Signal Transmittance on Interferon-gamma-Treated Murine Immunocompetent Cells

干扰素γ处理的鼠免疫活性细胞的信号透射率

• 批准号: 63044127
• 负责人: NAKANO Masayasu
• 金额: $ 3.84万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Survey.
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044127/
• 关键词: interferon-gamma; mouse; immunecompetent cells; intracellular signal transmission; interleukin-1; macrophages; adenylate cyclase; protein-kinase C

IFN-gamma is known one of the macrophage activating factors. We examined the effect of IFN-gamma on murine peritoneal macrophages by using various parameters. The results were as follows;1. IFN-gamma did not increase the membrane permeability, Ca^<2+> concentration, diacetylglyceride, neutral lipid and proteinkinase C activity in the cells. 2. IFN-gamma did not affect any pH and Na^+ concentration in the cells. 3. IFN-gamma suppressed the activity of adenylcyclase in the cells. 4. IFN-gamma increased the host resistance against Salmonella- and Legionella pneumophila. Simultaneous administration of IFN-gamma and TNF-alpha into mice enhanced more resistance than that of either one.

IFN-γ是已知的巨噬细胞活化因子之一。我们通过使用各种参数检测了IFN-γ对小鼠腹腔巨噬细胞的作用。结果如下：1. IFN-γ不增加细胞膜通透性、Ca^2+浓度、二乙酰甘油酯、中性脂质和蛋白激酶C活性。2. IFN-γ对细胞内的pH值和Na^+浓度没有任何影响。3. IFN-γ抑制细胞中腺苷酸环化酶的活性。4. IFN-γ增加宿主对沙门氏菌和嗜肺军团菌的抗性。同时给予小鼠IFN-γ和TNF-α比任何一种都能增强抵抗力。

项目成果

Terada,Y.: "Defect of calmodulin-binding protein in expression of interleukin 1 gene by LPS-nonresponder C3H/HeJ mouse macrophages." Biochemical and Biophysical Research Communication. 158. 723-729 (1989)

Terada,Y.：“LPS 无反应 C3H/HeJ 小鼠巨噬细胞表达白细胞介素 1 基因时钙调蛋白结合蛋白的缺陷。”

Blanchard,D.K.: "Induction of interferon-gamma and tumor necrosis factor by Legionella pneumophila:Augmentation of human neutrophil bactrericidal activity." Journal of Leukocyte Biology. 45. 538-549 (1989)

Blanchard，D.K.：“嗜肺军团菌诱导干扰素-γ 和肿瘤坏死因子：增强人中性粒细胞杀菌活性。”

M.S. Shamma: "The mode of PGE2-induced desensitization of adenylate cyclase of a murine macrophage-like cell line (P388d1)" Prostaglandins. 36. 329-341 (1988)

多发性硬化症。

Matteucci, D.: "Interleukin 1 and 2 production and responsiveness in the early stages of retroviral infections of mice." Cellular Immunology. 119. 402-411 (1989)

Matteucci, D.：“小鼠逆转录病毒感染早期阶段白细胞介素 1 和 2 的产生和反应。”

Matsumura, H.: "Effect of murine recombinant interferon-gamma in the protection of mice against Salmonella." International Journal of Immunopharmacology. 12. 49-65 (1990)

Matsumura, H.：“鼠重组干扰素-γ 在保护小鼠免受沙门氏菌侵害方面的作用。”