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Study on the Regulation of Thymic Hormone Secretion by Neural and Endocrine System-Using Primary Culture System of the Thymic Hormone-Secreting (Thymus Epthelial) Cells-

神经内分泌系统对胸腺激素分泌调节的研究-利用胸腺激素分泌（胸腺上皮）细胞原代培养系统-

基本信息

批准号：
63440018
负责人：
KINOSHITA Yoshihiro
金额：
$ 20.8万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1991
项目状态：
已结题

项目摘要

(1) Separation of biologically active polypeptides (BAPs) from the culture supernatant of thymus epithelial cells (TEC).We tried to separate BAPs from the culture supernatant of TEC line, IT-45R1. The polypeptide fraction with 2.5kD in molecular weisht possessed the capacity to induce the responsiveness of rat thymic lymphocytes (TLs) against concanavalin A and phytohemagglutinin. By further separation using reversed-phase column, the former was found in the hydrophobic fraction and its target cells were enriched in lighter TLs, while the latter was detected in the hydrophilic fraction and its target was heavier TLs. About 80% of tat TLs bind to guinea pig erythrocytes and from rosettes (RFC), whereas other TLs (non-RFC) do not. The 3kD fraction possessed the capacity to endow non-RFC with resette forming activity. Furthermore, rosette formation-inducing activity was found in the two different fractions separated by reversed-phase column method. By the same methods described above, the … More activity to induce proliferation of TLs was recognized in 10kD fraction from the culture supernatant of TAD3, derived from lymphocyte dominant thymoma. It is assumed that the target cells for 10kD fraction bear CD4-8- phenotype, observed in the most immature TLs and that the adherent cells in thymus take, also, an role in the proliferation system.(2) Effects of neurotransmitter and hormone on the function of thymus epithelial cells.The effect of cholinergic agonists on TEC was examined by using TAD3. It is revealed that cholinergic agonists stimulate TEC to proliferate at preconfluent state. On the other hand, at confluent state, the agonists stimulate TEC to synthesize proteins. These activities were possibly induced by the signal through nicotinic acetylcholine receptor on TEC surface. Furthermore, the effect of growth hormone on TEC was examined. It is suggested that the hormone enhances the mitotic index of the TAD3 cells at preconfluent state and enhances the protein synthesis of IT-45R1 cells at confluent. Furthermore, growth hormone is detected to increase thymosin alpha 1 aynthesis of IT-45R1 cells at confluent state by using anti-synthetic thymosin alpha 1 antibody, prepared by us. Less

(1)从胸腺上皮细胞（TEC）培养上清中分离生物活性多肽（BAPs），我们尝试从TEC株IT-45 R1的培养上清中分离BAPs。分子量为2.5kD的多肽组分具有诱导大鼠胸腺淋巴细胞对刀豆球蛋白A和植物血凝素的反应性。通过反相柱进一步分离，发现前者存在于疏水性组分中，其靶细胞富集较轻的TLs，而后者存在于亲水性组分中，其靶细胞富集较重的TLs。约80%达特TL与豚鼠红细胞结合，并且来自红细胞（RFC），而其他TL（非RFC）不结合。3 kD组分具有赋予非RFC以重置形成活性的能力。反相柱层析法分离得到的两个不同组分均具有诱导玫瑰花结的活性。通过上述相同的方法， ...更多信息 TAD 3的培养上清中10 kD组分具有诱导T淋巴细胞增殖的活性，TAD 3来源于淋巴细胞显性胸腺瘤。假设10 kD组分的靶细胞具有在最不成熟的TL中观察到的CD 4 -8-表型，并且胸腺中的贴壁细胞也在增殖系统中发挥作用。(2)神经递质和激素对胸腺上皮细胞功能的影响：采用TAD 3. 0软件检测胆碱能激动剂对TEC的影响。结果表明，胆碱能激动剂刺激TEC在融合前增殖。另一方面，在汇合状态下，激动剂刺激TEC合成蛋白质。这些活性可能是由TEC表面烟碱型乙酰胆碱受体介导的。此外，生长激素对TEC的影响进行了检查。提示该激素可提高TAD 3细胞融合前的有丝分裂指数，并促进IT-45 R1细胞融合时的蛋白质合成。此外，用我们制备的抗合成胸腺素α_1抗体检测到生长激素可增加融合状态的IT-45 R_1细胞的胸腺素α_1合成。少