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Developmental Physiological Research of Intracellular Dynamic Behaviors of Calcium Ion.

钙离子细胞内动态行为的发育生理学研究。

基本信息

批准号：
63304006
负责人：
HIRAMOTO Yukio
金额：
$ 12.22万
依托单位：
The University of the Air
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63304006/
关键词：
Calcium ion Fertilization Amoeboid movement Sea urchin egg Medaka egg Hamster egg Neuron カルシウムイオンカルシウムエクオリンアメーバ運動原形質流動 Fura2

项目摘要

Roles of calcium ions in maturation, fertilization and cleavage of egg cells, amoeboid movement, protoplasmic streaming, and in excitation of neurons were investigated as follows. 1. A transient increase in intracellular calcium ions was detected in maturation of the starfish oocyte. 2. Simultaneous observation of morphological changes by differential interference microscopy and changes in calcium ion concentration with fluorescent microscopy in sea urchin eggs injected with fluorescent calcium indicator revealed relations between the morphological change and intracellular calcium changes in eggs upon fertilization. 3. Using artificial lipid membrane embedded with membrane fractions isolated from sea urchin eggs which release calcium ions by application of ATP, presence of Ca-, K- and Cl-ion channels in the sea urchin egg membrane was found. 4. A transient increase in calcium ions in newt eggs upon fertilization was shown by the luminescence of aequorin microinjected into the egg. 5. Upon fertilization in hamster eggs. the activation of GTP-binding protein (S), production of inositol triphosphate and release of calcium ions were found. 6. Temporal relationship between intracellularcalcium ions and various events from the attachment of sperm to the egg surface through the breakdown of cortical alveoli was clarified in medaka eggs. 7. Intracellular calciumion concentration rhythmically changed in parallel with cleavage cycle in Xenopus eggs. 8. Intracellular calcium ion concentrat ion in amoeba repeatedly increased at the site of solーgel change near its tail. 9. A new technique to determine calcium ion concentration in cells and tissues was devised. Using this technique, calcium ion concentrations in neurons and at the synaptic regions were determined.

本文探讨了钙离子在卵细胞成熟、受精和分裂、变形虫运动、原生质流动和神经元兴奋中的作用。1. 在海星卵母细胞成熟过程中发现细胞内钙离子的短暂增加。2. 用差示干涉显微镜观察注射荧光钙指示剂的海胆卵的形态变化，并用荧光显微镜观察钙离子浓度的变化，揭示了受精后海胆卵形态变化与细胞内钙变化之间的关系。3. 采用人工脂膜包埋海胆卵分离膜组分，通过ATP释放钙离子，发现海胆卵膜中存在Ca-、K-和cl -离子通道。4. 在蝾螈卵中微量注射水芹蛋白，结果显示受精后卵中钙离子的短暂增加。5. 在仓鼠卵中受精。gtp结合蛋白(S)活化，三磷酸肌醇生成，钙离子释放。6. 细胞内钙离子与精子通过皮层泡破裂附着到卵子表面的各种事件之间的时间关系得到了澄清。7. 爪蟾卵细胞内钙离子浓度与卵裂周期平行发生节律性变化。8. 变形虫细胞内钙离子浓度在其尾部附近的溶胶凝胶变化部位反复升高。9. 提出了一种测定细胞和组织中钙离子浓度的新方法。利用这种技术，测定了神经元和突触区域的钙离子浓度。