Experimental Studies on the Mechanism of Ethanol Formation in Corpses

尸体中乙醇形成机制的实验研究

• 批准号: 63570271
• 负责人: NANIKAWA Ryo
• 金额: $ 1.22万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570271/
• 关键词: ethanol; postmortem ethanol; ethanol in corpses; ethanol and saprogens; エタノール; エタノールの死後産生; 死体アルコール; 臓器保存とエタノール産生; n-プロパノール

Various in vitro experiments were performed for the purpose of clarifying the mechanism of ethanol production in corpses. Whereas a negligible quantity of ethanol was produced in the blood alone, which was left at room temperature, the quantity of ethanol was slightly increased by addition of glucose to the blood. When saprogens were further added, the quantity was markedly increased. Various materials were added to blood-liver homogenates as specimens, and the mixtures were stored in an incubator at 37ﾟC. As a result of the addition of an antibiotic to the mixture every day, there was hardly any production of ethanol. When alcohol dehydrogenase (ADH) and reduced nicotinamide adening dinucleotide (NADH) were added, ethanol production was slightly increased. When acetaldehyde was added first, ethanol production was inhibited the next day, but on and after day 2, the quantity of ethanol was more than that in the control material. When pyruvic acid was added first, the results were similar to the above. Pyrazole, cyanamide, and disulfiram completely inhibited the production of ethanol. Ethanol production in corpses is believed to take place through a pathway opposite to that of ethanol metabolism in the living body, under the influence of ADH, ALDH, etc., in saprogens using carbohydrates as substrates.

为了阐明乙醇在尸体中产生的机制，我们进行了各种体外实验。然而，在室温下，血液中产生的乙醇量可以忽略不计，乙醇的量通过向血液中添加葡萄糖而略有增加。随着腐殖酸的进一步添加，腐殖酸的数量显著增加。将各种材料加入血肝匀浆中作为标本，放入37℃的培养箱中保存。由于每天在混合物中添加抗生素，几乎没有任何乙醇的生产。添加醇脱氢酶（ADH）和还原性烟酰胺加甲二核苷酸（NADH）后，乙醇产量略有增加。第一次加入乙醛时，第二天乙醇产量受到抑制，但在第2天及之后，乙醇的数量多于对照材料。当先加入丙酮酸时，结果与上述相似。吡唑、氰酰胺和二硫仑完全抑制乙醇的产生。尸体中乙醇的产生被认为是通过与生物体内乙醇代谢相反的途径进行的，在以碳水化合物为底物的腐菌中受ADH、ALDH等的影响。

项目成果

Ryo Nanikawa,Fumio Moriya & Y.Hashimoto: "Experimental Studies on the Mechanism of Ethanol Formation in Corpses" Zeitschrift fur Rechtsmedizin. 101. 21-26 (1988)

浪川亮、守谷文雄

Ryo Nanikawa, Fumio Moriya, and Yoshiaki Hashimoto: "Experimental Studies on the Mechanism of Ethanol Formation in Corpses" Zeitschrift fur Rechtsmedizin. 101. 21-26 (1988)

Ryo Nanikawa、Fumio Moriya 和 Yoshiaki Hashimoto：“尸体中乙醇形成机制的实验研究”Zeitschrift Fur Rechtsmedizin。