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Development of a dynamic analysis method for phagocytic activity utilizing flow cytometry

利用流式细胞术开发吞噬细胞活性动态分析方法

基本信息

批准号：
63570170
负责人：
FUJIKAWA Kohzaburo
金额：
$ 1.41万
依托单位：
Kanazawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570170/
关键词：
Flow cytometry Phagocytosis Microspheres フローサイトメトリー貧食作用培養細胞

项目摘要

A new method to analyze the phagoctic activity utilizing flow cytometry (FCM) was developed. We observed the changes in the fraction of cell phagocytizing microspheres and concluded that the phagocytosis of microspheres in V79 cultured cell line could be described with an experimental equation with two parameters. The results were shown in the following twelve sections. 1) Representative examples of FCM measurements of phagocytosis were shown. 2) Results form FCM and microscopic measurements were compared and the reliability of FCM measurements was confirmed. 3) How to distinguish phagocytized microspheres from attached those was explained. 4) Microsphere size suitable to the FCM measurements was examined. 5) Equilibrium of microspheres surrounding cells was examined. 6) Increase in coefficient of variation of peaks in fluorescence histograms was explained by the localization of microspheres in cells. 7) Phagocytic activity was compared between macrophages and V79 cells. 8) An experimental equation describing pliagocytosis was demonstrated. 9) Effects of microsphere concentration on the phagocytosis parameters k and n was examined. 10) A computer program to analyze the phagocytic activity was shown. 11) Cell cycle dependency of phagocytic activity was discussed. 12) Effects of hydroxyurea on the phagoeytosis were discussed.

建立了一种利用流式细胞术（FCM）分析噬菌体吞噬活性的新方法。结果表明，微球对V79细胞的吞噬作用可用双参数实验方程描述。结果显示在以下12个部分中。1)示出了吞噬作用的FCM测量的代表性实例。2)将流式细胞仪检测结果与显微镜检测结果进行了比较，证实了流式细胞仪检测结果的可靠性。3)说明了如何区分吞噬微球和附着微球。4)检查了适合于FCM测量的微球尺寸。5)检测微球在细胞周围的平衡。6)荧光直方图中峰的变异系数的增加由微球在细胞中的定位来解释。7)比较了巨噬细胞和V79细胞的吞噬活性。8)证明了一个描述噬菌体作用的实验方程。9)考察了微球浓度对吞噬参数k和n的影响。10)并编制了吞噬活性分析的计算机程序。11)对吞噬活性的细胞周期依赖性进行了讨论。12)并讨论了羟基脲对吞噬作用的影响。