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Alteration in the secretory function of adrenal medulla and its relation to the condition of a disease in diabetes.

肾上腺髓质分泌功能的改变及其与糖尿病病情的关系。

基本信息

批准号：
63570129
负责人：
MORITA Kyoji
金额：
$ 1.34万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Hypertension and congestive heart-failure are often observed in patients with diabetes, and these complications are thought to be closely related to an alteration in the functions of sympathoadrenergic systems, which may be attributed to the long-lasting exposure to the high concentration of blood sugar. However, there has been no conclusive evidence supporting this possibility. Then, to address the question of whether the sympathoadrenergic functions are influenced by the exposure to high concentration of glucose, chromaffin cells isolated from bovine adrenal medulla were cultured in the medium containing high concentration of glucose (7-21 days), and the amount of catecholamines within these cells was then determined. In consequence, catecholamine content in the cells maintained in the normal culture medium was markedly decreasing as a function of the culture days. In contrast, the decrease in catecholamine content was no more observed when the cells were maintained in the high-gluco … More se medium. However, the same concentrations of other sugars, such as sucrose and raffinose, failed to prevent a decline in the cellular content of catecholamines under the same culture conditions.It therefore seems conceivable that the turnover of catecholamines in the adrenal chromaffin cell is thought to be directly affected by the exposure to high concentration of glucose. On the other hand, we have examined the effects of hypoglycemic sulfonylureas on the secretion and the biosynthesis of catecholamines in cultured chromaffin cells, and found that these drugs inhibited both the secretion and the biosynthesis. Furthermore, sulfonylureas were also shown to inhibit the calcium uptake into the cells. Since the biosynthesis as well as the secretion of catecholamines is well known to require the influx of calcium ions into the cells, it therefore seems likely that sulfonylureas inhibits both catecholamine secretion and biosynthesis in adrenal chromaffin cells, probably through their inhibitory actions on the calcium influx into the cells. Less

高血压和充血性心力衰竭经常在糖尿病患者中观察到，这些并发症被认为与交感肾上腺素能系统功能的改变密切相关，这可能归因于长期暴露于高浓度血糖。然而，一直没有确凿的证据支持这种可能性。然后，为了解决交感肾上腺素能功能是否受到暴露于高浓度葡萄糖的影响的问题，将从牛肾上腺髓质分离的嗜铬细胞培养在含有高浓度葡萄糖的培养基中（7-21天），然后测定这些细胞内的儿茶酚胺的量。结果，在正常培养基中维持的细胞中的儿茶酚胺含量作为培养天数的函数显著降低。相反，当细胞维持在高葡萄糖浓度时，不再观察到儿茶酚胺含量的降低。 ...更多信息中等。然而，相同浓度的其他糖，如蔗糖和棉子糖，未能阻止在相同的培养条件下，细胞中的儿茶酚胺含量的下降。因此，似乎可以想象，在肾上腺嗜铬细胞中的儿茶酚胺周转被认为是直接影响暴露于高浓度的葡萄糖。另一方面，我们研究了降糖磺脲类药物对培养的嗜铬细胞分泌和生物合成儿茶酚胺的影响，发现这些药物抑制分泌和生物合成。此外，磺酰脲类药物也显示出抑制钙摄取到细胞中。众所周知，由于儿茶酚胺的生物合成和分泌需要钙离子流入细胞，因此磺酰脲类药物可能通过抑制钙离子流入细胞而抑制肾上腺嗜铬细胞中的儿茶酚胺分泌和生物合成。少