Functional regulation of cultured vascular endothelial cells.

培养的血管内皮细胞的功能调节。

基本信息

批准号：
62571006
负责人：
YAMAMOTO K.
金额：
$ 1.28万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62571006/
关键词：
Isolation and cell culture vascular endothelial cells Expression of the function Functional changes in vitro cellular aging 血管内皮細胞の長期継代培養毛細血管内皮細胞の培養法染色体異常分化機能

项目摘要

1. We were established various vascular endothelial cell lines from animals (bovine, porcine, and rabbit) and human (umbilical veins, umbilical artery, adult arteries, adult veins, and omentum microvessels). 2. We characterized these vascular endothelial cells as to cell growth and Prostacyclin (PGI_2) production. ECGF and basic FGF stimulated human umbilical vein endothelial (HUVE) cell growth. Heparin further enhanced the cell growth stimulated by ECGF, but not the cell growth stimulated by FGF or in the absence of these growth factors. In the presence of ECGF, the PGI_2-producing capacity of the cells was inhibited by heparin. Heparin-treated cultures may not be suitable for some examinations of PGI_2 production by vascular endothelial cells. HUVE cells produced 2-fold (based on cell number) or 3-fold (based on unit area) more PGI_2 in the stationary phase than in the growing phase. 3. We investigated some properties of human vascular endothelial cells and bovine arotic endothelial cells during in vitro aging. Cell growth potential and cell density decreased gradually in the process of in vitro aging. Cell volume and occurrence of multinucleated cells increased with cellular aging. PGI_2 production and negative charge of the cell surface decreased progressively during in vitro cellular aging. Factor VIII-related antigen were expressed in the cells throughout the culture period. The changes observed were consistent with the previously described changes in vascular endothelial cells during in vivo aging. Therefore, we conclude that cumulative cell division is deeply involved in in vivo aging of endothelial cells and that this in vitro system is suitable for investigating in vivo aging of vascular endothelial cells.

1。我们建立了来自动物（牛，猪和兔子）和人类（脐静脉，脐动脉，成年动脉，成年静脉和Omentum微量无梭）的各种血管内皮细胞系。 2。我们将这些血管内皮细胞表征为细胞生长和前列环蛋白（PGI_2）的产生。 ECGF和基本FGF刺激了人脐静脉内皮（HUVE）细胞生长。肝素进一步增强了ECGF刺激的细胞生长，而不是在没有这些生长因子的情况下刺激的细胞生长。在存在ECGF的情况下，肝素抑制了细胞的PGI_2产生能力。肝素处理的培养物可能不适合通过血管内皮细胞生产PGI_2的某些检查。 Huve细胞在固定阶段产生的PGI_2比在生长阶段中产生的PGI_2多2倍（基于单位面积）。 3。我们研究了人血管内皮细胞和牛植物内皮细胞的某些特性。在体外衰老的过程中，细胞生长潜力和细胞密度逐渐降低。细胞体积和多核细胞的发生随细胞衰老而增加。在体外细胞衰老期间，PGI_2的产生和细胞表面负电荷逐渐降低。在整个培养期内，在细胞中表达了与VIII因子相关因子相关的因子。观察到的变化与体内老化期间血管内皮细胞的先前描述的变化一致。因此，我们得出的结论是，累积细胞分裂与内皮细胞的体内衰老深远有关，并且该体外系统适合研究血管内皮细胞体内老化的体内。