Mechanisms of cell proliferation and extracellular matrix production in glomeruli

在上述资助下，我们研究并报道了肾小球细胞增殖和细胞外基质增加的机制。在研究项目期间，获得了以下结果：1)。在人（肾活检）和实验动物的炎症肾小球内用电镜和免疫组织学方法检测单核-巨噬细胞系细胞。在实验性肾小球肾炎（GN）模型中，尿蛋白的电泳模式对应于形态学表达的差异，如增生性变化。通过离体肾小球的培养，我们发现了一种能够诱导肾小球系膜细胞体外增殖的人单源性因子。其中一个因子在性质上类似于血小板衍生的生长因子。此外，我们在人单核细胞培养上清液中检测到细胞外基质生产的增强活性。我们报道了一种新的实验性GN模型，其中肾小球和含有ia阳性白细胞的细胞内流有明显的渗出和增殖变化。利用这种半抗原特异性肾小球损伤模型，可以在分子和细胞水平上研究肾小球内诱导细胞浸润和细胞增殖的机制。

With aid of the above grant, we have researched and reported on the mechanisms of cell proliferation and extracellular matrix increment in the renal glomeruli. The following results have been obtained during the period of research project:1).Monocyte-macrophage line cells were detected electron microscopically and immunohistologically within the inflamed glomeruli from human (using renal biopsy) and experimental animals.2).Electrophoretic patterns of urinary proteins corresponded to differences in morphological expression such as proliferative changes in experimental glomerulonephritis (GN) models.3).Using culture of isolated glomeruli, we found a human monocute-derived factors which were able to induce proliferation of glomerular mesangial cells in vitro. One of this factors is similar in nature to platelet- derived growth factor.4).Furthermore, we detected the enhancing activity of extracelluar matrix production in the culture supernatant of human monocytes.5).We reported a new model of experimental GN model in which marked exudative and proliferative changes in glomeruli and cellular influx containing Ia-positive leukocytes. Using this model of hapten-specific glomerular injury, mechanisms responsible for inducing cell infiltration and cell proliferation within glomeruli could be researched at molecular and cellular levels.