Study on host factors in lung cancer patients

这项研究的目的是检查肺癌患者和癌症高危个体患肿瘤的风险。苯并(A)芘(BP)对淋巴细胞姐妹染色单体互换(SCES)的诱导性：肺癌患者淋巴细胞培养的姐妹染色单体互换(SCES)频率明显高于健康人或低危人群。暴露于BP后，肺癌患者和高危人群的淋巴细胞姐妹染色单体交换效率显著高于低危人群。这一发现表明，高危个体的淋巴细胞可能比低危个体的淋巴细胞更容易受到BP诱导的DNA损伤。Ras癌基因产物的表达：采用MobAbrp35检测切除肺肿瘤组织切片中ras p21的表达。原发灶直径大于30 mm的肿瘤材料对RP-35呈强阳性反应的频率显著高于原发灶直径小于或等于30 mm的肿瘤组织。肿瘤染色阴性或不明确的患者的生存时间明显长于中度染色或浓染肿瘤的患者。这些结果提示ras癌基因可能在肺癌原发部位的生长或肿瘤发生中起重要作用。家庭因素对微量营养素的影响：在家庭成员中，特别是腺癌患者的家庭成员中，血清硒和维生素E水平显著低于对照组。提示肺癌患者家族血清硒、维生素E水平存在家族因素。

The purpose of this study is to examine the risk of neoplasma in lung cancer patients and high-cancer-risk individuals.1. The inducibility of sister chromatid exchange(SCEs) by benzo(a)pyrene(BP): The baseline SCE frequency in cultured lymphocytes was significantly high in lung cancer patients, as compared with all healthy persons or low-cancer-risk individuals. Following exposure to BP, the lymphocytes of lung-cancer patients and high-cancer-risk individuals exhibited significantly greater SCE yields than those of persons at low risk. This findings suggest that lymphocytes of high-risk individuals may be more susceptible to BP-induced DNA damage than those of persons at low risk.2. Expression of ras oncogene product: MobAb rp35 was used for detection of ras p21 in histologic section of resected lung tumors. Materials of tumors from primary sites larger than 30 mm in diameter exhibited intensely positive reactions with rp-35 significantly more frequently than those with primary sites, 30 mm in diameter or smaller. Patients with negatively or equivocally staining tumor had significantly longer survival times than did those with moderately staining tumor or those with intensely staining tumor. These results suggest that ras oncogene may play a significant role in growth or tumorigenesis at the primary site in lung cancer.3. Familial factors in micronutrients: Among Family members, particularly, families of adenocarcinoma patients, serum selenium and vitamin E levels were significantly lower than those in controls. These data suggest that there are familial factors in serum selenium and vitamin E levels among families of lung cancer patients.