Histaminergic regulation of the neuroendocrine system: An analysis using microdialysis of the hypothalamus of conscious and freely moving rats.
神经内分泌系统的组胺能调节:使用微透析对有意识和自由活动的大鼠的下丘脑进行分析。
基本信息
- 批准号:63570128
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Recently, the histaminergic neurons have been identified in mammalian brains by immunocytochemical methods. The cell bodies of the histaminergic neurons are localized in the tuberomammillary nucleus in the posterior hypothalamic region, while the fibers are found in almost all regions of the brain. Especially in the hypothalamic nuclei, the highest density of the histaminergic fibers were found, suggesting the involvement of the central histaminergic system in the regulation of neuroendocrine system, However, detailed neurochemical mechanism of the histaminergic neurotransmission and its physiological roles are still not clear.Among the brain functions, the hypothalamo-hypophyseal neuroendocrine system is the only one whose function can be determined quantitatively by measurement of the plasma levels of the hormones. In this study, we applied the microdialysis technique to determine the dynamics of the neuronal histamine in the hypothalamus, and we tried to correlate the histamine rele … More ase in the hypothalamus and the levels of several hormones in blood to elucidate the physiological roles of histamine in the neuroendocrine system.By improving the dialysis conditions and the assay sensitivity, we could detect histamine release from anterior hypothalamic area. By several lines of neuropharmacological studies, we could also confirm that histamine measured by the microdialysis was released from the nerve terminals. In the freely moving rats, the histamine release was found to show a clear circadian variation, decreasing during the mid-light period, then rapidly increasing and keeping high levels during the dark period. In another experiment, circadian variation of the plasma levels of corticosterone and ACTH was disappeared by chronic administration of the specific inhibitor of histamine synthesis. In conclusion, the release of endogenous neuronal histamine in rat brain was successfully monitored using a new technique of microdialysis, and this technique will be useful to investigate the histaminergic regulation of the neuroendocrine system. Less
近年来,利用免疫细胞化学方法在哺乳动物大脑中发现了组胺能神经元。组胺能神经元的细胞体定位于下丘脑后区结节乳头核,而其纤维几乎分布于大脑的所有区域。特别是在下丘脑核中,组胺能纤维密度最高,提示中枢组胺能系统参与神经内分泌系统的调节,但组胺能神经传递的详细神经化学机制及其生理作用尚不清楚。在脑功能中,下丘脑-垂体神经内分泌系统是唯一可以通过测量血浆激素水平来定量确定其功能的系统。在本研究中,我们应用微透析技术测定了下丘脑神经元组胺的动态变化,并试图将下丘脑的组胺释放与血液中几种激素的水平联系起来,以阐明组胺在神经内分泌系统中的生理作用。通过改善透析条件和检测灵敏度,我们可以检测下丘脑前部区组胺释放。通过几条神经药理学研究,我们也可以证实微透析测量的组胺是从神经末梢释放出来的。自由运动大鼠组胺释放具有明显的昼夜变化规律,在光照中期下降,在黑暗时期迅速上升并保持较高水平。在另一项实验中,长期服用特定的组胺合成抑制剂,血浆皮质酮和ACTH水平的昼夜变化消失。综上所述,利用微透析技术成功地监测了大鼠脑内源性神经元组胺的释放,该技术将为研究神经内分泌系统的组胺能调控提供依据。少
项目成果
期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
S.Ohtsuka: "Estrogen stimulates gonadotropin-releasing hormone release from rat hypothalamus independently through catecholamine and hitamine in vitro." Acta Endocrinol(Copenh). 120. 644-648 (1989)
S.Ohtsuka:“雌激素在体外通过儿茶酚胺和hitamine独立刺激大鼠下丘脑促性腺激素释放激素的释放。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Yamatodani, T.Mochizuki, K.Okakura & H.Wada: "An in vivo study histamine release in rat hypothalamus using intracerebral microdialysis." Agents and Actions (1990).
A.山谷、T.望月、K.冈仓
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
N.Inagaki: "An analysis of histaminergic efferents of the thuberomammillary nucleus to the medial preoptic area and inferior colliculus of the rat." Exp.Brain Res.(1990)
N.Inagaki:“对大鼠内侧视前区和下丘的Thuberomammillary 核的组胺能传出信号的分析。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
R.Cacabelos: "Antagonistic effects of growth hormone-releasing factor and somatostatin on brain histamine." Endocrinology. 122. 1269-1276 (1988)
R.Cacabelos:“生长激素释放因子和生长抑素对脑组织胺的拮抗作用。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Yamatodani: "An in vivo study histamine release in rat hypothalamus using intracerbral microdialysis." Agents and Actions. (1990)
A.Yamatodani:“使用脑内微透析对大鼠下丘脑组胺释放进行体内研究。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YAMATODANI Atsushi其他文献
YAMATODANI Atsushi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YAMATODANI Atsushi', 18)}}的其他基金
The role of histamine H4 receptor in the development of cancer-related fatigue
组胺 H4 受体在癌症相关性疲劳发生中的作用
- 批准号:
21590279 - 财政年份:2009
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of selective ligands to histamine H3 and H4 receptors and its application to elucidate the physiological function of the receptors.
组胺 H3 和 H4 受体选择性配体的开发及其在阐明受体生理功能中的应用。
- 批准号:
15590225 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Pathophysiological significance of inducible histamine in the brain.
大脑中诱导型组胺的病理生理学意义。
- 批准号:
10670141 - 财政年份:1998
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of the in vivo monitoring system of nitric oxide using a micro electrode.
使用微电极开发一氧化氮体内监测系统。
- 批准号:
07557019 - 财政年份:1995
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Physiological significance of histamine H3-receptor as hetero-recetor in the brain.
组胺 H3 受体作为大脑异质受体的生理意义。
- 批准号:
07680843 - 财政年份:1995
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cell Biological Compartmentation of Brain Histamine
脑组胺的细胞生物区室
- 批准号:
05670090 - 财政年份:1993
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Physiological significance of the histaminergic system : A brain microdialysis study using unanesthetized and freely-moving rats
组胺能系统的生理意义:使用未麻醉且自由活动的大鼠进行脑微透析研究
- 批准号:
02670085 - 财政年份:1990
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
NEUROENDOCRINOLOGICAL ROLES OF HISTAMINE IN THE HYPOTHALAMO-HYPOPHYSEAL SYSTEM
组胺在下丘脑-垂体系统中的神经内分泌作用
- 批准号:
60480135 - 财政年份:1985
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
An HPLC method for determination of dopamine, dopamine-3-O- and dopamine-4-O-sulfate in plasma, urine and tissues.
高效液相色谱法测定血浆、尿液和组织中的多巴胺、3-O-多巴胺和4-O-硫酸多巴胺。
- 批准号:
60870015 - 财政年份:1985
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
相似海外基金
In Vivo Brain Dialysis of Neuropeptides and Neuroimmune Signaling Proteins
神经肽和神经免疫信号蛋白的体内脑透析
- 批准号:
8244124 - 财政年份:2011
- 资助金额:
$ 1.41万 - 项目类别:
In Vivo Brain Dialysis of Neuropeptides and Neuroimmune Signaling Proteins
神经肽和神经免疫信号蛋白的体内脑透析
- 批准号:
8328931 - 财政年份:2011
- 资助金额:
$ 1.41万 - 项目类别: