Mechanisms of cell proliferation and extracellular matrix production in glomeruli
Mechanisms of cell proliferation and extracellular matrix production in glomeruli
批准号:
63570155
负责人:
OITE Takashi
金额:
$1.41万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1988
资助国家:
日本
项目状态:
已结题
起止时间:
1988 至 1989
中文摘要
在上述资助的帮助下,我们对肾小球细胞增殖和细胞外基质增加的机制进行了研究和报道。研究期间取得了以下结果:1)人(肾活检)和实验动物炎症肾小球内单核巨噬细胞系细胞的电子显微镜和免疫组织化学检测;2)尿蛋白的电泳谱与肾小球肾炎(GN)模型的形态表达差异相对应。3)利用分离的肾小球培养,我们在体外发现了一种能够诱导肾小球系膜细胞增殖的人单核巨噬细胞来源的因子。4)此外,我们在人单核细胞培养上清液中检测到细胞外基质产生的增强活性。5)我们报道了一种新的实验性肾炎模型,在该模型中,肾小球发生明显的渗出性和增殖性变化,细胞内含有Ia阳性的白细胞。利用这种半抗原特异性肾小球损伤模型,可以在分子和细胞水平上研究诱导肾小球内细胞浸润和细胞增殖的机制。
英文摘要
With aid of the above grant, we have researched and reported on the mechanisms of cell proliferation and extracellular matrix increment in the renal glomeruli. The following results have been obtained during the period of research project:1).Monocyte-macrophage line cells were detected electron microscopically and immunohistologically within the inflamed glomeruli from human (using renal biopsy) and experimental animals.2).Electrophoretic patterns of urinary proteins corresponded to differences in morphological expression such as proliferative changes in experimental glomerulonephritis (GN) models.3).Using culture of isolated glomeruli, we found a human monocute-derived factors which were able to induce proliferation of glomerular mesangial cells in vitro. One of this factors is similar in nature to platelet- derived growth factor.4).Furthermore, we detected the enhancing activity of extracelluar matrix production in the culture supernatant of human monocytes.5).We reported a new model of experimental GN model in which marked exudative and proliferative changes in glomeruli and cellular influx containing Ia-positive leukocytes. Using this model of hapten-specific glomerular injury, mechanisms responsible for inducing cell infiltration and cell proliferation within glomeruli could be researched at molecular and cellular levels.
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Morioka T et al.: "The electrophoretic pattern of urinary protein in situ immune complex glomerulonephritis." Nephron 50:116-120(1988).
Morioka T 等人:“尿蛋白原位免疫复合物肾小球肾炎的电泳模式。”
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Nakamura T et al.: "Monoclonal antibodies to human glomerular antigens." Virchows Archiv A Pathol Anat Histopathol. 412:573-582.
Nakamura T 等人:“针对人肾小球抗原的单克隆抗体。”
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Kagami S et al.: "Active in situ immune complex glomerulonephritis using the hapten-carrier system: role of epitope density in cationic antigens." Clin exp Immunol 74:121-125(1988).
Kagami S 等人:“使用半抗原载体系统的活性原位免疫复合物肾小球肾炎:表位密度在阳离子抗原中的作用。”
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Oite T et al.: "Glomerular deposition of immune complexes and the role of infiltrating monocytes." "Recent Studies of IgA Nephropathy in Japan", edited by M. Arakawa and F. Gejyo, Nishimura Co Ltd, Niigata, p 3-22(1989).
Oite T 等人:“免疫复合物的肾小球沉积和浸润单核细胞的作用。”
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Oite,Takashi: "Hapten-specific cellular immune response producing glomerular injury." Clin exp Immounol. 76. 463-468 (1989)
Oite,Takashi:“半抗原特异性细胞免疫反应产生肾小球损伤。”
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共 24 条
Functional analysis of mesangial cell syncytium and elucidation of mechanisms involving progressive glomerulosclerosis
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批准号:15390266
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.42万
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财政年份:2003
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负责人:OITE Takashi
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依托单位:
Identification of the molecular epitope associated with mesangical cell function.
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批准号:08670241
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.47万
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财政年份:1996
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负责人:OITE Takashi
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依托单位:
Regulation loss of mesangial cell function leading to irreversible, glomerular sclerosis.
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批准号:06670215
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1994
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负责人:OITE Takashi
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依托单位:
Mechanisms of sclerosis in the glomerulus as a representative of microcirculation
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批准号:04670199
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$0.26万
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财政年份:1992
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负责人:OITE Takashi
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依托单位:
In situ immune complex nephritis using cationic antigens - mediator system involved in the glomerular injury -
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批准号:60570153
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1985
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负责人:OITE Takashi
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依托单位:
海外基金