Mast Cell-dependent Delayed Hypersensitivity (Jones-Mote type) and Analysis for Cellular Mechanism of the Response.

肥大细胞依赖性迟发性超敏反应（Jones-Mote 型）和细胞反应机制分析。

• 批准号: 63570164
• 负责人: MORIKAWA Shigeru
• 金额: $ 1.34万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570164/
• 关键词: Delayed Hypersensitivity; Jones-Mote type; Mouse; Mast Cell; Macrophage; T cell; Mast Cell-Deficient Mouse; Ferritin; 遅延型アレルギー; ジョンズ; モート型; 足蹠反応

I. *Mast cell-deficient W/W^v mice could develop DH reactions to MHSA and SRBC upon challenge injection on 12th day after subcutaneous sensitization with FCA and antigen. DH reactions of W/W^v and their control +/+ mice were similar however, magnitude of the reactions was found to be lower in W/W^v than +/+ mice. *Mast cells are not essential for elicitation of DH skin responses. *Possibly mast calls regulate the magnitude of the responses.II. *Delayed FPR induced by sensitization with FIA and antigen in PBS were not developed in W/W^v mice 4 weeks after sensitization. On the other hand, +/+ and C57BL/6 mice developed this type of reactions. These FPRs were not suppressed by intraperitoneal injection of ferritin, which was found to suppress elicitation of tuberculin-type of DH response in mice, just before challenge injections. *These observations suggest the presence of two distinct type of DH responses in mice: Mast cell- independent and ferritin sensitive type and mast cell-dependent and ferritin refractory type.

I. * 肥大细胞缺陷的W/W^v小鼠在用FCA和抗原皮下致敏后第12天在攻击注射时可对MHSA和SRBC产生DH反应。W/W^v及其对照+/+小鼠的DH反应相似，然而，发现W/W^v中的反应幅度低于+/+小鼠。* 肥大细胞对于激发DH皮肤反应不是必需的。* 可能桅杆呼叫调节响应的大小。II. * 在致敏后4周，在W/W/W/V小鼠中未出现由PBS中的FIA和抗原致敏诱导的延迟FPR。另一方面，+/+和C57 BL/6小鼠出现了这种类型的反应。这些FPRs没有被抑制的铁蛋白，这被发现抑制激发结核菌素型DH反应的小鼠，就在攻击注射前腹腔注射。* 这些观察结果表明在小鼠中存在两种不同类型的DH应答：肥大细胞非依赖性和铁蛋白敏感型以及肥大细胞依赖性和铁蛋白不应型。

项目成果

Harada,T.: "Abstracts of Workshop Presentations.IVth International Conference on Immunopharmacology." 20 (1988)

Harada,T.：“研讨会演讲摘要。第四届国际免疫药理学会议。”

Torii,I.: "Two distinct types of cellular mechanisms in development of delayed hypersensitivity in mice:Requirement of mast cells and macrophages in elicitaion of the responses." Manuscript.

Torii,I.：“小鼠迟发型超敏反应发展的两种不同类型的细胞机制：引起反应需要肥大细胞和巨噬细胞。”

Torii, I., Morikawa, S., Harada, T., and Nagasaki, M.: "Mast cell-independent inflammatory response of tuberculin-type delayed hypersensitivity in mice."

Torii, I.、Morikawa, S.、Harada, T. 和 Nagasaki, M.：“小鼠结核菌素型迟发型超敏反应的肥大细胞依赖性炎症反应。”

Morikawa,S.: "Cellular Mechanisms in Infection Immunity" Steten Seruminstitute,Copenhagen,Denmark, 25 (1988)

Morikawa,S.：“感染免疫的细胞机制”Steten Seruminstitute，哥本哈根，丹麦，25 (1988)

Torii,I.: "Two distinct types of cellular mechanisms in development of delayed hypersensitivity in mice:Requirement of mast cells and macrophages in elicitation of the responses." Manuscript.

Torii,I.：“小鼠迟发型超敏反应发展的两种不同类型的细胞机制：引发反应需要肥大细胞和巨噬细胞。”