Molecular Diversity and Physiological Roles of Growth Inhibitors
生长抑制剂的分子多样性和生理作用
基本信息
- 批准号:63304060
- 负责人:
- 金额:$ 8.96万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Co-operative Research (A)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Considerable evidence has accumulated indicating that proliferation of animal cells is regulated by a balance between positive and negative growth stimuli. To clarify molecular species and physiological roles of growth inhibitors, each member of this research project has studied cytostatic or cytotoxic proteins present in different sources such as sera and cultured cells. Both cytostatic and cytotoxic factors were purified and characterized from normal rabbit serum. The former was a heat-and acid-labile but dithiothreitol-resistant protein with 56 kDa, which reversibly inhibited the growth of various malignant and nonmalignant cells. The latter existed in a latent form in the serum and was activated by incubating the serum at 37 ^OC for 12 h. Two active forms with 18 kDa and 36 kDa were purified from the incubated serum. Both proteins, which were heat- and acid-labile but dithiothreitol-resistant, exerted a strong cytolytic effect on rodent cells regardless of cell type and malignancy. Other growth inhibitors characterized in this study include mouse neonatal brain-derived carcinostatic factor (NBCF), tumor-degenerating factor (TDF) secreted by human fibroblasts, neuroblast growth inhibitory factor (NGIF) secreted by rat glioblasts, and tumor-regressing factor (TRF) induced by antitumor polysaccharides in the serum of tumor-bearing mice.
大量证据表明,动物细胞的增殖受到正负向生长刺激之间的平衡调节。为了阐明生长抑制物的分子种类和生理作用,该研究项目的每个成员都研究了存在于不同来源如血清和培养细胞中的细胞抑制蛋白或细胞毒蛋白。从正常兔血清中分离纯化细胞抑制因子和细胞毒因子,并对其进行鉴定。前者是一种耐热、耐酸、抗二硫苏糖醇的蛋白,大小为56 kDa,对多种恶性和非恶性细胞的生长具有可逆性抑制作用。后者以潜伏形式存在于血清中,37℃孵育12h可被激活,从孵育的血清中纯化出两种活性形式,分别为18 kDa和36 kDa。这两种蛋白质对热和酸不稳定,但对二硫苏糖醇耐受,无论细胞类型和恶性程度如何,对啮齿动物细胞都有很强的细胞溶解作用。本研究中表征的其他生长抑制因子包括小鼠新生脑源性抑癌因子(NBCF)、人成纤维细胞分泌的肿瘤退化因子(TDF)、大鼠成胶质细胞分泌的神经母细胞生长抑制因子(NGIF)以及荷瘤小鼠血清中抗肿瘤多糖诱导的肿瘤退化因子(TRF)。
项目成果
期刊论文数量(104)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Matsuyama: J.Biol.Chem.263. 17117-17121 (1988)
K.Matsuyama:J.Biol.Chem.263。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
宮崎 香: "TGFーαとTGFーβの生理的役割" 臨床免疫ー特集「Paracrine Aurocrine増殖因子」. 22. 541-550 (1990)
宫崎薰:“TGF-α 和 TGF-β 的生理作用”临床免疫学专题“旁分泌极分泌生长因子”22. 541-550 (1990)。
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- 影响因子:0
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K.Kageyama: "Enhanced inhibition of DNA synthesis and release of membrane phosphoーlipid in tumor cells treated with a combination of acylated ascorbate and hyperthermia." Int.J.Hyperthermia. 7. 85-91 (1991)
K.Kageyama:“结合酰化抗坏血酸和热疗增强对肿瘤治疗细胞中 DNA 合成和膜磷脂释放的抑制。”Int.J.Hyperthermia。
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- 影响因子:0
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MIYAZAKI Kaoru其他文献
MIYAZAKI Kaoru的其他文献
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{{ truncateString('MIYAZAKI Kaoru', 18)}}的其他基金
Interaction of cancer cells with basement membrane proteins
癌细胞与基底膜蛋白的相互作用
- 批准号:
17014077 - 财政年份:2005
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Application of a new cell-adhesive and cell-migatory molecule, laminin-5B, to regenerative medicine
新型细胞粘附和细胞迁移分子层粘连蛋白-5B在再生医学中的应用
- 批准号:
17300157 - 财政年份:2005
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structure and Function of Laminin-5, a Cell Adhesion Protein of Basement Membrane
基底膜细胞粘附蛋白Laminin-5的结构与功能
- 批准号:
11490030 - 财政年份:1999
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulatory mechanism of matrix metalloproteinase activity.
基质金属蛋白酶活性的调节机制。
- 批准号:
04680169 - 财政年份:1992
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Structures and functions of growth inhibitors secreted from rat liver cell lines
大鼠肝细胞系分泌的生长抑制剂的结构和功能
- 批准号:
63580123 - 财政年份:1988
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Properties and Physiological Roles of Cell Growth Inhibitors Present in Blood
血液中细胞生长抑制剂的特性和生理作用
- 批准号:
60480500 - 财政年份:1985
- 资助金额:
$ 8.96万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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