Study for establishment of polyploidized culture cell lines and transformation through polyploidization

多倍化培养细胞系的建立及多倍化转化的研究

• 批准号: 13670228
• 负责人: FUJIKAWA Kohzaburo
• 金额: $ 2.3万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670228/
• 关键词: Meth-A cells; V79 cells; tetraploid cell line; triploid cell line; octaploid cell line; polyploidization; demecolcine; fractal structure; polyloidization; K-252a; demecolcine

This study aims to establish polyploid cells and to examine transformation in the cells. We have established polyploid Meth-A cell lines (4-, 6-, 8-, 10-, 12-, 16-and 18-ploid) and polyploid V79 cell lines (3-, 4-and 6-ploid). The transformation through polyploidization has been reported for the temperature dependence, serum concentration dependence and expression of cell-surface hydrocarbon chain.This study intends to change a concept that chromosomes are independent each other in cells. I have made a model (genome-fractal structure model) based on the model that Manabu Takahashi (emeritus professor of Yamaguchi University) has introduced, and explained the experimental results using the model (Cell Prolif. 2002, 2003).In order to enlighten about DNA structure in cells, I have published two books (the one is of translated book of Manabu Takahashi). A half of this RESEARCH RESULTS will be described for the genome-fractal structure model.

本研究的目的是建立多倍体细胞，并检查在细胞中的转化。我们建立了多倍体Meth-A细胞系（4-、6-、8-、10-、12-、16-和18-倍体）和多倍体V79细胞系（3-、4-和6-倍体）。通过多倍体化的转化已被报道为温度依赖性、血清浓度依赖性和细胞表面烃链的表达，本研究旨在改变细胞中染色体相互独立的观念。基于高桥学（山口大学名誉教授）介绍的模型，制作了模型（基因组分形结构模型），并使用该模型（Cell Prolif. 2002，2003）.为了启发细胞中的DNA结构，我出版了两本书（一本是高桥学的翻译书）。一半的研究结果将被描述为基因组分形结构模型。

项目成果

Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H: "Effect of hydrochloric acid on the proliferation of tetraploid Meth-A cells."Cytometry Res.. 13. 19-24 (2003)

Fujikawa-Yamamoto K、Miyagoshi M、Yamagishi H：“盐酸对四倍体 Meth-A 细胞增殖的影响。”细胞计数研究 13. 19-24 (2003)

Miyagoshi M, Fujikawa-Yamamoto K, Yamagishi H: "The growth of diploid and tetraploid Meth-A cells in co-cultures at low temperature."J. Kanazawa Med. Univ.. 28. 69-73 (2003)

Miyagoshi M、Fujikawa-Yamamoto K、Yamagishi H：“低温共培养中二倍体和四倍体 Meth-A 细胞的生长。”J。

Fujikawa-Yamamoto K, Yamagishi H, Miyagoshi M: "Establishment of a triploid V79 cell line from tetraploid cells obtained through polyploidization using K-252a"Cell Prolif.. 35. 369-379 (2002)

Fujikawa-Yamamoto K、Yamagishi H、Miyagoshi M：“利用 K-252a 通过多倍体化获得的四倍体细胞建立三倍体 V79 细胞系”Cell Prolif.. 35. 369-379 (2002)

Fujikawa-Yamamoto K, Sakuma M: "Maintenance of quantity and density of membrane glycochains in diploid-tetraploid and tetraploid-octaploid transitions, respectively of Meth-A cells demonstrated by lectin binding"Cytologia. 68. 191-198 (2003)

Fujikawa-Yamamoto K、Sakuma M：“通过凝集素结合证明 Meth-A 细胞的二倍体-四倍体和四倍体-八倍体转变中膜糖链数量和密度的维持”Cytologia。

佐久真正弘, 藤川孝三郎: "Meth-A細胞における倍数性の変化に伴うレクチン結合性の変化について"Cytometry Res.. 13. 39-44 (2003)

Masahiro Saku、Kozaburo Fujikawa：“Meth-A 细胞中倍性变化导致凝集素结合的变化” Cytometry Res.. 13. 39-44 (2003)