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Regulatory Factors of Morphogenesis

形态发生的调控因素

基本信息

批准号：
63304007
负责人：
EGUCHI Goro
金额：
$ 11.58万
依托单位：
Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Following results were obtained in this fiscal year which completes this project.1. We found a regulatory factor of tissue regeneration in the newt, and further discovered that this factor has a direct influence on the destabilization of cells in the course of lens and limb regeneration. A basic technique of purification of this factor was established.2. We showed that qualitative and quantitative regulation of the expression of cadherin molecules is one of the important processes which direct the morphogenesis of tissues and organs in the course of embryonic development.3. We have firstly identified activen as a fundamental regulatory factor of the mesodermal induction, and analyzed its mode of action.4. A monoclonal antibody which recognizes a receptor of nerve-inducing-substance in amphibia was isolated as a clue for the mechanisms of neural induction.5. AV-1 protein, which concerns the pattern formation of bones in chicken limb bud, was shown to be distinctly expressed in different bones. We have also succeeded in the purification of AV-1 from 60,000 pieces of limb buds.6. Molecular basis of the regulation of the formation of optic nerve network was founded through identification of A5 and B2 molecules, both of which acts competitively.7. An effective assay system for regulatory factors of morphogenesis in hydra was established.Proposals at the onset of our project have been almost completed and new perspectives for researches of morphogenesis at higher orders were obtained.

完成该项目的本财年取得了以下成果： 1．我们在蝾螈身上发现了一个组织再生的调节因子，并进一步发现这个因子对晶状体和肢体再生过程中细胞的不稳定有直接影响。建立了该因子纯化的基本技术。 2．我们发现钙粘蛋白分子表达的定性和定量调控是胚胎发育过程中指导组织器官形态发生的重要过程之一。 3．我们首先确定了activen是中胚层诱导的基本调控因子，并分析了其作用机制。 4．分离出识别两栖动物神经诱导物质受体的单克隆抗体，为神经诱导机制的研究提供线索。 5． AV-1蛋白与鸡肢芽骨骼的形成模式有关，在不同的骨骼中具有明显的表达。我们还成功地从6万片肢芽中纯化出AV-1。6．通过对竞争性作用的A5和B2分子的鉴定，建立了视神经网络形成调控的分子基础。 7．建立了水螅形态发生调控因子的有效检测体系。项目启动之初的提案已基本完成，为高阶形态发生的研究提供了新的视角。