Study for establishment of polyploidized culture cell lines and transformation through polyploidization

本研究旨在建立多倍体细胞，并检测细胞内的转化情况。我们建立了多倍体Meth-A细胞系(4、6、8、10、12、16和18倍体)和多倍体V79细胞系(3、4和6倍体)。多倍化转化具有温度依赖性、血药浓度依赖性和细胞表面碳氢链表达的特点，本研究旨在改变细胞中染色体相互独立的观念。在山口大学名誉教授Manabu Takahashi介绍的模型的基础上，建立了一个模型(基因组-分形结构模型)，并用该模型(Cell Prolif)解释了实验结果。为了对细胞中的DNA结构有所启发，我出版了两本书(一本是Manabu Takahashi翻译的书)。这项研究结果的一半将被描述为基因组-分形结构模型。

This study aims to establish polyploid cells and to examine transformation in the cells. We have established polyploid Meth-A cell lines (4-, 6-, 8-, 10-, 12-, 16-and 18-ploid) and polyploid V79 cell lines (3-, 4-and 6-ploid). The transformation through polyploidization has been reported for the temperature dependence, serum concentration dependence and expression of cell-surface hydrocarbon chain.This study intends to change a concept that chromosomes are independent each other in cells. I have made a model (genome-fractal structure model) based on the model that Manabu Takahashi (emeritus professor of Yamaguchi University) has introduced, and explained the experimental results using the model (Cell Prolif. 2002, 2003).In order to enlighten about DNA structure in cells, I have published two books (the one is of translated book of Manabu Takahashi). A half of this RESEARCH RESULTS will be described for the genome-fractal structure model.