Signal Transmittance on Interferon-gamma-Treated Murine Immunocompetent Cells

IFN-γ是已知的巨噬细胞活化因子之一。我们通过使用各种参数检测了IFN-γ对小鼠腹腔巨噬细胞的作用。结果如下：1. IFN-γ不增加细胞膜通透性、Ca^2+浓度、二乙酰甘油酯、中性脂质和蛋白激酶C活性。2. IFN-γ对细胞内的pH值和Na^+浓度没有任何影响。3. IFN-γ抑制细胞中腺苷酸环化酶的活性。4. IFN-γ增加宿主对沙门氏菌和嗜肺军团菌的抗性。同时给予小鼠IFN-γ和TNF-α比任何一种都能增强抵抗力。

IFN-gamma is known one of the macrophage activating factors. We examined the effect of IFN-gamma on murine peritoneal macrophages by using various parameters. The results were as follows;1. IFN-gamma did not increase the membrane permeability, Ca^<2+> concentration, diacetylglyceride, neutral lipid and proteinkinase C activity in the cells. 2. IFN-gamma did not affect any pH and Na^+ concentration in the cells. 3. IFN-gamma suppressed the activity of adenylcyclase in the cells. 4. IFN-gamma increased the host resistance against Salmonella- and Legionella pneumophila. Simultaneous administration of IFN-gamma and TNF-alpha into mice enhanced more resistance than that of either one.