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Mechanisms of cell proliferation and extracellular matrix production in glomeruli

Mechanisms of cell proliferation and extracellular matrix production in glomeruli
肾小球细胞增殖和细胞外基质产生的机制
批准号:
63570155
负责人:
OITE Takashi
金额:
$1.41万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1988
资助国家:
日本
项目状态:
已结题
起止时间:
1988 至 1989

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项目成果

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中文摘要
翻译
在上述资助下,我们研究并报道了肾小球细胞增殖和细胞外基质增加的机制。在研究项目期间,获得了以下结果:1)。在人(肾活检)和实验动物的炎症肾小球内用电镜和免疫组织学方法检测单核-巨噬细胞系细胞。在实验性肾小球肾炎(GN)模型中,尿蛋白的电泳模式对应于形态学表达的差异,如增生性变化。通过离体肾小球的培养,我们发现了一种能够诱导肾小球系膜细胞体外增殖的人单源性因子。其中一个因子在性质上类似于血小板衍生的生长因子。此外,我们在人单核细胞培养上清液中检测到细胞外基质生产的增强活性。我们报道了一种新的实验性GN模型,其中肾小球和含有ia阳性白细胞的细胞内流有明显的渗出和增殖变化。利用这种半抗原特异性肾小球损伤模型,可以在分子和细胞水平上研究肾小球内诱导细胞浸润和细胞增殖的机制。
英文摘要
With aid of the above grant, we have researched and reported on the mechanisms of cell proliferation and extracellular matrix increment in the renal glomeruli. The following results have been obtained during the period of research project:1).Monocyte-macrophage line cells were detected electron microscopically and immunohistologically within the inflamed glomeruli from human (using renal biopsy) and experimental animals.2).Electrophoretic patterns of urinary proteins corresponded to differences in morphological expression such as proliferative changes in experimental glomerulonephritis (GN) models.3).Using culture of isolated glomeruli, we found a human monocute-derived factors which were able to induce proliferation of glomerular mesangial cells in vitro. One of this factors is similar in nature to platelet- derived growth factor.4).Furthermore, we detected the enhancing activity of extracelluar matrix production in the culture supernatant of human monocytes.5).We reported a new model of experimental GN model in which marked exudative and proliferative changes in glomeruli and cellular influx containing Ia-positive leukocytes. Using this model of hapten-specific glomerular injury, mechanisms responsible for inducing cell infiltration and cell proliferation within glomeruli could be researched at molecular and cellular levels.
期刊论文(26)
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会议论文
Morioka T et al.: "The electrophoretic pattern of urinary protein in situ immune complex glomerulonephritis." Nephron 50:116-120(1988).
Morioka T 等人:“尿蛋白原位免疫复合物肾小球肾炎的电泳模式。”
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Kagami S et al.: "Active in situ immune complex glomerulonephritis using the hapten-carrier system: role of epitope density in cationic antigens." Clin exp Immunol 74:121-125(1988).
Kagami S 等人:“使用半抗原载体系统的活性原位免疫复合物肾小球肾炎:表位密度在阳离子抗原中的作用。”
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24
    Functional analysis of mesangial cell syncytium and elucidation of mechanisms involving progressive glomerulosclerosis
    • 批准号:
      15390266
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $7.42万
    • 财政年份:
      2003
    • 负责人:
      OITE Takashi
    • 依托单位:
    Identification of the molecular epitope associated with mesangical cell function.
    • 批准号:
      08670241
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.47万
    • 财政年份:
      1996
    • 负责人:
      OITE Takashi
    • 依托单位:
    Regulation loss of mesangial cell function leading to irreversible, glomerular sclerosis.
    • 批准号:
      06670215
    • 项目类别:
      Grant-in-Aid for General Scientific Research (C)
    • 资助金额:
      $1.34万
    • 财政年份:
      1994
    • 负责人:
      OITE Takashi
    • 依托单位:
    Mechanisms of sclerosis in the glomerulus as a representative of microcirculation
    • 批准号:
      04670199
    • 项目类别:
      Grant-in-Aid for General Scientific Research (C)
    • 资助金额:
      $0.26万
    • 财政年份:
      1992
    • 负责人:
      OITE Takashi
    • 依托单位:
    海外基金