroxide" Archives of Biochemistry and Biophysics. (1991)

Kazuhiro Hirota：“血红蛋白与苯肼或过氧化氢反应形成血红素降解产物”，生物化学和生物物理学档案（1991）。