STUDIES ON ORIGIN AND PROPERTIES OF A HODGIN CELL LINE, HPL-Hod-2, WHICH PRODUCE CYTOPLASMIC IgG

霍奇金病是一种独特的恶性淋巴瘤，其特征在于Reed-Sternberg（R-S）细胞和霍奇金（H）细胞的增殖，其正常对应物尚未被鉴定，虽然最近的证据表明是淋巴起源的。本项目旨在阐明R-S和H细胞的正常对应物，并进一步分析其生物学特性，以了解霍奇金病的发病机制。超过21个长期-从恶性淋巴瘤和白血病患者的胸腔积液中建立了HPL-Hod-2（Hod-2）细胞系，Hod-2细胞具有R-S和H细胞共同的细胞标志物，如CD 15，CD 25，Hod-2细胞表面表达CD 83，但其表面免疫球蛋白（IgG）的表达与Hod-2细胞表面表达的IgG分子大小基本相同，但其表面IgG分子大小与Hod-2细胞表面的IgG分子大小基本相同，而Hod-2细胞表面的IgG分子大小与Hod-2细胞表面的IgG分子大小基本相同。CD 83被认为是树突状细胞最可靠的标志物，在21株人恶性淋巴瘤/白血病细胞系中，除Hod-2细胞外，一株毛状白血病细胞系和一株B细胞恶性淋巴瘤细胞系的细胞浆中均弱表达CD 83。

Hodgkin's disease is a unique malignant lymphoma characterized by proliferation of Reed-Sternberg (R-S) cells and Hodgkin (H) cells, whose normal counterpart have not yet been identified, although the most recent evidence suggests a lymphoid origin.This project aim to elucidate normal counterpart of R-S and H cells and further to analysis their biological properties to understand pathogenesis of Hodgkin's disease.More than 21 long-term cultured cell lines derived from patients with malignant lymphoma and leukemia were investigated their immunological properties> Among them, HPL-Hod-2 (Hod-2), a lymphoblastoid cell line had been established from pleural effusion of a patient with Hodgkin's disease> Hod-2 cells shared common cell markers of R-S and H cells such as, CD15, CD25, CD30 and possessed pretty amount of IgG in their cytoplasms.But, they lacked surface immunoglobulin expression.Molecular analysis revealed these IgG consisted of H-chains and k-chains with almost equal molecular size to cytoplasmic IgG of ordinary B-cells.Interestingly enough, it was found that Hod-2 cells express CD83 on their cell surface. CD83 is thought to be the most reliable makers of dendritic cells.Among 21 human malignant lymphoma / leukemia cell lines, one hairly leukemia cell one and one B-cell malignant lymphoma cell line express weakly CD83 in their cytoplasms besides Hod-2 cells.In conclusion ; this investigation revealed the presence of B-lineage DC and their possible original cells of H and R-S cells.