Regulatory Factors of Morphogenesis

形态发生的调控因素

基本信息

批准号：
63304007
负责人：
EGUCHI Goro
金额：
$ 11.58万
依托单位：
Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Following results were obtained in this fiscal year which completes this project.1. We found a regulatory factor of tissue regeneration in the newt, and further discovered that this factor has a direct influence on the destabilization of cells in the course of lens and limb regeneration. A basic technique of purification of this factor was established.2. We showed that qualitative and quantitative regulation of the expression of cadherin molecules is one of the important processes which direct the morphogenesis of tissues and organs in the course of embryonic development.3. We have firstly identified activen as a fundamental regulatory factor of the mesodermal induction, and analyzed its mode of action.4. A monoclonal antibody which recognizes a receptor of nerve-inducing-substance in amphibia was isolated as a clue for the mechanisms of neural induction.5. AV-1 protein, which concerns the pattern formation of bones in chicken limb bud, was shown to be distinctly expressed in different bones. We have also succeeded in the purification of AV-1 from 60,000 pieces of limb buds.6. Molecular basis of the regulation of the formation of optic nerve network was founded through identification of A5 and B2 molecules, both of which acts competitively.7. An effective assay system for regulatory factors of morphogenesis in hydra was established.Proposals at the onset of our project have been almost completed and new perspectives for researches of morphogenesis at higher orders were obtained.

在本财政年度完成了以下结果，该项目完成了该项目1。我们发现了NEWT组织再生的调节因素，并进一步发现，该因子在晶状体和肢体再生过程中直接影响细胞的不稳定。建立了一种纯化的基本技术。2。我们表明，钙粘蛋白分子表达的定性和定量调节是指导胚胎发育过程中组织和器官形态发生的重要过程之一。3。我们首先将ARCIVEN确定为中胚层诱导的基本调节因素，并分析了其作用方式。4。单克隆抗体识别两栖动物中神经诱导替代的受体被分离为神经诱导机制的线索。5。 AV-1蛋白涉及鸡肉肢芽中骨骼的图案形成，被证明在不同的骨骼中明显表达。我们还成功地从60,000块肢体芽中纯化了AV-1。通过鉴定A5和B2分子的鉴定，建立了视神经网络形成的分子基础，这两种分子均具有竞争性的作用。7。建立了一种有效的调节性因素，建立了九头蛇的形态发生因素。在我们的项目开始时，几乎已经完成了proposals，并获得了更高阶的形态发生研究的新观点。