Gross culture system for cyanobacteria & its application for medication

开发了微囊藻（蓝藻）粗培养系统。将铜绿微囊藻K-139株细胞以10% v/v接种于10窝CB培养基（pH 10.0）中，28℃培养。通风（过滤通过空气，9升/分钟）和照明（4个方向，冷白光，10000勒克斯）条件下。培养基在培养过程中，通过加入1n HCl溶液调节，pH值保持在9 ~ 10之间。细胞生长在第7天达到最大值，细胞产量为4.56 g。它是固定培养的2.5倍。从细胞团中提取的微囊藻毒素的产量为27.493 mg，是固定培养的3倍。从杂交瘤细胞系制备了微囊藻毒素- lr单克隆抗体。所有抗体不仅与微囊藻毒素- lr反应，而且与其他被测微囊藻毒素反应。小鼠的微囊藻毒素休克很难被先前的这些抗体所保护。提前30min注射白细胞介素-10可在一定程度上缓解微囊毒素休克。微囊藻毒素诱导的血清IL-6水平通过先前注射IL-10而降低。铜绿微囊藻K-18株细胞提取物对多种革兰氏阳性和阴性菌均有抑菌活性。活性物质被纯化。然而，它被发现是-亚麻酸。

A gross culture system for microcystis (cyanobactera) has been developed. When the cells of Microcystis aeruginosa K-139 strain were inoculated (10% v/v) into the system with 10 litters of CB medium (pH 10.0) and were cultured at 28C。 under the conditions of airing (filter-passed air, 9 liters/min) and lighting (4 directions, cool white light, 10, 000 lux). The pH of medium was maintained between 9 and 10, by being adjusted with 1 N HCl solution during the culture period. The cell-growth reached the maximum at day 7, and the yield of cells was 4.56 g. It was 2.5 times greater than that obtained from a stationary culture. The yield of microcystin extracted from the cell-mass was 27.493 mg, and it was 3 times more than that of the statinary culture.Monoclonal antibodies against microcystin-LR were prepared from hybridoma cell lines. All the antibodies were reacted with not only microcystin-LR but also other microcystins tested. Microcystin-shock in mice could hardly be protected by a previous administration of these antibodies. The microcystin-shock was relieved to some extent when interleukin (IL)-10 was injected 30 min in advance. The serum-level of IL-6 induced by microcystin was lowered by a previous injection of IL-10.The cell-extract of Microcystis aeruginosa K-18 strain contained anti-bacterial activity against various kinds of gram-positive and negative bacteria. The active substance was purified. However, it was found to be gamma-linolenic acid.