BRAIN DIALYSIS AND COCAINE SELF-ADMINISTRATION

该提案要求提供必要的设备，以扩大初步研究 脑内透析与行为测量相结合 在静脉注射可卡因期间移动大鼠。与 用于体内微透析的终端设备， 将建立微量取样系统， 多巴胺及其代谢物的突触浓度的取样 在3小时的自我管理会议期间。施行本 实验将研究自我服用可卡因对 多巴胺在几个终端区域的释放， 可卡因奖励和依赖性和精神病理学的发展 与可卡因滥用有关。多巴胺的突触水平 每隔5分钟在脑桥核、前额叶 皮质和纹状体，并与动物的模式相比， 对药物的反应。还将尝试调查 与急性相比， 可卡因自我管理这些研究具有重要意义 对精神兴奋剂奖励中关键回路的理解 和依赖。

This proposal requests equipment necessary to extend preliminary studies combining intracerebral dialysis with behavioral measurement in freely moving rats during intravenous self-administration of cocaine. To that end facilities for in vivo microdialysis supported by an appropriate microsampling system will be established that will permit frequent sampling of synaptic concentrations of dopamine and its metabolites during 3 hour self-administration sessions. the purpose of these experiments will be to study the effects of self-administered cocaine on dopamine release in several terminal areas that have been implicated in cocaine reward and in the development of dependence and psychopathology associated with cocaine abuse. Synaptic levels of dopamine will be monitored at 5 minute intervals in the nucleus accumbens, prefrontal cortex and corpus striatum and compared to the animal's pattern of responding for the drug. Attempts will also be made to investigate specific changes in dopamine function after repeated as opposed to acute cocaine self administration. These studies have important implications for an understanding of critical circuitries in psychostimulant reward and dependence.