Trial manufacture of an ultra-high sensitive video-microscope system with and image-processor and its applications to cell biology

开发了一种视频显微镜系统，以便在极低强度的光下对微观物体进行可视化。利用该系统，在海胆、海星、青蛙、青蛙和金黄地鼠的卵中，记录了受精和人工激活时细胞内钙离子的释放。在这些卵子中，细胞内钙离子浓度的增加始于精子进入或人工激活的位置，并通过细胞皮质向对足目动物传播。这一钙波先于皮质肺泡或皮质颗粒的破裂波，表明钙离子的增加诱导了皮质的变化。在青竹受精和海胆卵中，细胞内钙离子伴随细胞周期的周期性变化被记录下来。在这两个卵子中，细胞内钙离子浓度在卵裂过程中都达到了最低值。用aequorin作为探针，记录了伴随着阿瓦类运动和黏菌中原生质流的细胞内钙离子的短暂和局限性的增加。用钙离子的荧光探针Fura 2记录了细胞内钙离子浓度的变化。

A video-microscope system was developed, in order to visualize microscopic objects under an extremely-low intensity light. Using this system, intracellular release of calcium ions at fertilization and artifical activation was recorded in eggs of sea urchin, starfish, medaka, frog and golden hamster, which had been injected with aequorin, a photoprotein sensitive to free calcium ions. In these eggs, the increase in intracellular calcium ion concentration was started at the site of sperm entry or the site of artificial activation and it propagated over the cell cortex toward the antipod. This calcium wave preceded the wave of the breakdown of cortical alveoli or cortical granules, suggesting that the increase in calcium ions induces the cortical change.Periodic changes in intracellular calcium ions accompanying cell cycle were recorded in fertilized medaka and sea urchin eggs injected with aequorin. In both eggs, intracellular calcium ion concentration attained a minimum during cleavage.Transient and localized increases in intracellular calcium ions accoumpanying amoevoid movement and protoplasmic streaming in slime mold were recorded using aequorin as a probe.Changes in intracellular calcium concentration were also recorded using fura 2, a fluorescent probe for calcium ions.