Studies on production of new stock (Matthiola incana R.Br) varieties with dark yellow-colored flowers.

本试验通过砧木与背花（桂竹香）杂交，培育出深黄色花的砧木新品种，1.砧木与壁花的杂交：通过两种植物杂交获得的植株，多数性状和开花早性与杂交母本相似。通过杂交M.ineana cv. 'Shiho'x C.cheiri cv.“Aliony橙子”与母本的紫色不同，也与M.incana cv.“Sosetsu' C.cheiri cy. 2.砧木愈伤组织原生质体的分离与培养：原生质体的产量与母本不同，母本为白色，母本为白色。 ...更多信息 在光照条件下，砧木愈伤组织的诱导率高于黑暗条件。在黑暗条件下，愈伤组织原种的原生质体仍能继续分裂并形成菌落，在MS改良培养基中加入0.3mg/l BA培养3个月后，愈伤组织仍能形成。3.原生质体的电融合：原生质体珠链形成和原生质体融合的最佳条件分别为：原生质体密度为105 /ml，高频电场为150 V/cm，交变频率为1 MHz，持续20秒，4.重瓣花砧木的组织培养快繁：叶片、下胚轴和下胚轴愈伤组织在1/2 MS改良培养基上培养，附加BA或玉米素（1 mg/l），L-谷氨酸（30 mm），以补充NH_4NO_3和KNO_3作为氮源，可作为离体芽形成的主要外植体。少

The following experiments were carried out to produce new varieties of stock(Hatthiola incana R.Br) with dark yellow-colored flowers through crossing between stock and-wall flower(Cheiranthus cheiri L.), fertilized ovule cultuie, in vitro fertilization, and protoplast fusion.The micropropagation was also applied to obtain a number of the stock plants which were useful for commercial markets.1.Crossing between stock and wall flower: The characters and flowering earliness of most of the plants, which were obtained from crossing between the above two plants, were similar to those of the female parents in crossing. The flower color(white) of the plants, obtained through the cross M.ineana cv.'Shiho'x C.cheiri cv.'Aliony orange' was different from that (violet) of the female parent, as well as that (pale yellow) of M.incana cv.'Sosetsu' C.cheiri cy.'all double' was different from that(white) of the female parent.2.Protoplast isolation and culture from stock callus: The yield of protoplast f … More rom stock callus under the light condition was higher than that under the dark condition. However, prtoplasts from stock callus under dark condition continued to divide and grew into colonies, and calluses on the MS modified medium with 0.3 mg/l BA after 3 months of incubation.3.Electrofusion of Protoplasts: The optimal conditions for the formation of pearl chains of protoplasts and the fusin of protoplasts were as follows respectively : a protoplast density of 105 /ml and a high frequency electric field of 150 v/cm under a alternative frequency of 1 MHz for 20 sec., and a pulse field strength of 1.5 Kv/ cm and a pulse width of 70 mu s.4.Micropropagation of stock with double flowers through tissue culture :Leaf and hypocotyl sections and hypocotyl-derived calluses could be applied as prominent explants for in vitro shoot formation when cultured on 1/2MS modified medium supplemented with BA or zeatin (i mg/1), and L-glutamate (30 mm) in compensation for NH_4NO_3 and kNO_3 as a nitrogen source. Less