Studies on prenylquinones in green leaves
绿叶中异戊二烯醌的研究
基本信息
- 批准号:60540436
- 负责人:
- 金额:$ 1.28万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1985
- 资助国家:日本
- 起止时间:1985 至 1986
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Distributions and redox levels of prenylquinones in leaves and chloroplasts were studied using the methods developed here. The method is consisted of rapid extraction of the quinones on the sample preparation cartridge, followed by the separation and detection with the reversed-phase high-performance liquid chromatography using the dual-electrodes voltanmetric detector.1. More than half of plastoquinones in thylakoid membranes were found in lipid matrix, suggesting its role as mobile electron and proton carrier. The rest of plastoquinones were in particles as fixed electron/proton carriers. The molar ratios of each derivatives to chlorophyll were almost the same in every part of thylakoid membrane. Phylloquinone was concentrated in photosytem I particle, possibly as a redox component. The ratio of tocopherolquinone to chlorophyll was almost constant in every portion of thylakoid membrane, suggesting its role as a protectant for the photoinjury of pigment systems by strong light.2. During the development of spinach leaves the ratio of plastoquinone-C to chlorophyll was increased, and of ubiquinone-9 was decreased. The ratios of the other prenylquinones were not changed. It seems that the role of the respiratory system as ATP producing system is partly replaced by the photosynthetic system in the course of leaf development. Plasstoquinone C might be produced by photochemical epoxydation of plastoquinone A.3. The primary photochemical reaction in Photosystem II required two plastoquinone A molecules.4. The redox potential of plastoquinone A in chloroplasts was +20 mV at pH 7.8.
利用所建立的方法研究了异戊二烯醌在叶片和叶绿体中的分布和氧化还原水平。该方法采用样品预处理柱快速提取,反相高效液相色谱双电极伏安检测器分离检测.类囊体膜上的质体醌有一半以上存在于脂质基质中,表明它是移动的电子和质子的载体。其余的质体醌在颗粒中作为固定的电子/质子载体。在类囊体膜的各个部位,各衍生物与叶绿素的摩尔比基本相同。叶绿醌可能作为氧化还原组分富集在光系统I颗粒中。类囊体膜各部分中生育酚醌与叶绿素的比值几乎不变,表明其对色素系统的光损伤具有保护作用.在菠菜叶片发育过程中,质体醌-C与叶绿素的比值增加,而泛醌-9的比值降低。其他异戊二烯基醌的比例未发生变化。看来,在叶片发育过程中,呼吸系统作为ATP产生系统的作用部分被光合系统所取代。质体醌C可能通过质体醌A的光化学环氧化产生。光系统II的初级光化学反应需要两个质体醌A分子.叶绿体中质体醌A的氧化还原电位在pH 7.8时为+20 mV。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Tabata,S.Itoh,Y.Yamamoto,S.Okayama and M.Nishimura: "Two plastoquinone A molecules are required for photosystem II Activity: Analysis in hexane-extracted Photosystem II particles" Plant and Cell Physiology. 26. 858-863 (1985)
K.Tabata、S.Itoh、Y.Yamamoto、S.Okayama 和 M.Nishimura:“光系统 II 活性需要两个质体醌 A 分子:己烷提取的光系统 II 颗粒中的分析”植物和细胞生理学。
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- 影响因子:0
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- 通讯作者:
Y.Isogai, M.Nishimura and S.Okayama: "Simultaneous determination of plastoquinone A and plastoquinol A by high-performance liquid chromatography with an electrochemical detector; A rapid and simple procedure for determination of the redox level of" plasto
Y.Isogai、M.Nishimura 和 S.Okayama:“用电化学检测器通过高效液相色谱同时测定质体醌 A 和质体醌醇 A;快速、简单地测定质体醌 A 和质体醌醇 A 的氧化还原水平”
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- 影响因子:0
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Shigeki Okayama: "High-performance liquid chromatography of prenylquinones in green leaves using an electrochemical detector" Report of the center of advanced instrumental analysis, Kyushu University. 4. 51-55 (1986)
冈山茂树:“使用电化学检测器对绿叶中异戊二烯醌进行高效液相色谱分析”九州大学先进仪器分析中心的报告。
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- 影响因子:0
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K.Tabata;S,Itoh;Y.Yamamot;S.Okayama;M.Nishimura: Plant and Cell Physiology. 26. 855-863 (1985)
K.Tabata;S,Itoh;Y.Yamamot;S.Okayama;M.Nishimura:植物和细胞生理学。
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OKAYAMA Shigeki其他文献
OKAYAMA Shigeki的其他文献
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{{ truncateString('OKAYAMA Shigeki', 18)}}的其他基金
May Cytochrome b-559 function to protect photosystem II from ligh stress?
细胞色素 b-559 可以发挥保护光系统 II 免受轻应激的作用吗?
- 批准号:
06640845 - 财政年份:1994
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Molecular Mechanism of Photoinhibition and Its Recovery of Photosynthetic Activity
光抑制的分子机制及其光合活性的恢复
- 批准号:
01304003 - 财政年份:1989
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
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