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Gross culture system for cyanobacteria & its application for medication

蓝藻粗培养系统

基本信息

批准号：
03557024
负责人：
NAKANO Masayasu
金额：
$ 10.94万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

A gross culture system for microcystis (cyanobactera) has been developed. When the cells of Microcystis aeruginosa K-139 strain were inoculated (10% v/v) into the system with 10 litters of CB medium (pH 10.0) and were cultured at 28C。 under the conditions of airing (filter-passed air, 9 liters/min) and lighting (4 directions, cool white light, 10, 000 lux). The pH of medium was maintained between 9 and 10, by being adjusted with 1 N HCl solution during the culture period. The cell-growth reached the maximum at day 7, and the yield of cells was 4.56 g. It was 2.5 times greater than that obtained from a stationary culture. The yield of microcystin extracted from the cell-mass was 27.493 mg, and it was 3 times more than that of the statinary culture.Monoclonal antibodies against microcystin-LR were prepared from hybridoma cell lines. All the antibodies were reacted with not only microcystin-LR but also other microcystins tested. Microcystin-shock in mice could hardly be protected by a previous administration of these antibodies. The microcystin-shock was relieved to some extent when interleukin (IL)-10 was injected 30 min in advance. The serum-level of IL-6 induced by microcystin was lowered by a previous injection of IL-10.The cell-extract of Microcystis aeruginosa K-18 strain contained anti-bacterial activity against various kinds of gram-positive and negative bacteria. The active substance was purified. However, it was found to be gamma-linolenic acid.

建立了微囊藻(蓝藻)的整体培养系统。将铜绿微囊藻K-139菌株的细胞接种到含有10升CB(pH 10.0)的培养液中，在28℃下培养。在通风(过滤器通过的空气，9升/分钟)和照明(4个方向，冷白光，10,000勒克斯)的条件下。在培养过程中，用1N的HCl溶液调节培养基的pH值，使其保持在9~10之间。细胞生长在第7天达到最大值，细胞产量为4.56g，是静止培养的2.5倍。从菌体中提取的微囊藻毒素产量为27.493 mg，是原发酵液的3倍。所有抗体不仅与微囊藻毒素-LR反应，还与其他微囊藻毒素反应。小鼠的微囊藻毒素休克几乎不能通过以前注射这些抗体来保护。提前30min注射IL-10可在一定程度上缓解微囊藻毒素休克。预先注射IL-10可降低微囊藻毒素诱导的血清IL-6水平。铜绿微囊藻K-18菌株细胞提取物对各种革兰氏阳性菌和阴性菌均有抗菌活性。对活性物质进行了纯化。然而，它被发现是伽马亚麻酸。