Experimental Studies on the Mechanism of Ethanol Formation in Corpses

为了阐明尸体中乙醇产生的机制，进行了各种体外实验。尽管在室温下仅在血液中产生可忽略不计的乙醇量，但通过向血液中添加葡萄糖，乙醇的量略有增加。当进一步添加腐殖酸时，数量显著增加。将各种材料加入血肝匀浆中作为标本，并将混合物储存在37 ℃的培养箱中。由于每天向混合物中添加抗生素，几乎没有产生任何乙醇。当添加乙醇脱氢酶（ADH）和还原型烟酰胺腺苷二核苷酸（NADH）时，乙醇产量略有增加。当乙醛首先加入时，第二天乙醇的产生受到抑制，但在第2天和之后，乙醇的量比对照材料中的更多。当首先加入异辛酸时，结果与上述相似。吡唑、氨腈和双硫仑完全抑制乙醇的产生。尸体中的乙醇产生被认为是在ADH、ALDH等的影响下通过与活体中乙醇代谢相反的途径发生的，以碳水化合物为底物的腐殖菌。

Various in vitro experiments were performed for the purpose of clarifying the mechanism of ethanol production in corpses. Whereas a negligible quantity of ethanol was produced in the blood alone, which was left at room temperature, the quantity of ethanol was slightly increased by addition of glucose to the blood. When saprogens were further added, the quantity was markedly increased. Various materials were added to blood-liver homogenates as specimens, and the mixtures were stored in an incubator at 37ﾟC. As a result of the addition of an antibiotic to the mixture every day, there was hardly any production of ethanol. When alcohol dehydrogenase (ADH) and reduced nicotinamide adening dinucleotide (NADH) were added, ethanol production was slightly increased. When acetaldehyde was added first, ethanol production was inhibited the next day, but on and after day 2, the quantity of ethanol was more than that in the control material. When pyruvic acid was added first, the results were similar to the above. Pyrazole, cyanamide, and disulfiram completely inhibited the production of ethanol. Ethanol production in corpses is believed to take place through a pathway opposite to that of ethanol metabolism in the living body, under the influence of ADH, ALDH, etc., in saprogens using carbohydrates as substrates.