Molecular Mechanism of Photoinhibition and Its Recovery of Photosynthetic Activity

光抑制的分子机制及其光合活性的恢复

• 批准号: 01304003
• 负责人: OKAYAMA Shigeki
• 金额: $ 7.04万
• 依托单位: Biological Laboratory, College of General Education, Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01304003/
• 关键词: Photosynthesis; Photoinhibition; Photosystem; Photoregulation; Chlorophyll; Reaction Center; D1 protein; Peroxidase; 光損傷; ペルオキシダ-ゼ; 光化学系; 反応中心; チトクロ-ム; アスコルビン酸ペルオキシダ-ゼ

Chlorophyll was found to be degraded enzymatically in the order of chlorophyride, pheophorbide and piropheophorbide. Epimerization of chlorophyll was also found to be selfcatalyzed by a central magnesium.Photoinhibition of the accumulation of photosynthetic pigments during the aerobic growth of a photosynthetic bacterium was shown to be regulated by photoregulation of translation of the apoprotein-gene.Photosystem I was found to have the cylclic electron transport system to avoid the photoinhibition by reducing the production of harmful active oxygens.Unsaturated fatty acids was found to be effective to avoid the photoinhibition of the photosystem II.The processing enzyme of photosystem II reaction center D1 protein was purified and characterized from the thylakoids.Photoregulation of the stoichiometry of thylakod components was shown to be effected by synthesis and supply of the chlorphyll a.Enzymatic and molecular propeties of peroxidases which scavenge active oxygens were studied.

叶绿素被发现在叶绿素，脱镁叶绿酸和焦脱镁叶绿酸的顺序酶降解。叶绿素的差向异构化也被发现是由一个中心的镁自催化的。光合细菌有氧生长过程中光合色素积累的光抑制被证明是由光调节脱辅基蛋白的翻译来调节的。光系统I具有环状电子传递系统，通过减少有害活性氧的产生来避免光抑制。从类囊体中纯化并鉴定了光系统II反应中心D1蛋白的加工酶，研究了叶绿素a的合成和供应对类囊体组分化学计量的光调节作用，并对分解活性氧的过氧化物酶的酶学和分子生物学性质进行了研究。

项目成果

Wise,R.R.,Terashima,I.and Ort,D.R.: "The effect of chilling in the light on photosphorylation" Photosynthesis Resarch. 25. 137-139 (1990)

Wise,R.R.、Terashima,I. 和 Ort,D.R.：“光中冷却对光磷酸化的影响”光合作用研究。

Kanematsu, S. and Asada, K.: "CuZn-Superoxide dismutases in rice : Occurrence of an active, monomeric enzyme and two isozymes in leaf and non-photosynthetic tissues" Plant Cell Physiol.30. 381-391 (1989)

Kanematsu, S. 和 Asada, K.：“水稻中的铜锌超氧化物歧化酶：叶和非光合组织中活性单体酶和两种同工酶的出现”Plant Cell Physiol.30。

Asada, K., Neubauer, C. Heber, U. and Shreiber, U.: "Methyl viologen-dependent cyclic electron transport in spinach chloroplasts in the absence of oxygen" Plant Cell Physiol.31. 557-564 (1990)

Asada, K.、Neubauer, C. Heber, U. 和 Shreiber, U.：“缺氧情况下菠菜叶绿体中甲基紫精依赖性循环电子传递”Plant Cell Physiol.31。

Wise, R. R., Terashima, I. and Ort, D. R.: "The effect of chilling in the light on photophosphorylation" Photosynthesis Reserch. 25. 137-139 (1990)

Wise, R. R.、Terashima, I. 和 Ort, D. R.：“光中冷却对光磷酸化的影响”光合作用研究。

Takahama, U. and Egashira, T.: "Peroxidases in vacuoles of Vicia faba leves" Phytochemistry. 30. 73-77 (1991)

Takahama, U. 和 Egashira, T.：“蚕豆液泡中的过氧化物酶”植物化学。