Mechanisms of cell proliferation and extracellular matrix production in glomeruli

在上述资助的帮助下，我们对肾小球细胞增殖和细胞外基质增加的机制进行了研究和报道。研究期间取得了以下结果：1)人(肾活检)和实验动物炎症肾小球内单核巨噬细胞系细胞的电子显微镜和免疫组织化学检测；2)尿蛋白的电泳谱与肾小球肾炎(GN)模型的形态表达差异相对应。3)利用分离的肾小球培养，我们在体外发现了一种能够诱导肾小球系膜细胞增殖的人单核巨噬细胞来源的因子。4)此外，我们在人单核细胞培养上清液中检测到细胞外基质产生的增强活性。5)我们报道了一种新的实验性肾炎模型，在该模型中，肾小球发生明显的渗出性和增殖性变化，细胞内含有Ia阳性的白细胞。利用这种半抗原特异性肾小球损伤模型，可以在分子和细胞水平上研究诱导肾小球内细胞浸润和细胞增殖的机制。

With aid of the above grant, we have researched and reported on the mechanisms of cell proliferation and extracellular matrix increment in the renal glomeruli. The following results have been obtained during the period of research project:1).Monocyte-macrophage line cells were detected electron microscopically and immunohistologically within the inflamed glomeruli from human (using renal biopsy) and experimental animals.2).Electrophoretic patterns of urinary proteins corresponded to differences in morphological expression such as proliferative changes in experimental glomerulonephritis (GN) models.3).Using culture of isolated glomeruli, we found a human monocute-derived factors which were able to induce proliferation of glomerular mesangial cells in vitro. One of this factors is similar in nature to platelet- derived growth factor.4).Furthermore, we detected the enhancing activity of extracelluar matrix production in the culture supernatant of human monocytes.5).We reported a new model of experimental GN model in which marked exudative and proliferative changes in glomeruli and cellular influx containing Ia-positive leukocytes. Using this model of hapten-specific glomerular injury, mechanisms responsible for inducing cell infiltration and cell proliferation within glomeruli could be researched at molecular and cellular levels.